Resistance status in Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae) populations against single and mixture of neonicotinoid and synthetic pyrethroid insecticides

Abstract

The peach potato aphid, Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae), a vector of many plant virus diseases, causes damage to its wide range of hosts by direct feeding. Chemical control has been the primary method to control this species, and the intensive use of insecticides has led to the development of resistance. In this study, conducted between the years 2017-2019, firstly resistance ratio of five M. persicae populations from Antalya, Turkiye were determined by leaf-dip bioassay method. The field populations showed significant resistance to thiamethoxam (between 201-332 fold) and lambda-cyhalothrin (between 50-103 fold) when compared to susceptible population. To identify whether resistance mediated by mutations in sodium channel and nicotinic acetylcholine receptor, DNA regions that encompass "mutation hot-spot" were sequenced. This revealed no population contained R81T mutation that has been previously linked with neonicotinoid resistance. As to synthetic pyrethroid resistance, the L1014F kdr mutation was fixed in all field populations. This study is the first description of kdr mutation in M. persicae populations from Turkiye. Bioassay results also indicated that the toxicity of thiamethoxam and lambda-cyhalothrin mixture was higher than that of lambda-cyhalothrin alone. Our findings can make significant contributions to M. persicae resistance management.