Publication: Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender
dc.contributor.buuauthor | Üstüner, Burcu | |
dc.contributor.buuauthor | Alcay, Selim | |
dc.contributor.buuauthor | Toker, M. Berk | |
dc.contributor.buuauthor | Nur, Zekariya | |
dc.contributor.buuauthor | Gökce, Elif | |
dc.contributor.buuauthor | Sonat, Füsun Ak | |
dc.contributor.buuauthor | Gül, Zülfiye | |
dc.contributor.buuauthor | Duman, Muhammed | |
dc.contributor.buuauthor | Cenize, Cafer | |
dc.contributor.buuauthor | Uslu, Aydın | |
dc.contributor.buuauthor | Saığrkaya, Hakan | |
dc.contributor.buuauthor | Soylu, Mustafa Kemal | |
dc.contributor.department | Tıp Fakültesi | |
dc.contributor.department | Veteriner Fakültesi | |
dc.contributor.department | Veteriner Fakültesi | |
dc.contributor.department | Veteriner Fakültesi | |
dc.contributor.department | Fizyoloji Ana Bilim Dalı | |
dc.contributor.department | Farmakoloji Ana Bilim Dalı | |
dc.contributor.department | Üreme ve Suni Tohumlama Ana Bilim Dalı | |
dc.contributor.department | Su Hayvanları Hastalıkları Ana Bilim Dalı | |
dc.contributor.orcid | 0000-0001-7707-2705 | |
dc.contributor.orcid | 0000-0002-1438-221X | |
dc.contributor.orcid | 0000-0002-8872-0074 | |
dc.contributor.orcid | 0000-0002-7678-3289 | |
dc.contributor.researcherid | AAH-2635-2021 | |
dc.contributor.researcherid | T-1697-2019 | |
dc.contributor.researcherid | AAH-8821-2021 | |
dc.contributor.researcherid | AAF-9939-2020 | |
dc.contributor.researcherid | AAG-7238-2021 | |
dc.contributor.scopusid | 18937724600 | |
dc.contributor.scopusid | 56099810300 | |
dc.contributor.scopusid | 56480349200 | |
dc.contributor.scopusid | 6508060684 | |
dc.contributor.scopusid | 56779799700 | |
dc.contributor.scopusid | 26428428000 | |
dc.contributor.scopusid | 56086542900 | |
dc.contributor.scopusid | 55568071100 | |
dc.contributor.scopusid | 57070339500 | |
dc.contributor.scopusid | 57069822700 | |
dc.contributor.scopusid | 6602400461 | |
dc.contributor.scopusid | 7003293300 | |
dc.date.accessioned | 2022-10-27T07:27:09Z | |
dc.date.available | 2022-10-27T07:27:09Z | |
dc.date.issued | 2015-11-15 | |
dc.description.abstract | The aim of the current study was to evaluate the effects of different concentrations of rainbow trout seminal plasma (RTSP) (0.1%, 1% and 10%) in extenders containing either egg yolk or lecithin for use in Awassi ram semen cryopreservation. Pooled sperm were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in egg yolk or lecithin extender containing no RTSP, 0.1%, 1% or 10% RTSP (v/v). Semen samples were assessed for sperm motility, plasma membrane integrity [hypoosmotic swelling test (HOST) and Hoechst 33258] and defective acrosomes [FITC-conjugated Pisum sativum agglutinin (PSA-FITC)] at the following five time points: after dilution with extender A; after equilibration; and post-thaw at 0 h, 3 h and 5 h. Malondialdehyde (MDA) was examined only after thawing. Freezing and thawing procedures (dilution, equilibration and post-thaw incubation at Oh, 3 h and 5 h) negatively affected the motility (P<0.001) and acrosome integrity (P<0.001). Additionally, freezing and thawing negatively affected the plasma membrane integrity, as determined by the HOST and Hoechst 33258 (P<0.001). The extender group affected the motility (P<0.001) and the HOST results (P<0.001). Levels of MDA in the egg yolk extender with 1% RTSP group were significantly lower than in the lecithin control group (P<0.05). In conclusion, the egg yolk extender groups that were supplemented with 10% and 1% RTSP provided greater cryoprotective effects for semen survivability during 5 h incubation than the other extender groups. | |
