Publication: Mobile resistance determinants, plasmid replicon types and phylogeny among Escherichia coli strains isolated from cats and dogsom cats and dogs
dc.contributor.author | Kalaycı-Yüksek, F. | |
dc.contributor.author | Gümüş, D. | |
dc.contributor.author | Eroğlu, E. | |
dc.contributor.author | Camadan, D. | |
dc.contributor.author | Ang-Küçüker, M. | |
dc.contributor.buuauthor | Macunluoglu, A. C. | |
dc.contributor.department | Tıp Fakültesi | |
dc.contributor.department | Temel Tıp Bilimleri Ana Bilim Dalı | |
dc.contributor.orcid | 0000-0002-3099-3432 | |
dc.contributor.orcid | 0000-0002-6802-5998 | |
dc.contributor.researcherid | HOH-7979-2023 | |
dc.date.accessioned | 2024-09-12T13:05:33Z | |
dc.date.available | 2024-09-12T13:05:33Z | |
dc.date.issued | 2022-10-01 | |
dc.description.abstract | Multidrug resistance is a great challenge for the treatment of infectious diseases. We determined an-tibiotic resistance patterns, integrons, plasmid-mediated ESBL-, AmpC beta-lactamase-, carbapenemase-, colistin re-sistance genes, plasmid replicon types and phylogeny of fecal Escherichia coli strains isolated from domestic cats and dogs in Turkey. A total of 104 fecal samples of healthy 49 cats and 55 dogs were examined. The integrons, plasmid-mediated resis-tance genes, plasmid replicon types and phylogroups were determined by PCR. Antimicrobial susceptibilities were performed by disc diffusion and microdilution methods. Escherichia coli strains were mostly resistant to AMP (56.73%), SXT (39.42%), CTX (38.46%) and CIP (30.77%). Colistin resistance was not detected. ESBL and carbapenemase rates were 35.5 % and 7.69%, respectively. Eighty (76.9%) and 49 (47.1%) strains were harboring class I and class II integrons, respectively. Besides 12 strains were shown to possess class III integrons. The most frequently detected genes were blaCTX-M (48.08%), blaTEM(45.19%) and blaVIM(20.19%). In our study, none of strains were positive for mcr-1 and mcr-2 genes. Integrons were mostly found on plasmids of incompatibility groups IncF (71.25%) and strains bearing blaCTX-M and blaTEM carried a wide range of plasmid replicons of which IncF, IncFIB, IncK, and IncN. The majority of the strains were grouped in B2 (31.73%) and B1 (22.12%) and resistant bacteria mostly belonged to phylogroup B2. We showed an increasing trend in ESBL-producing E. coli among fecal microbiota members. E. coli strains with dif-ferent plasmid replicon types and phylogroups isolated from cats and dogs can be resistant to various antibiotics which are used in human and veterinary medicine. | |
dc.description.sponsorship | İstanbul Yeni Yüzyıl Üniversitesi BAP 2019/01 | |
dc.identifier.doi | 10.12681/jhvms.30148 | |
dc.identifier.endpage | 5052 | |
dc.identifier.issn | 1792-2720 | |
dc.identifier.issue | 4 | |
dc.identifier.scopus | 2-s2.0-85148207205 | |
dc.identifier.startpage | 5039 | |
dc.identifier.uri | https://doi.org/10.12681/jhvms.30148 | |
dc.identifier.uri | https://hdl.handle.net/11452/44669 | |
dc.identifier.volume | 73 | |
dc.identifier.wos | 000992514100013 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Hellenic Veterinary Medical Soc | |
dc.relation.journal | Journal Of The Hellenic Veterinary Medical Society | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Spectrum-beta-lactamase | |
dc.subject | Antimicrobial resistance | |
dc.subject | Companion animals | |
dc.subject | Molecular characterization | |
dc.subject | Antibiotic-resistance | |
dc.subject | Healthy dogs | |
dc.subject | 2 integrons | |
dc.subject | Genes | |
dc.subject | Prevalence | |
dc.subject | Clone | |
dc.subject | E | |
dc.subject | Coli | |
dc.subject | Integrons | |
dc.subject | Plasmid-mediated resistance genes | |
dc.subject | Plasmid types | |
dc.subject | Phylogeny | |
dc.subject | Science & technology | |
dc.subject | Life sciences & biomedicine | |
dc.subject | Veterinary sciences | |
dc.title | Mobile resistance determinants, plasmid replicon types and phylogeny among Escherichia coli strains isolated from cats and dogsom cats and dogs | |
dc.type | Article | |
dspace.entity.type | Publication | |
local.contributor.department | Tıp Fakültesi/Temel Tıp Bilimleri Ana Bilim Dalı | |
local.indexed.at | WOS | |
local.indexed.at | Scopus |