Publication:
Carbapenem and colistin resistance, integrons and plasmid replicon types in multi-drug resistant klebsiella strains isolated in Turkey

Placeholder

Organizational Units

Authors

Macunluoğlu, Aslı-Ceren

Authors

Kalaycı-Yüksek, Fatma
Gümüş, Defne
Uyanık-Öçal, Aysun
Gün, Gökhan
Bayırlı-Turan, Derya
Macunluoğlu, Aslı-Ceren
Nakipoğlu, Yaşar
Ang-Küçükker, Mine

Advisor

Language

Publisher:

Clin Lab Publ

Journal Title

Journal ISSN

Volume Title

Abstract

Background: Multidrug-resistant (MDR) Klebsiella is a globally important nosocomial pathogen. In the present study, 101 multidrug-resistant Klebsiella strains isolated from various clinical specimens obtained from two differ-ent Medical Faculties' hospitals were involved. We aimed to find out the prevalence of carbapenemase-, mobile colistin resistance genes, and integrons in MDR Klebsiella strains.Methods: The antibiotic susceptibilities of strains were determined by Kirby Bauer disc-diffusion method and re-sistance to colistin was confirmed by detection of minimum inhibitory concentrations. The prevalence of carba-penemase genes (blaOXA-48 , blaNDM , blaIMP , blaVIM , blaKPC), mobile colistin-resistance genes (mcr-1 and mcr-2), and integrons (class I, II and III) were examined in Klebsiella strains by polymerase chain reaction.Results: All strains were resistant to beta-lactam antibiotics, carbapenems, and quinolones. On the other hand, only nine (8.9%) strains were resistant to colistin. The most common carbapenemase genes were blaNDM (64.3%) and blaOXA-48 (53.5%). Besides, 28 (27.7%) strains were found to harbor both blaNDM and blaOXA-48. These 28 strains be-longed to the IncA/C (18.7%), IncL/M (7.7%), and IncFIIs (1.1%) plasmid replicon types. No strain was positive for blaIMP , mcr-1 , and mcr-2. Class I and Class II integrons were shown to be harbored in 83.2% and 63.3% of strains, respectively. In total, 63 (63.6%) of strains harbored both classes I and II integrons. Class III integron was not detected. There was a statistically significant relationship between the presence of integrons and antibiotic re-sistance for cefotaxime (p = 0.024), ciprofloxacin (p < 0.001) trimethoprim/sulfamethoxazole (p < 0.001) and levo-floxacin (p = 0.002). To our knowledge, this study represents the first report of a human isolate for the co-presence of blaNDM , blaOXA-48 and both Class I and Class II integrons, from Turkey.Conclusions: Our findings also highlight the dissemination of integrons and carbapenemases and the importance of surveillance on emerging antibiotic resistance.

Description

Source:

Keywords:

Keywords

Spectrum beta-lactamase, Gram-negative bacteria, Molecular characterization, Escherichia-coli, Pneumoniae strains, Gene, Enterobacteriaceae, Identification, Spread, Carbapenem resistance, Colistin resistance, Mdr klebsi-ella strains, Integrons, Plasmid replicon types, Science & technology, Life sciences & biomedicine, Medical laboratory technology

Citation

Endorsement

Review

Supplemented By

Referenced By

2

Views

0

Downloads

View PlumX Details