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Evaluation of in vitro cytotoxicity and genotoxicity of copper-zinc alloy nanoparticles in human lung epithelial cells

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dc.contributor.authorKumbıçak, Ümit
dc.contributor.authorKumbıçak, Zübeyde Akan
dc.contributor.buuauthorÇavaş, Tolga
dc.contributor.buuauthorÇinkılıç, Nilüfer
dc.contributor.buuauthorVatan, Özgür
dc.contributor.buuauthorYılmaz, Dilek
dc.contributor.departmentFen Edebiyat Fakültesi
dc.contributor.departmentBiyoloji Bölümü
dc.contributor.orcid0000-0003-1620-1918
dc.contributor.orcid0000-0002-7687-3284
dc.contributor.orcid0000-0002-3595-6286
dc.contributor.researcheridAAH-3508-2021
dc.contributor.researcheridO-7508-2015
dc.contributor.researcheridAAH-5296-2021
dc.contributor.scopusid6602989548
dc.contributor.scopusid26533892300
dc.contributor.scopusid16235098100
dc.contributor.scopusid6701369462
dc.date.accessioned2022-09-06T07:26:02Z
dc.date.available2022-09-06T07:26:02Z
dc.date.issued2014-11
dc.description.abstractIn the present study, in vitro cytotoxic and genotoxic effect of copper-zinc alloy nanoparticles (Cu-Zn ANPs) on human lung epithelial cells (BEAS-2B) were investigated. XTT test and clonogenic assay were used to determine cytotoxic effects. Cell death mode and intracellular reactive oxygen species formations were analyzed using M30, M65 and ROS Elisa assays. Genotoxic effects were evaluated using micronucleus, comet and gamma-H2AX foci assays. Cu-Zn ANPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential measurements. Characterization of Cu-Zn ANPs showed an average size of 200 nm and zeta potential of -22 mV. TEM analyses further revealed the intracellular localization of Cu-Zn ANPs in cytoplasm within 24 h. Analysis of micronucleus, comet and gamma-H2AX foci counts showed that exposure to Cu-Zn ANPs significantly induced chromosomal damage as well as single and double stranded DNA damage in BEAS-2B cells. Our results further indicated that exposure to Cu-Zn ANPs significantly induced intracellular ROS formation. Evaluation of M30:M65 ratios suggested that cell death was predominantly due to necrosis.
dc.identifier.citationKumbıçak, Ü. vd. (2014). "Evaluation of in vitro cytotoxicity and genotoxicity of copper-zinc alloy nanoparticles in human lung epithelial cells". Food and Chemical Toxicology, 73, 105-112.
dc.identifier.doi10.1016/j.fct.2014.07.040
dc.identifier.endpage112
dc.identifier.issn0278-6915
dc.identifier.issn1873-6351
dc.identifier.pubmed25116682
dc.identifier.scopus2-s2.0-84908068377
dc.identifier.startpage105
dc.identifier.urihttps://doi.org/10.1016/j.fct.2014.07.040
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S027869151400372X
dc.identifier.urihttp://hdl.handle.net/11452/28490
dc.identifier.volume73
dc.identifier.wos000345491500012
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherPergamon-Elsevier Science
dc.relation.bapKUAP F-2012/61
dc.relation.collaborationYurt içi
dc.relation.journalFood and Chemical Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectNanotoxicology
dc.subjectCopper-zinc alloy nanoparticles
dc.subjectBEAS-2B cells
dc.subjectCytotoxicity
dc.subjectGenotoxicity
dc.subjectMetal-oxide nanoparticles
dc.subjectZno nanoparticles
dc.subjectDNA-damage
dc.subjectToxicity
dc.subjectNanomaterials
dc.subjectRelease
dc.subjectNickel
dc.subjectCUO
dc.subjectFood science & technology
dc.subjectToxicology
dc.subject.emtreeCopper nanoparticle
dc.subject.emtreeHistone H2AX
dc.subject.emtreeReactive oxygen metabolite
dc.subject.emtreeZinc oxide nanoparticle
dc.subject.emtreeCopper
dc.subject.emtreeMetal nanoparticle
dc.subject.emtreeReactive oxygen metabolite
dc.subject.emtreeZinc
dc.subject.emtreeApoptosis
dc.subject.emtreeArticle
dc.subject.emtreeCell viability
dc.subject.emtreeCellular distribution
dc.subject.emtreeChromosome damage
dc.subject.emtreeControlled study
dc.subject.emtreeCytotoxicity
dc.subject.emtreeDNA damage
dc.subject.emtreeDouble stranded DNA break
dc.subject.emtreeGenotoxicity
dc.subject.emtreeHuman
dc.subject.emtreeHuman cell
dc.subject.emtreeIC50
dc.subject.emtreeIn vitro study
dc.subject.emtreeLung alveolus epithelium
dc.subject.emtreeMicronucleus
dc.subject.emtreeNecrosis
dc.subject.emtreeOxidative stress
dc.subject.emtreeParticle size
dc.subject.emtreeZeta potential
dc.subject.emtreeCarcinogen testing
dc.subject.emtreeChemistry
dc.subject.emtreeCytology
dc.subject.emtreeDrug effects
dc.subject.emtreeEnzyme linked immunosorbent assay
dc.subject.emtreeEpithelium cell
dc.subject.emtreeLung
dc.subject.emtreeMetabolism
dc.subject.emtreeMutagen testing
dc.subject.emtreeTransmission electron microscopy
dc.subject.meshApoptosis
dc.subject.meshCarcinogenicity tests
dc.subject.meshCopper
dc.subject.meshEnzyme-linked immunosorbent assay
dc.subject.meshEpithelial cells
dc.subject.meshHumans
dc.subject.meshIn vitro techniques
dc.subject.meshLung
dc.subject.meshMetal nanoparticles
dc.subject.meshMicroscopy, electron, transmission
dc.subject.meshMutagenicity tests
dc.subject.meshReactive oxygen species
dc.subject.meshZinc
dc.subject.scopusZinc Oxide Nanoparticle; Antibacterial Activity; Zinc Nitrate
dc.subject.wosFood science & technology
dc.subject.wosToxicology
dc.titleEvaluation of in vitro cytotoxicity and genotoxicity of copper-zinc alloy nanoparticles in human lung epithelial cells
dc.typeArticle
dc.wos.quartileQ1 (Food science & technology)
dc.wos.quartileQ2 (Toxicology)
dspace.entity.typePublication
local.contributor.departmentFen Edebiyat Fakültesi/Biyoloji Bölümü
local.indexed.atScopus
local.indexed.atWOS

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