Publication:
Screening for high callus induction and agrobacterium- mediated transformation of sunflower (helianthus annuus l.)

dc.contributor.authorFraser, Paul David
dc.contributor.authorEnfissi, Eugenia
dc.contributor.authorBramley, Pete
dc.contributor.buuauthorDaǧüstü, Nazan
dc.contributor.departmentZiraat Fakültesi
dc.contributor.departmentTarla Bitkileri Bölümü
dc.contributor.researcheridAAH-1582-2021
dc.contributor.scopusid24480308400
dc.date.accessioned2024-04-04T06:46:35Z
dc.date.available2024-04-04T06:46:35Z
dc.date.issued2008-11
dc.description.abstractThe work described in this paper has used bacterial transgenes (crtI and Hmgr-CoA) which have the potential to increase oil quality in sunflower (Helianthus annuus L.) if an efficient transformation procedure was in place. Optimized procedures for the callus induction from hypocotyl and cotyledon explants, regeneration capacity of sunflower genotypes and transformation to intact embryogenic axis have been established in order to facilitate studies of transformation and production of genetic variability Callus formation was induced easily from hypocotyl and cotyledon explants. Although cotyledon explants produced low amount of callus per explant, the somatic embryo and direct shoot regeneration capacity of cotyledons were generally much higher than that experienced with hypocotyl explants. Only root regeneration was obtained from hypocotyl explants. Regeneration of embryo and shoot varied from 0 - 29% depended on the genotype and explant. For transformation of sunflower, intact embryogenic axis were dissected from seeds and cocultivated with Agrobacterium tumefaciens. Transgenic sunflower lines expressing either the Erwinia uredovora phytoene desaturase (crtI) gene or hydroxymethylglutaryl-CoA (Hmgr-CoA) reductase genes have been obtained Possible transformants were selected by their ability to grow on kanamycin. Transformation was confirmed by PCR and nptII Green shoots were transferred to rooting medium and showed early flowering in in vitro culture efficient (90-100%) than previous reports and has shown in incorporation-a ion of conditions. The transformation system is more efficient effector transgenes.
dc.identifier.citationDağüstü, N. vd. (2008). "Screening for high callus induction and agrobacterium- mediated transformation of sunflower (helianthus annuus l.)". Biotechnology and Biotechnological Equipment, 22(4), 933-937.
dc.identifier.doihttps://doi.org/10.1080/13102818.2008.10817582
dc.identifier.eissn1314-3530
dc.identifier.endpage937
dc.identifier.issn310-2818
dc.identifier.issue4
dc.identifier.scopus2-s2.0-57749172093
dc.identifier.startpage933
dc.identifier.urihttps://www.tandfonline.com/doi/abs/10.1080/13102818.2008.10817582
dc.identifier.urihttps://hdl.handle.net/11452/40981
dc.identifier.volume22
dc.identifier.wos000261341400009
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherTaylor & Francis
dc.relation.collaborationYurt dışı
dc.relation.journalBiotechnology and Biotechnological Equipment
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiotechnology & applied microbiology
dc.subjectCallus production
dc.subjectHelianthus annuusl
dc.subjectSunflower
dc.subjectTransformation
dc.subjectPlant-regeneration
dc.subjectShoot regeneration
dc.subjectHigh-frequency
dc.subjectCotyledons
dc.subjectOrganogenesis
dc.subjectExpression
dc.subjectGenotype
dc.subjectGrowth
dc.subjectTissue
dc.subjectGene
dc.subjectAgrobacterium
dc.subjectAgrobacterium tumefaciens
dc.subjectBacteria (microorganisms)
dc.subjectHelianthus
dc.subjectHelianthus annuus
dc.subjectRhizobium
dc.subject.scopusPlasmopara Halstedii; Helianthus Annuus; Downy Mildew
dc.subject.wosBiotechnology & applied microbiology
dc.titleScreening for high callus induction and agrobacterium- mediated transformation of sunflower (helianthus annuus l.)
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentZiraat Fakültesi/Tarla Bitkileri Bölümü
local.indexed.atWOS
local.indexed.atScopus

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