Publication:
Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender

dc.contributor.buuauthorÜstüner, Burcu
dc.contributor.buuauthorAlcay, Selim
dc.contributor.buuauthorToker, M. Berk
dc.contributor.buuauthorNur, Zekariya
dc.contributor.buuauthorGökce, Elif
dc.contributor.buuauthorSonat, Füsun Ak
dc.contributor.buuauthorGül, Zülfiye
dc.contributor.buuauthorDuman, Muhammed
dc.contributor.buuauthorCenize, Cafer
dc.contributor.buuauthorUslu, Aydın
dc.contributor.buuauthorSaığrkaya, Hakan
dc.contributor.buuauthorSoylu, Mustafa Kemal
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.departmentFizyoloji Ana Bilim Dalı
dc.contributor.departmentFarmakoloji Ana Bilim Dalı
dc.contributor.departmentÜreme ve Suni Tohumlama Ana Bilim Dalı
dc.contributor.departmentSu Hayvanları Hastalıkları Ana Bilim Dalı
dc.contributor.orcid0000-0001-7707-2705
dc.contributor.orcid0000-0002-1438-221X
dc.contributor.orcid0000-0002-8872-0074
dc.contributor.orcid0000-0002-7678-3289
dc.contributor.researcheridAAH-2635-2021
dc.contributor.researcheridT-1697-2019
dc.contributor.researcheridAAH-8821-2021
dc.contributor.researcheridAAF-9939-2020
dc.contributor.researcheridAAG-7238-2021
dc.contributor.scopusid18937724600
dc.contributor.scopusid56099810300
dc.contributor.scopusid56480349200
dc.contributor.scopusid6508060684
dc.contributor.scopusid56779799700
dc.contributor.scopusid26428428000
dc.contributor.scopusid56086542900
dc.contributor.scopusid55568071100
dc.contributor.scopusid57070339500
dc.contributor.scopusid57069822700
dc.contributor.scopusid6602400461
dc.contributor.scopusid7003293300
dc.date.accessioned2022-10-27T07:27:09Z
dc.date.available2022-10-27T07:27:09Z
dc.date.issued2015-11-15
dc.description.abstractThe aim of the current study was to evaluate the effects of different concentrations of rainbow trout seminal plasma (RTSP) (0.1%, 1% and 10%) in extenders containing either egg yolk or lecithin for use in Awassi ram semen cryopreservation. Pooled sperm were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in egg yolk or lecithin extender containing no RTSP, 0.1%, 1% or 10% RTSP (v/v). Semen samples were assessed for sperm motility, plasma membrane integrity [hypoosmotic swelling test (HOST) and Hoechst 33258] and defective acrosomes [FITC-conjugated Pisum sativum agglutinin (PSA-FITC)] at the following five time points: after dilution with extender A; after equilibration; and post-thaw at 0 h, 3 h and 5 h. Malondialdehyde (MDA) was examined only after thawing. Freezing and thawing procedures (dilution, equilibration and post-thaw incubation at Oh, 3 h and 5 h) negatively affected the motility (P<0.001) and acrosome integrity (P<0.001). Additionally, freezing and thawing negatively affected the plasma membrane integrity, as determined by the HOST and Hoechst 33258 (P<0.001). The extender group affected the motility (P<0.001) and the HOST results (P<0.001). Levels of MDA in the egg yolk extender with 1% RTSP group were significantly lower than in the lecithin control group (P<0.05). In conclusion, the egg yolk extender groups that were supplemented with 10% and 1% RTSP provided greater cryoprotective effects for semen survivability during 5 h incubation than the other extender groups.
dc.identifier.citationÜstüner, B. vd. (2016). "Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender". Animal Reproduction Science, 164, 97-104.
dc.identifier.endpage104
dc.identifier.issn0378-4320
dc.identifier.issn1873-2232
dc.identifier.pubmed26685096
dc.identifier.scopus2-s2.0-84955076104
dc.identifier.startpage97
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2015.11.017
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0378432015300610
dc.identifier.urihttp://hdl.handle.net/11452/29227
dc.identifier.volume164
dc.identifier.wos000369562900013
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherElsevier
dc.relation.bapKUAP-V 2013/10
dc.relation.journalAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAgriculture
dc.subjectReproductive biology
dc.subjectVeterinary sciences
dc.subjectRam semen
dc.subjectCryopreservation
dc.subjectRainbow trout
dc.subjectSeminal plasma
dc.subjectHypoosmotic swelling test
dc.subjectIn-vitro
dc.subjectCold-shock
dc.subjectSperm
dc.subjectSpermatozoa
dc.subjectParameters
dc.subjectFertility
dc.subjectCapacitation
dc.subjectUnfrozen
dc.subjectGlycerol
dc.subject.emtreeCryoprotective agent
dc.subject.emtreeMalonaldehyde
dc.subject.emtreePlant lectin
dc.subject.emtreeSoybean lectin
dc.subject.emtreeSoybean protein
dc.subject.emtreeAcrosome
dc.subject.emtreeAnimal
dc.subject.emtreeCell membrane
dc.subject.emtreeCryopreservation
dc.subject.emtreeEgg yolk
dc.subject.emtreeMale
dc.subject.emtreeOncorhynchus mykiss
dc.subject.emtreePhysiology
dc.subject.emtreeSheep
dc.subject.emtreeSperm
dc.subject.emtreeSperm preservation
dc.subject.emtreeVeterinary
dc.subject.meshAcrosome
dc.subject.meshAnimals
dc.subject.meshCell membrane
dc.subject.meshCryopreservation
dc.subject.meshCryoprotective agents
dc.subject.meshEgg yolk
dc.subject.meshMale
dc.subject.meshMalondialdehyde
dc.subject.meshOncorhynchus mykiss
dc.subject.meshPlant lectins
dc.subject.meshSemen
dc.subject.meshSemen preservation
dc.subject.meshSheep
dc.subject.meshSoybean proteins
dc.subject.scopusExtender; Semen; Semen Extenders
dc.subject.wosAgriculture, dairy & animal science
dc.subject.wosReproductive biology
dc.subject.wosVeterinary sciences
dc.titleEffect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender
dc.typeArticle
dc.wos.quartileQ1 (Agriculture, dairy & animal science)
dc.wos.quartileQ4 (Reproductive biology)
dspace.entity.typePublication
local.contributor.departmentVeteriner Fakültesi/Üreme ve Suni Tohumlama Ana Bilim Dalı
local.contributor.departmentVeteriner Fakültesi/Fizyoloji Ana Bilim Dalı
local.contributor.departmentTıp Fakültesi/Farmakoloji Ana Bilim Dalı
local.contributor.departmentVeteriner Fakültesi/Su Hayvanları Hastalıkları Ana Bilim Dalı
local.indexed.atPubMed
local.indexed.atWOS

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