Publication:
Biofilm forming capacity and antibiotic susceptibility of Staphylococcus spp. with the icaA/icaD/bap genotype isolated from ocular surface of patients with diabetes

dc.contributor.authorArık, Gizem
dc.contributor.authorKıvanç, Merih
dc.contributor.buuauthorKıvanç, Sertaç Argün
dc.contributor.buuauthorAkova, Berna Budak
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentGöz Hastalıkları Ana Bilim Dalı
dc.contributor.orcid0000-0002-0932-6977
dc.contributor.orcid0000-0003-0995-5260
dc.contributor.researcheridAAH-6518-2021
dc.contributor.researcheridABE-3033-2020
dc.contributor.scopusid47861204900
dc.contributor.scopusid55370489800
dc.date.accessioned2023-10-27T06:51:55Z
dc.date.available2023-10-27T06:51:55Z
dc.date.issued2018-12
dc.description.abstractIntroduction Bacterial biofilm is an exopolysaccharide matrix that is produced by bacteria while they adhere on abiotic or biotic surfaces. The bacteria living in this matrix are more resistant to antibiotics than planctonic bacteria. The biofilm formation property of the bacteria is determined by genes; and this is related to virulence of the microorganism. In ophthalmology, biofilms form especially on abiotic surfaces such as silicon tubes, contact lenses, intraocular lenses etc. Aim Our aim was to investigate genotypic and phenotypic structures of biofilms that are produced by Staphylococcus spp., which was obtained from the eyes of diabetic patients and determine the effect on antibiotic susceptibility. Methods The study group was comprised with 83 isolates from diabetic patients and 21 isolates from non-diabetic patients. Presumptive isolates were detected and confirmed by a microbial identification system VITEK II. Automated EcoRI Ribotyping was performed. Biofilm production was detected by Congo Red Agar Plate and Microtiter Plate Assay. Disc diffusion method was used for determination of antibiotic susceptibility of isolates. Results Out of the 83 isolates from diabetic patients, 25 were weakly (30%), 20 were moderately (24%), and 25 were strongly (30%) biofilm positive. Seven isolates of S. aureus, 11 isolates of S. epidermidis, 2 isolates of S. warneri, 3 isolates of S. hominis, and 2 isolates of S. lugdunensis were identified as strong biofilm producers. Out of the 83 Staphylococcus isolates, 37 were cefuroxime, 18 ciprofloxacin, 11 vancomycin, 12 gatifloxacin, and 18 moxifloxacin resistant. In total, 37 strains were resistant to three or more antibiotics. There was a statistically significant relation between biofilm formation and multidrug resistance (against three or more antibiotics,p<0.001). In nondiabetic patients, 15(71%) isolates were non adherent or weakly adherent, and 2(10%) were strongly adherent biofilm positive. Conclusion In conclusion, bacterial conjunctival flora of patients with diabetes is likely to produce biofilm. Biofilm formation is associated with multidrug rsistance in patients with diabetes.
dc.description.sponsorshipAnadolu Üniversitesi Bilimsel Araştırma Proje Fonu - 1306F164
dc.identifier.citationKıvanç, S. A. vd. (2018). ''Biofilm forming capacity and antibiotic susceptibility of Staphylococcus spp. with the icaA/icaD/bap genotype isolated from ocular surface of patients with diabetes''. Malawi Medical Journal, 30(4), 243-249.
dc.identifier.endpage249
dc.identifier.issn1995-7262
dc.identifier.issn1995-7270
dc.identifier.issue4
dc.identifier.pubmed31798802
dc.identifier.scopus2-s2.0-85060846745
dc.identifier.startpage243
dc.identifier.urihttps://doi.org/10.4314/mmj.v30i4.6
dc.identifier.urihttps://www.ajol.info/index.php/mmj/article/view/181967
dc.identifier.urihttp://hdl.handle.net/11452/34607
dc.identifier.volume30
dc.identifier.wos000456650500006
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherKamuzu University Health Sciences
dc.relation.collaborationYurt içi
dc.relation.journalMalawi Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPublic, environmental & occupational health
dc.subjectDiabetes
dc.subjectBiofilm
dc.subjectStaphylococcus spp
dc.subjectAntibiotic resistance
dc.subjectOcular surface
dc.subjectCoagulase-negative staphylococci
dc.subjectCataract-surgery
dc.subjectSlime production
dc.subjectEpidermidis
dc.subjectEndophthalmitis
dc.subjectPathogenesis
dc.subjectResistance
dc.subjectStrains
dc.subjectAureus
dc.subjectFlora
dc.subject.emtreeAntiinfective agent
dc.subject.emtreeBacterial protein
dc.subject.emtreeAntibiotic resistance
dc.subject.emtreeBacterial eye infection
dc.subject.emtreeBacterial gene
dc.subject.emtreeBiofilm
dc.subject.emtreeDiabetes mellitus
dc.subject.emtreeDiabetic complication
dc.subject.emtreeDrug effect
dc.subject.emtreeGenetics
dc.subject.emtreeGenotype
dc.subject.emtreeGrowth, development and aging
dc.subject.emtreeHuman
dc.subject.emtreeIsolation and purification
dc.subject.emtreeMicrobial sensitivity test
dc.subject.emtreeMicrobiology
dc.subject.emtreePhenotype
dc.subject.emtreePhysiology
dc.subject.emtreePolymerase chain reaction
dc.subject.emtreeRetrospective study
dc.subject.emtreeScanning electron microscopy
dc.subject.emtreeStaphylococcus
dc.subject.emtreeStaphylococcus infection
dc.subject.meshAnti bacterial agents
dc.subject.meshBacterial proteins
dc.subject.meshBiofilms
dc.subject.meshDiabetes complications
dc.subject.meshDiabetes mellitus
dc.subject.meshDrug resistance, bacterial
dc.subject.meshEye infections, bacterial
dc.subject.meshGenes, bacterial
dc.subject.meshGenotype
dc.subject.meshHumans
dc.subject.meshMicrobial sensitivity tests
dc.subject.meshMicroscopy, electron, scanning
dc.subject.meshPhenotype
dc.subject.meshPolymerase chain reaction
dc.subject.meshRetrospective studies
dc.subject.meshStaphylococcal infections
dc.subject.meshStaphylococcus
dc.subject.scopusStaphylococcus Epidermidis; Anti-Bacterial Agents; Polysaccharide Intercellular Adhesin
dc.subject.wosPublic, environmental & occupational health
dc.titleBiofilm forming capacity and antibiotic susceptibility of Staphylococcus spp. with the icaA/icaD/bap genotype isolated from ocular surface of patients with diabetes
dc.typeArticle
dc.wos.quartileQ4
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Göz Hastalıkları Ana Bilim Dalı
local.indexed.atScopus
local.indexed.atWOS

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