Publication:
Mobile resistance determinants, plasmid replicon types and phylogeny among Escherichia coli strains isolated from cats and dogsom cats and dogs

dc.contributor.authorKalaycı-Yüksek, F.
dc.contributor.authorGümüş, D.
dc.contributor.authorEroğlu, E.
dc.contributor.authorCamadan, D.
dc.contributor.authorAng-Küçüker, M.
dc.contributor.buuauthorMacunluoglu, A. C.
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentTemel Tıp Bilimleri Ana Bilim Dalı
dc.contributor.orcid0000-0002-3099-3432
dc.contributor.orcid0000-0002-6802-5998
dc.contributor.researcheridHOH-7979-2023
dc.date.accessioned2024-09-12T13:05:33Z
dc.date.available2024-09-12T13:05:33Z
dc.date.issued2022-10-01
dc.description.abstractMultidrug resistance is a great challenge for the treatment of infectious diseases. We determined an-tibiotic resistance patterns, integrons, plasmid-mediated ESBL-, AmpC beta-lactamase-, carbapenemase-, colistin re-sistance genes, plasmid replicon types and phylogeny of fecal Escherichia coli strains isolated from domestic cats and dogs in Turkey. A total of 104 fecal samples of healthy 49 cats and 55 dogs were examined. The integrons, plasmid-mediated resis-tance genes, plasmid replicon types and phylogroups were determined by PCR. Antimicrobial susceptibilities were performed by disc diffusion and microdilution methods. Escherichia coli strains were mostly resistant to AMP (56.73%), SXT (39.42%), CTX (38.46%) and CIP (30.77%). Colistin resistance was not detected. ESBL and carbapenemase rates were 35.5 % and 7.69%, respectively. Eighty (76.9%) and 49 (47.1%) strains were harboring class I and class II integrons, respectively. Besides 12 strains were shown to possess class III integrons. The most frequently detected genes were blaCTX-M (48.08%), blaTEM(45.19%) and blaVIM(20.19%). In our study, none of strains were positive for mcr-1 and mcr-2 genes. Integrons were mostly found on plasmids of incompatibility groups IncF (71.25%) and strains bearing blaCTX-M and blaTEM carried a wide range of plasmid replicons of which IncF, IncFIB, IncK, and IncN. The majority of the strains were grouped in B2 (31.73%) and B1 (22.12%) and resistant bacteria mostly belonged to phylogroup B2. We showed an increasing trend in ESBL-producing E. coli among fecal microbiota members. E. coli strains with dif-ferent plasmid replicon types and phylogroups isolated from cats and dogs can be resistant to various antibiotics which are used in human and veterinary medicine.
dc.description.sponsorshipİstanbul Yeni Yüzyıl Üniversitesi BAP 2019/01
dc.identifier.doi10.12681/jhvms.30148
dc.identifier.endpage5052
dc.identifier.issn1792-2720
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85148207205
dc.identifier.startpage5039
dc.identifier.urihttps://doi.org/10.12681/jhvms.30148
dc.identifier.urihttps://hdl.handle.net/11452/44669
dc.identifier.volume73
dc.identifier.wos000992514100013
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherHellenic Veterinary Medical Soc
dc.relation.journalJournal Of The Hellenic Veterinary Medical Society
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSpectrum-beta-lactamase
dc.subjectAntimicrobial resistance
dc.subjectCompanion animals
dc.subjectMolecular characterization
dc.subjectAntibiotic-resistance
dc.subjectHealthy dogs
dc.subject2 integrons
dc.subjectGenes
dc.subjectPrevalence
dc.subjectClone
dc.subjectE
dc.subjectColi
dc.subjectIntegrons
dc.subjectPlasmid-mediated resistance genes
dc.subjectPlasmid types
dc.subjectPhylogeny
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectVeterinary sciences
dc.titleMobile resistance determinants, plasmid replicon types and phylogeny among Escherichia coli strains isolated from cats and dogsom cats and dogs
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Temel Tıp Bilimleri Ana Bilim Dalı
local.indexed.atWOS
local.indexed.atScopus

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