Publication: Salmonella carriage and change in serovar distribution in broiler giblets at slaughterhouse level in turkiye: First report using iso 6579-1: 2017 and iso 6579-3: 2014
dc.contributor.author | Çetin, Ece | |
dc.contributor.buuauthor | Eyigor, A. | |
dc.contributor.buuauthor | EYİGÖR, AYŞE GÜL | |
dc.contributor.buuauthor | Temelli, Süreyya | |
dc.contributor.buuauthor | Coşkun, Ahmet Gökhan | |
dc.contributor.buuauthor | Akgün, Zeynep | |
dc.contributor.department | Veteriner Fakültesi | |
dc.contributor.department | Besin Hijyeni ve Teknolojisi Ana Bilim Dalı. | |
dc.contributor.orcid | 0000-0002-2707-3117 | |
dc.contributor.researcherid | LVS-7644-2024 | |
dc.contributor.researcherid | AAI-1101-2021 | |
dc.date.accessioned | 2025-02-05T06:16:43Z | |
dc.date.available | 2025-02-05T06:16:43Z | |
dc.date.issued | 2024-05-14 | |
dc.description.abstract | This study aimed to determine the prevalence and serovar distribution of salmonellae in liver, heart, and spleen ( LHS ) and gizzard ( G ) of slaughtered broilers. For this, a total of 60 sample units, comprised of 30 LHS and 30 G collected from 3 slaughterhouses, were analysed by reference methods for detection and serotyping as revised ISO 6579-1:2017 and ISO 6579-3:2014, respectively. Also, Salmonellaspecific real-time PCR ( Salm-PCR ) was used for species confirmation, while Salmonella Enteritidis ( S . Enteritidis ) and Salmonella Typhimurium ( S . Typhimurium ) specific real-time PCR (SE/ST-PCR) was evaluated to determine its efficiency for rapid detection of the serovars mandated in current legal regulations compared to standard serotyping. All LHS (100% -30/30) and 90% (27/30) of G samples harbored Salmonella with an overall prevalence of 95% (57/60) in samples examined, where all isolates were confirmed as Salmonella by Salm-PCR. The most prevalent serovar in broiler giblets was S. Virchow (80.70% -46/57) followed by S. Enteritidis (19.30% -11/57). SE/STPCR (%17.54 -10/57) could not detect one G isolate, which was serotyped as S. Enteritidis by standard serotyping. High relative accuracy (98.25%), sensitivity (100%) and specificity (100%), and agreement between methods ( K : 0.94) verified SE/ST-PCR 's potential to be used as an alternative in rapid detection of S . Enteritidis and S . Typhimurium. Data on high Salmonella prevalence in broiler giblets of slaughterhouse origin, and detection of the pathogen by the implementation of all requirements indicated in the revised ISO 65791:2017 standard method, enabling the determination of actual prevalence in the samples with high sensitivity and specificity is of significance for public health. Additionally, identification of S . Virchow as the dominant serovar followed by S . Enteritidis with a relatively lower prevalence, and absence of S. Typhimurium in broiler giblets are important findings for Turkiye. This up to date data, obtained by strict application of ISO 6579-3:2014 procedures, indicated a shift in circulating serovars in the broiler industry. The objective findings in this study would bring awareness to national/international literature, and may be of use in future improvements in legal regulations. | |
dc.identifier.doi | 10.1016/j.psj.2024.103805 | |
dc.identifier.issn | 0032-5791 | |
dc.identifier.issue | 7 | |
dc.identifier.scopus | 2-s2.0-85192799010 | |
dc.identifier.uri | https://doi.org/10.1016/j.psj.2024.103805 | |
dc.identifier.uri | https://hdl.handle.net/11452/50085 | |
dc.identifier.volume | 103 | |
dc.identifier.wos | 001242701900001 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Elsevier | |
dc.relation.bap | TGA-2021-488 | |
dc.relation.journal | Poultry Science | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Antibiotic-resistance | |
dc.subject | Chicken meat | |
dc.subject | Prevalence | |
dc.subject | Enterica | |
dc.subject | Liver | |
dc.subject | Salmonella | |
dc.subject | Broiler | |
dc.subject | Giblet | |
dc.subject | Iso 6579-1:2017 | |
dc.subject | Iso 6579-3:2014 | |
dc.subject | Real-time pcr | |
dc.subject | Science & technology | |
dc.subject | Life sciences & biomedicine | |
dc.subject | Agriculture, dairy & animal science | |
dc.subject | Agriculture | |
dc.title | Salmonella carriage and change in serovar distribution in broiler giblets at slaughterhouse level in turkiye: First report using iso 6579-1: 2017 and iso 6579-3: 2014 | |
dc.type | Article | |
dspace.entity.type | Publication | |
local.contributor.department | Veteriner Fakültesi/Besin Hijyeni ve Teknolojisi Ana Bilim Dalı. | |
local.indexed.at | WOS | |
local.indexed.at | Scopus | |
relation.isAuthorOfPublication | a7cf195c-b259-4e7e-a137-d482249fa08f | |
relation.isAuthorOfPublication.latestForDiscovery | a7cf195c-b259-4e7e-a137-d482249fa08f |
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