Publication:
In vitro DNA binding studies of the sweetening agent saccharin and its copper(II) and zinc(II) complexes

Placeholder

Organizational Units

Authors

İçsel, Ceyda
Yılmaz, Veysel Turan

Authors

Advisor

Language

Publisher:

Elsevier

Journal Title

Journal ISSN

Volume Title

Abstract

The interactions of fish sperm DNA (FS-DNA) with the sodium salt of sweetener saccharin (sacH) and its copper and zinc complexes, namely [M(sac)(2)(H2O)(4)]center dot 2H(2)O (M = Cu-II or Zn-II) were studied by using UV-Vis titration, fluorometric competition, thermal denaturation, viscosity and gel electrophoresis measurements. The intrinsic binding constants (K-b) obtained from absorption titrations were estimated to be 2.86 (+/-0.06) x 10(4) M-1 for Na(sac), 6.67 (+/-0.12) x 10(4) M-1 for Cu-sac and 4.01 (+/-0.08) x 10(4)M(-1) for Zn-sac. The Cu-sac complex binds to FS-DNA via intercalation with a K-A value of 50.12 (+/-0.22) x 10(4) M-1 as evidenced by competitive binding studies with ethidium bromide. Moreover, competition experiments with Hoechst 33258 are indicative of a groove binding mode of Na(sac) and Zn-sac with binding constants of 3.13 (+/-0.16) x 10(4) M-1 and 5.25 (0.22) x 10(4) M-1, respectively. The spectroscopic measurements indicate a moderate DNA binding affinity of Na(sac) and its metal complexes. The suggested binding modes are further confirmed by the thermal denaturation and viscosity measurements. In addition, Cu-sac and Zn-sac show weak ability to damage to pBR322 supercoiled plasmid DNA.

Description

Source:

Keywords:

Keywords

Saccharin, DNA binding, Copper(II) saccharinate, Zinc(II) saccharinate, UV-Vis spectroscopy, Fluorescence spectroscopy, Acridine-orange, Platinum(II) complexes, Ethidium-bromide, Inutero-exposure, Metal-complexes, Palladium(II), Cancer, Crystal, Intercalation, Recognition, Biochemistry & molecular biology, Biophysics

Citation

İçsel, C. ve Yılmaz, V. T. (2014). "In vitro DNA binding studies of the sweetening agent saccharin and its copper(II) and zinc(II) complexes". Journal of Photochemistry and Photobiology B: Biology, 130, 115-121.

Endorsement

Review

Supplemented By

Referenced By

0

Views

0

Downloads