Publication:
Characterisation of genital mycoplasma species from preputial swabs of bucks and rams

dc.contributor.authorGöçmen, Huban
dc.contributor.authorRosales, Ruben S.
dc.contributor.authorRidley, Anne
dc.contributor.buuauthorAlcay, Selim
dc.contributor.buuauthorALÇAY, SELİM
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.researcheridJJQ-9411-2023
dc.date.accessioned2024-07-09T10:58:41Z
dc.date.available2024-07-09T10:58:41Z
dc.date.issued2020-03-01
dc.description.abstractMycoplasma species are a major cause of mastitis, arthritis, pneumonia and reproductive disorders in small ruminants. Mycoplasmas in reproductive systems of males have been associated with diseases as orchitis, balanoposthitis and abnormal spermatozoa activity. The aim of this study was to detect Mycoplasma species that cause reproductive infections in bucks and rams. Total of 27 preputial swabs was collected from Saanen bucks and Kivircik rams at the Artificial Insemination (Al) Center of Uludag University in Turkey. Bacteriological culture methods, followed by Polymerase Chain Reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) were used to detect and identify Mycoplasma species. The PCR-DGGE method identified one M. bovigenitalium and one M. arginini from two cases of orchitis in rams, and another M. bovigenitalium was identified from a buck with no clinical signs. The results showed that Mycoplasma species were present in the testicles of rams and bucks that were negative for Brucellosis, and likely causative organisms of orchitis which lead to reduced fertility.
dc.identifier.doi10.9775/kvfd.2019.22890
dc.identifier.endpage308
dc.identifier.issn1300-6045
dc.identifier.issue2
dc.identifier.startpage305
dc.identifier.urihttps://doi.org/10.9775/kvfd.2019.22890
dc.identifier.urihttps://hdl.handle.net/11452/43080
dc.identifier.volume26
dc.identifier.wos000508967700021
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisi
dc.relation.journalKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSheep
dc.subjectPcr
dc.subjectGenital mycoplasmas
dc.subjectPcr-dgge
dc.subjectPreputial swab
dc.subjectSmall ruminant
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectVeterinary sciences
dc.titleCharacterisation of genital mycoplasma species from preputial swabs of bucks and rams
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentVeteriner Fakültesi
relation.isAuthorOfPublication2f32ebee-28b4-4e2e-8f35-bcaf3ecf5ff1
relation.isAuthorOfPublication.latestForDiscovery2f32ebee-28b4-4e2e-8f35-bcaf3ecf5ff1

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