Publication:
Salmonella carriage and change in serovar distribution in broiler giblets at slaughterhouse level in turkiye: First report using iso 6579-1: 2017 and iso 6579-3: 2014

dc.contributor.authorÇetin, Ece
dc.contributor.buuauthorEyigor, A.
dc.contributor.buuauthorEYİGÖR, AYŞE GÜL
dc.contributor.buuauthorTemelli, Süreyya
dc.contributor.buuauthorCoşkun, Ahmet Gökhan
dc.contributor.buuauthorAkgün, Zeynep
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.departmentBesin Hijyeni ve Teknolojisi Ana Bilim Dalı.
dc.contributor.orcid0000-0002-2707-3117
dc.contributor.researcheridLVS-7644-2024
dc.contributor.researcheridAAI-1101-2021
dc.date.accessioned2025-02-05T06:16:43Z
dc.date.available2025-02-05T06:16:43Z
dc.date.issued2024-05-14
dc.description.abstractThis study aimed to determine the prevalence and serovar distribution of salmonellae in liver, heart, and spleen ( LHS ) and gizzard ( G ) of slaughtered broilers. For this, a total of 60 sample units, comprised of 30 LHS and 30 G collected from 3 slaughterhouses, were analysed by reference methods for detection and serotyping as revised ISO 6579-1:2017 and ISO 6579-3:2014, respectively. Also, Salmonellaspecific real-time PCR ( Salm-PCR ) was used for species confirmation, while Salmonella Enteritidis ( S . Enteritidis ) and Salmonella Typhimurium ( S . Typhimurium ) specific real-time PCR (SE/ST-PCR) was evaluated to determine its efficiency for rapid detection of the serovars mandated in current legal regulations compared to standard serotyping. All LHS (100% -30/30) and 90% (27/30) of G samples harbored Salmonella with an overall prevalence of 95% (57/60) in samples examined, where all isolates were confirmed as Salmonella by Salm-PCR. The most prevalent serovar in broiler giblets was S. Virchow (80.70% -46/57) followed by S. Enteritidis (19.30% -11/57). SE/STPCR (%17.54 -10/57) could not detect one G isolate, which was serotyped as S. Enteritidis by standard serotyping. High relative accuracy (98.25%), sensitivity (100%) and specificity (100%), and agreement between methods ( K : 0.94) verified SE/ST-PCR 's potential to be used as an alternative in rapid detection of S . Enteritidis and S . Typhimurium. Data on high Salmonella prevalence in broiler giblets of slaughterhouse origin, and detection of the pathogen by the implementation of all requirements indicated in the revised ISO 65791:2017 standard method, enabling the determination of actual prevalence in the samples with high sensitivity and specificity is of significance for public health. Additionally, identification of S . Virchow as the dominant serovar followed by S . Enteritidis with a relatively lower prevalence, and absence of S. Typhimurium in broiler giblets are important findings for Turkiye. This up to date data, obtained by strict application of ISO 6579-3:2014 procedures, indicated a shift in circulating serovars in the broiler industry. The objective findings in this study would bring awareness to national/international literature, and may be of use in future improvements in legal regulations.
dc.identifier.doi10.1016/j.psj.2024.103805
dc.identifier.issn0032-5791
dc.identifier.issue7
dc.identifier.scopus2-s2.0-85192799010
dc.identifier.urihttps://doi.org/10.1016/j.psj.2024.103805
dc.identifier.urihttps://hdl.handle.net/11452/50085
dc.identifier.volume103
dc.identifier.wos001242701900001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherElsevier
dc.relation.bapTGA-2021-488
dc.relation.journalPoultry Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntibiotic-resistance
dc.subjectChicken meat
dc.subjectPrevalence
dc.subjectEnterica
dc.subjectLiver
dc.subjectSalmonella
dc.subjectBroiler
dc.subjectGiblet
dc.subjectIso 6579-1:2017
dc.subjectIso 6579-3:2014
dc.subjectReal-time pcr
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectAgriculture, dairy & animal science
dc.subjectAgriculture
dc.titleSalmonella carriage and change in serovar distribution in broiler giblets at slaughterhouse level in turkiye: First report using iso 6579-1: 2017 and iso 6579-3: 2014
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentVeteriner Fakültesi/Besin Hijyeni ve Teknolojisi Ana Bilim Dalı.
local.indexed.atWOS
local.indexed.atScopus
relation.isAuthorOfPublicationa7cf195c-b259-4e7e-a137-d482249fa08f
relation.isAuthorOfPublication.latestForDiscoverya7cf195c-b259-4e7e-a137-d482249fa08f

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Akgun_vd_2024.pdf
Size:
305.18 KB
Format:
Adobe Portable Document Format