Publication:
Screening garlic EST sequences for SSR motifs

dc.contributor.authorOnus, A. N.
dc.contributor.buuauthorİpek, Meryem
dc.contributor.buuauthorİpek, Ahmet
dc.contributor.departmentZiraat Fakültesi
dc.contributor.departmentBahçe Bitkileri Bölümü
dc.contributor.researcheridAAH-3233-2021
dc.contributor.researcheridAAK-4655-2021
dc.contributor.scopusid16031208900
dc.contributor.scopusid6603912487
dc.date.accessioned2023-09-08T13:08:00Z
dc.date.available2023-09-08T13:08:00Z
dc.date.issued2016
dc.descriptionBu çalışma, 29 Nisan-02 Mayıs 2012 tarihlerinde Antalya[Türkiye]’ de düzenlenen International Symposium on Biotechnology and other Omics in Vegetable Science Kongresi‘nde bildiri olarak sunulmuştur.
dc.description.abstractSSR markers are one of the most preferable DNA molecular markers in plant genetic analyses because they are multi allelic, polymorphic, reproducible and transferable among the genetically close species although their development is costly and time consuming. Development of SSR markers can be accomplished with either sequencing of genomic library colonies containing SSR repeats or mining available sequences of related species in gene banks. The second method has been preferred in recent studies due to the dramatic increase in available nucleotide sequences of plant species. For garlic genome, limited SSR markers have been developed so far and development of more SSR markers is needed for detailed genetic characterization and mapping of garlic genotypes. In this study, we screened available garlic EST sequences for SSR motifs in order to generate EST-based SSR markers. We searched repeat motifs containing 2-6 nucleotides. The minimum repeat units criteria was ten for dinucleotides, seven for trinucleotides, five for tetranucleotides, and four for pentanucleotides and hexanucleotides. In total, 86 SSR motifs have been determined using these criteria and the most abounded repeat motifs were determined as (AT/TA)n. Primer pairs designed for some of these SSR motifs were observed as multi allelic and polymorphic among the garlic clones.
dc.description.sponsorshipInt Soc Hort Sci
dc.identifier.citationİpek, M. ve İpek, A. (2016). "Screening garlic EST sequences for SSR motifs". ed. A. N. Onus. Acta Horticulturae, International Symposium on Biotechnology and Other Omics in Vegetable Science, 1145, 101-104.
dc.identifier.endpage104
dc.identifier.isbn978-94-62611-34-4
dc.identifier.issn0567-7572
dc.identifier.issn2406-6168
dc.identifier.scopus2-s2.0-85007496868
dc.identifier.startpage101
dc.identifier.urihttps://doi.org/10.17660/ActaHortic.2016.1145.16
dc.identifier.urihttps://www.actahort.org/books/1145/1145_16.htm
dc.identifier.urihttp://hdl.handle.net/11452/33807
dc.identifier.volume1145
dc.identifier.wos000392632200016
dc.indexed.wosCPCIS
dc.language.isoen
dc.publisherInternational Society for Horticultural Science
dc.relation.bapZ-2010/50
dc.relation.journalActa Horticulturae, International Symposium on Biotechnology and Other Omics in Vegetable Science
dc.relation.publicationcategoryKonferans Öğesi – Uluslararası
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiotechnology & applied microbiology
dc.subjectPlant sciences
dc.subjectAgriculture
dc.subjectAllium sativum
dc.subjectExpressed sequence tag
dc.subjectSimple sequence repeats
dc.subjectGenome
dc.subjectGenetic diversity
dc.subjectMarkers
dc.subjectClones
dc.subject.scopusBulbs; Allium Roseum; Bolting
dc.subject.wosBiotechnology & applied microbiology
dc.subject.wosPlant sciences
dc.subject.wosHorticulture
dc.titleScreening garlic EST sequences for SSR motifs
dc.typeProceedings Paper
dspace.entity.typePublication
local.contributor.departmentZiraat Fakültesi/Bahçe Bitkileri Bölümü
local.indexed.atScopus
local.indexed.atWOS

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