Development of maize (Zea mays L.) genotypes by using in vivo doubled haploid technique

Abstract

Doubled haploid technology has become an increasingly popular and important tool for developing new maize (Zea mays L.) lines breeding. Although conventional plant breeding contributes significantly to maize development, it is time-consuming and has disadvantages such as using more labor and financial resources. Conventional maize breeding requires repeated self-pollination for about six to ten generations to obtain homozygous inbred lines, also 100% homozygous lines cannot be obtained at the end of this period. Completely homozygous lines can be produced in only two-three generations by using doubled haploid technology. In vivo induction of the maternal haploid method was used for the production of doubled haploid lines. Thirty-two different donors pollinated with haploid inducer line Stock6. Haploid seeds were identified visually by using dominant anthocyanin color marker genes R1-nj. Haploid induction rates (HIR) and chromosome doubling rate (CDR) were determined. Four hundred and eighty eight putative haploid seeds were obtained. The average haploid induction rate was calculated as 2.0%, the average chromosome doubling rate was 52.3%. Results of this study 20 doubled haploid lines were developed.