Using proximity loggers in studies of sheep behavior: An estimation of the daily frequency of water consumption

Abstract

Background Proximity loggers are a biotelemetry technology used to quantify animal-animal interactions non-invasively. They are small devices can be affixed to a target animal and detects and records if another device is within a certain distance, which can provide unprecedented insights into individual behavior and interactions with other animals or other aspects of the environment. Proximity loggers also can record the duration and distance between the two or more individuals detected, and have been used to study a diverse array of interactions. Studying drinking behavior in sheep is important for several critical reasons, such as improving animal welfare, enhancing productivity, managing resources efficiently, and ensuring sustainable agricultural practices. The objective of this study was to test the capacity for Bluetooth Low Energy proximity loggers to quantify drinking habits in sheep (n = 7 adult females). At all times, they had access to water from two drinking troughs. Animals wore neck collars that contained a mobile logger, and two stationary loggers were placed under the drinking troughs. To store the records downloaded from the stationary loggers, a gateway was placed close to the troughs. The mobile loggers were programmed as beacons to transmit their ID to the stationary loggers, which were programmed as receivers to scan the proximity of the mobile loggers, record the time, the IDs of the mobile loggers, and the received signal strength of the contact with the mobile loggers. Results Ewes visited the drinking troughs 2.4 +/- 0.5 times per day (rank 1.4-2.9), most frequently (42%) between 0800 and 1000 h, and 84% occurred in the daytime. Mean length of each water intake was 1.72 +/- 0.30 min. The cosinor analysis of the distribution of drinking events revealed a significant (P < 0.0001) 24-h circadian rhythm in drinking events, and the acrophase (time of the highest proportion of events) occurred at 1054 h. Conclusions Considering that studying water intake in sheep is crucial for optimizing their health, welfare, and management, the proximity loggers were demonstrated to be appropriate for documenting the drinking habits of sheep, and probably, would be suitable for studies of other behaviors that involve approaching other animals or objects.