Publication:
Real-time polymerase chain reaction for detection of mycoplasma gallisepticum in chicken trachea

dc.contributor.buuauthorÇarlı, Kamil Tayfun
dc.contributor.buuauthorEyigör, Ayşegül
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.departmentMikrobiyoloji Ana Bilim Dalı
dc.contributor.departmentBesin Hijyeni ve Teknolojisi Ana Bilim Dalı
dc.contributor.researcheridAAI-1101-2021
dc.contributor.scopusid6601971539
dc.contributor.scopusid6602558950
dc.date.accessioned2021-10-07T12:29:55Z
dc.date.available2021-10-07T12:29:55Z
dc.date.issued2003-07
dc.description.abstractIn this work, we describe a rapid detection procedure for Mycoplasma gallisepticum from chicken tracheal swabs by real-time polymerase chain reaction (PCR) by LightCycler system, where we were able to monitor the amplification of the newly synthesized M gallisepticum-specific PCR product as a proportionally increasing fluorescent signal by using the double-stranded DNA binding dye SYBR Green I and have identified M. gallisepticum-specific PCR products by DNA melting curve analysis by plotting the first negative derivative (-d[F1]/dT) of fluorescence over temperature. Detection limits of the PCR were found to be 3 and 3000 colony-forming units ml(-1) with pure culture of M. gallisepticum and artificially spiked samples, respectively. Out of 96 tracheal swabs, 68 were taken from live chickens and 28 were taken by scraping the mucosal surface of the trachea (SMST) of necropsied chickens. All of the 18 PCR-positive results were from the swabs taken by the SMST method, whereas all of the samples taken from live chickens were negative. Thus, the PCR with the SMST method had a sensitivity and a specificity of 64.2% (18 of 28 chickens) and 100%, respectively. The total time required for template preparation from tracheal swab samples and real-time PCR was approximately 65 min. These results indicate that real-time PCR with the LightCycler technology is a rapid and sensitive test to identify M. gallisepticum-infected flocks if a proper sampling is applied.
dc.identifier.citationÇarlı, K.T. ve Eyigör, A. (2003). “Real-time polymerase chain reaction for detection of mycoplasma gallisepticum in chicken trachea”. Avian Diseases, 47(3), 712-717.
dc.identifier.endpage717
dc.identifier.issn0005-2086
dc.identifier.issue3
dc.identifier.pubmed14562901
dc.identifier.scopus2-s2.0-0141682982
dc.identifier.startpage712
dc.identifier.urihttps://doi.org/10.1637/6041
dc.identifier.urihttp://hdl.handle.net/11452/22285
dc.identifier.volume47
dc.identifier.wos000185415100025
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherAmer Assoc Avian Pathologists
dc.relation.journalAvian Diseases
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectVeterinary sciences
dc.subjectMycoplasma gallisepticum
dc.subjectPoultry
dc.subjectReal-time PCR
dc.subjectAves
dc.subjectBacteria (microorganisms)
dc.subjectGallus gallus
dc.subjectMycoplasma
dc.subjectTetrathionate broth enrichment
dc.subjectCapillary PCR
dc.subjectDiagnosis
dc.subjectSynoviae
dc.subjectDNA
dc.subject.scopusMycoplasma Synoviae; Carpodacus Mexicanus; Poultry Industry
dc.subject.wosVeterinary sciences
dc.titleReal-time polymerase chain reaction for detection of mycoplasma gallisepticum in chicken trachea
dc.typeArticle
dc.wos.quartileQ2
dspace.entity.typePublication
local.contributor.departmentVeteriner Fakültesi/Besin Hijyeni ve Teknolojisi Ana Bilim Dalı
local.contributor.departmentVeteriner Fakültesi/Mikrobiyoloji Ana Bilim Dalı
local.indexed.atPubMed
local.indexed.atWOS

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