dc.identifier.citation | Üstüner, B. vd. (2016). "Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender". Animal Reproduction Science, 164, 97-104. | |
dc.identifier.endpage | 104 | |
dc.identifier.issn | 0378-4320 | |
dc.identifier.issn | 1873-2232 | |
dc.identifier.pubmed | 26685096 | |
dc.identifier.scopus | 2-s2.0-84955076104 | |
dc.identifier.startpage | 97 | |
dc.identifier.uri | https://doi.org/10.1016/j.anireprosci.2015.11.017 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0378432015300610 | |
dc.identifier.uri | http://hdl.handle.net/11452/29227 | |
dc.identifier.volume | 164 | |
dc.identifier.wos | 000369562900013 | |
dc.indexed.wos | SCIE | |
dc.language.iso | en | |
dc.publisher | Elsevier | |
dc.relation.bap | KUAP-V 2013/10 | |
dc.relation.journal | Animal Reproduction Science | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Agriculture | |
dc.subject | Reproductive biology | |
dc.subject | Veterinary sciences | |
dc.subject | Ram semen | |
dc.subject | Cryopreservation | |
dc.subject | Rainbow trout | |
dc.subject | Seminal plasma | |
dc.subject | Hypoosmotic swelling test | |
dc.subject | In-vitro | |
dc.subject | Cold-shock | |
dc.subject | Sperm | |
dc.subject | Spermatozoa | |
dc.subject | Parameters | |
dc.subject | Fertility | |
dc.subject | Capacitation | |
dc.subject | Unfrozen | |
dc.subject | Glycerol | |
dc.subject.emtree | Cryoprotective agent | |
dc.subject.emtree | Malonaldehyde | |
dc.subject.emtree | Plant lectin | |
dc.subject.emtree | Soybean lectin | |
dc.subject.emtree | Soybean protein | |
dc.subject.emtree | Acrosome | |
dc.subject.emtree | Animal | |
dc.subject.emtree | Cell membrane | |
dc.subject.emtree | Cryopreservation | |
dc.subject.emtree | Egg yolk | |
dc.subject.emtree | Male | |
dc.subject.emtree | Oncorhynchus mykiss | |
dc.subject.emtree | Physiology | |
dc.subject.emtree | Sheep | |
dc.subject.emtree | Sperm | |
dc.subject.emtree | Sperm preservation | |
dc.subject.emtree | Veterinary | |
dc.subject.mesh | Acrosome | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Cell membrane | |
dc.subject.mesh | Cryopreservation | |
dc.subject.mesh | Cryoprotective agents | |
dc.subject.mesh | Egg yolk | |
dc.subject.mesh | Male | |
dc.subject.mesh | Malondialdehyde | |
dc.subject.mesh | Oncorhynchus mykiss | |
dc.subject.mesh | Plant lectins | |
dc.subject.mesh | Semen | |
dc.subject.mesh | Semen preservation | |
dc.subject.mesh | Sheep | |
dc.subject.mesh | Soybean proteins | |
dc.subject.scopus | Extender; Semen; Semen Extenders | |
dc.subject.wos | Agriculture, dairy & animal science | |
dc.subject.wos | Reproductive biology | |
dc.subject.wos | Veterinary sciences | |
dc.title | Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender | |
dc.type | Article | |
dc.wos.quartile | Q1 (Agriculture, dairy & animal science) | |
dc.wos.quartile | Q4 (Reproductive biology) | |
dspace.entity.type | Publication | |
local.contributor.department | Veteriner Fakültesi/Üreme ve Suni Tohumlama Ana Bilim Dalı | |
local.contributor.department | Veteriner Fakültesi/Fizyoloji Ana Bilim Dalı | |
local.contributor.department | Tıp Fakültesi/Farmakoloji Ana Bilim Dalı | |
local.contributor.department | Veteriner Fakültesi/Su Hayvanları Hastalıkları Ana Bilim Dalı | |
local.indexed.at | PubMed | |
local.indexed.at | WOS |
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