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Rapid, practical and safe isolation of adipose derived stem cells

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dc.contributor.buuauthorÇerçi, Ece
dc.contributor.buuauthorErdost, Hatice
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.departmentHistoloji Embriyoloji Ana Bilim Dalı
dc.contributor.orcid0000-0002-2740-4262
dc.contributor.orcid0000-0003-1547-7293
dc.contributor.researcheridAAH-9216-2021
dc.contributor.scopusid57212212053
dc.contributor.scopusid6505787570
dc.date.accessioned2022-12-08T08:16:26Z
dc.date.available2022-12-08T08:16:26Z
dc.date.issued2020-06-23
dc.description.abstractWe compared migration, proliferation, growth curve, confluency and differentiation into adipogenic, osteogenic and chondrogenic cell lineages of mesenchymal stem cells derived from adipose tissue cultured in scratched and nonscratched cell culture flasks. Mesenchymal stem cells were isolated from rat adipose tissue using a nonenzymatic method. We investigated two groups. For the control group, minced adipose tissue was implanted conventionally onto the surface of standard plastic cell culture flasks. For the experimental group, the tissues were cultured in flasks with a scratched surface. We found that scratched flasks promoted cell migration, proliferation and confluency. Our findings suggest that scratched flasks may be used to ensure rapid, practical and safe isolation of adipose tissue-derived stem cells.
dc.identifier.citationÇerçi, E. ve Erdost, H. (2021). "Rapid, practical and safe isolation of adipose derived stem cells". Biotechnic & Histochemistry, 96(2), 138-145.
dc.identifier.doi10.1080/10520295.2020.1776895
dc.identifier.issn1052-0295
dc.identifier.issn1473-7760
dc.identifier.issue2
dc.identifier.pubmed32573273
dc.identifier.scopus2-s2.0-85087351102
dc.identifier.urihttps://doi.org/10.1080/10520295.2020.1776895
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.1080/10520295.2020.1776895
dc.identifier.urihttp://hdl.handle.net/11452/29748
dc.identifier.volume96
dc.identifier.wos000547775300001
dc.indexed.scopusScopus
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherTaylor & Francis
dc.relation.bapOUAP 2015/25
dc.relation.journalBiotechnic & Histochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAdipose tissue
dc.subjectCulture flasks
dc.subjectIsolation
dc.subjectMesenchymal stem cell
dc.subjectRat
dc.subjectScratched flask
dc.subjectExtracellular-matrix
dc.subjectDifferentiation
dc.subjectTissue
dc.subjectBiotechnology & applied microbiology
dc.subjectCell biology
dc.subject.emtreeAdipocyte
dc.subject.emtreeAdipose tissue
dc.subject.emtreeAnimal
dc.subject.emtreeCell culture
dc.subject.emtreeCell differentiation
dc.subject.emtreeCell proliferation
dc.subject.emtreeRat
dc.subject.emtreeStem cell
dc.subject.meshAdipocytes
dc.subject.meshAdipose tissue
dc.subject.meshAnimals
dc.subject.meshCell differentiation
dc.subject.meshCell proliferation
dc.subject.meshCells, cultured
dc.subject.meshRats
dc.subject.meshStem Cells
dc.subject.scopusStromal Cells; Adipose Tissues; Adipose Derived Stem Cell
dc.subject.wosBiotechnology & applied microbiology
dc.subject.wosCell biology
dc.titleRapid, practical and safe isolation of adipose derived stem cells
dc.typeArticle
dc.wos.quartileQ4
dspace.entity.typePublication
local.contributor.departmentVeteriner Fakültesi/Histoloji Embriyoloji Ana Bilim Dalı
local.indexed.atPubMed
local.indexed.atWOS
local.indexed.atScopus

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