Publication:
P-glycoprotein expression by technetium-99m-MIBI scintigraphy in hematologic malignancy

dc.contributor.authorKostakoğlu, Lale
dc.contributor.authorDicle, Güç
dc.contributor.authorCanpınar, Hande
dc.contributor.authorKars, Ayşe
dc.contributor.authorKıratlı, Pınar
dc.contributor.authorHayran, Mutlu
dc.contributor.authorGündüz, Ufuk
dc.contributor.authorKansu, Emin
dc.contributor.buuauthorAlper, Eray
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentNükleer Tıp Ana Bilim Dalı
dc.date.accessioned2021-06-30T11:29:42Z
dc.date.available2021-06-30T11:29:42Z
dc.date.issued1998
dc.description.abstractOur aim was to ascertain the relationship between the degree of Tc-99m-MIBI uptake and the level of p-glycoprotein (Pgp) expression determined by flow cytometry and reverse transcription-polymerase chain reaction (RT-PCR) techniques in patients with hematologic malignancy. Methods: A total of 21 samples (19 patients) were evaluated. Two patients had repeat studies after therapy. Thirteen samples were studied at the time of initial diagnosis and 8 during relapse after therapy. After MIBI imaging, either bone marrow aspiration or peripheral blood was obtained for flow cytometric and RT-PCR analyses, Flow cytometry was performed using two different antibodies. After the injection of 555 MBq MIBI, whole-body and pelvic spot images were acquired using a dual-head gamma camera, The uptake in the bone marrow was evaluated against the background (adjacent soft tissue) by both qualitative (scoring system) and quantitative (tm/bkg ratios) analyses, Results: For flow cytometry, the limit for Pgp overexpression was set at >15% Pgp-positive mononuclear bone marrow or peripheral blood cells. There was an inverse correlation between the levels of Pgp and MIBI imaging using both the qualitative (scoring system) and quantitative (tm/bkg ratios) analyses (p = 0.022), Mean values were statistically different between Pgp + and Pgp - groups for both qualitative and quantitative analyses (p = 0.009 and 0.024, respectively). For RT-PCR, there was statistical support toward a difference in the mean values between Pgp + and Pgp - groups by qualitative analysis (p = 0.061); however, no statistical difference was found between these two groups by quantitative analysis (p = 0.179), Conclusion: Based on the strong correlation between the imaging and flow cytometry and a statistical support toward the correlation between the imaging and RT-PCR, MIBI imaging may be used for the in vivo detection of Pgp in patients with hematologic malignancy.
dc.identifier.citationKostakoğlu, L. vd. (1998). "P-glycoprotein expression by technetium-99m-MIBI scintigraphy in hematologic malignancy". Journal of Nuclear Medicine, 39(7), 1191-1197.
dc.identifier.endpage1197
dc.identifier.issn0161-5505
dc.identifier.issue7
dc.identifier.pubmed9669392
dc.identifier.scopus2-s2.0-0031814269
dc.identifier.startpage1191
dc.identifier.urihttps://www.proquest.com/docview/219225468
dc.identifier.urihttps://jnm.snmjournals.org/content/39/7/1191
dc.identifier.urihttp://hdl.handle.net/11452/20939
dc.identifier.volume39
dc.identifier.wos000074691600019
dc.indexed.scopusScopus
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherSoc Nuclear Medicine Inc
dc.relation.collaborationYurt içi
dc.relation.collaborationYurt dışı
dc.relation.journalJournal of Nuclear Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMultidrug resistance
dc.subjectP-glycoprotein
dc.subjectTechnetium-99m-MIBI
dc.subjectReverse transcription-polymerase chain reaction techniques
dc.subjectFlow cytometry
dc.subjectMultidrug-resistance gene
dc.subjectDrug-resistance
dc.subjectHuman-tumors
dc.subjectLeukemia
dc.subjectCells
dc.subjectFlow
dc.subjectMitochondrial
dc.subjectRhodamine-123
dc.subjectAccumulation
dc.subjectTransporter
dc.subjectRadiology, nuclear medicine & medical imaging
dc.subject.wosRadiology, nuclear medicine & medical imaging
dc.titleP-glycoprotein expression by technetium-99m-MIBI scintigraphy in hematologic malignancy
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Nükleer Tıp Ana Bilim Dalı
local.indexed.atWOS
local.indexed.atScopus

Files

License bundle

Now showing 1 - 1 of 1
Placeholder
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: