Publication:
Dna extraction techniques compared for accurate detection of genetically modified organisms (gmos) in maize food and feed products

dc.contributor.authorKazan, Hande
dc.contributor.authorKaracanlı, Burcin
dc.contributor.authorLucas, Stuart J.
dc.contributor.buuauthorTürkeç, Aydın
dc.contributor.buuauthorTÜRKEÇ, AYDIN
dc.contributor.departmentMustafakemalpaşa Meslek Yüksekokulu
dc.contributor.researcheridAAH-6969-2021
dc.date.accessioned2024-08-13T08:52:56Z
dc.date.available2024-08-13T08:52:56Z
dc.date.issued2015-08-01
dc.description.abstractIn this paper, DNA extraction methods have been evaluated to detect the presence of genetically modified organisms (GMOs) in maize food and feed products commercialised in Turkey. All the extraction methods tested performed well for the majority of maize foods and feed products analysed. However, the highest DNA content was achieved by the Wizard, Genespin or the CTAB method, all of which produced optimal DNA yield and purity for different maize food and feed products. The samples were then screened for the presence of GM elements, along with certified reference materials. Of the food and feed samples, 8 % tested positive for the presence of one GM element (NOS terminator), of which half (4 % of the total) also contained a second element (the Cauliflower Mosaic Virus 35S promoter). The results obtained herein clearly demonstrate the presence of GM maize in the Turkish market, and that the Foodproof GMO Screening Kit provides reliable screening of maize food and feed products.
dc.description.sponsorshipTurkiye Cumhuriyeti Kalkinma Bakanlığı Ministry of Science, Industry & Technology - Turkey
dc.description.sponsorshipElips Health Products Ltd.
dc.identifier.doi10.1007/s13197-014-1547-8
dc.identifier.endpage5171
dc.identifier.issn0022-1155
dc.identifier.issue8
dc.identifier.scopus2-s2.0-84938342560
dc.identifier.startpage5164
dc.identifier.urihttps://doi.org/10.1007/s13197-014-1547-8
dc.identifier.urihttps://hdl.handle.net/11452/43969
dc.identifier.volume52
dc.identifier.wos000358665800050
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherSpringer India
dc.relation.journalJournal Of Food Science And Technology-mysore
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi
dc.subjectReal-time pcr
dc.subjectTraceability
dc.subjectSoy
dc.subjectFood
dc.subjectFeed
dc.subjectGm maize
dc.subjectDna extraction
dc.subjectMultiplex real-time pcr screening
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectFood science & technology
dc.titleDna extraction techniques compared for accurate detection of genetically modified organisms (gmos) in maize food and feed products
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentMustafakemalpaşa Meslek Yüksekokulu
local.indexed.atWOS
local.indexed.atScopus
relation.isAuthorOfPublicatione13d661c-dafe-458d-a31b-cfe1dbb7f471
relation.isAuthorOfPublication.latestForDiscoverye13d661c-dafe-458d-a31b-cfe1dbb7f471

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Turkec_vd_2015.pdf
Size:
243.86 KB
Format:
Adobe Portable Document Format