Publication:
A new protocol for collagen-induced local arthritis model in balb/c mice

dc.contributor.buuauthorGüvenç, Gökçen
dc.contributor.buuauthorKaracay, Mehmet
dc.contributor.buuauthorÇiftçi, Kübra
dc.contributor.buuauthorAkkoç, Ahmet
dc.contributor.buuauthorAKKOÇ, AHMET
dc.contributor.buuauthorOral, Haluk Barbaros
dc.contributor.buuauthorORAL, HALUK BARBAROS
dc.contributor.buuauthorYalçın, Murat
dc.contributor.buuauthorYALÇIN, MURAT
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentFizyoloji Ana Bilim Dalı
dc.contributor.departmentPatoloji Ana Bilim Dalı
dc.contributor.departmentİmmunoloji Ana Bilim Dalı
dc.contributor.orcid0000-0002-1413-3651
dc.contributor.orcid0000-0002-5090-7917
dc.contributor.orcid0000-0003-0463-6818
dc.contributor.orcid0000-0002-5600-8162
dc.contributor.researcheridAAR-6815-2021
dc.contributor.researcheridK-7285-2012
dc.contributor.researcheridAAG-6956-2021
dc.date.accessioned2024-10-07T08:41:14Z
dc.date.available2024-10-07T08:41:14Z
dc.date.issued2018-01-01
dc.description.abstractIntroduction: Numerous models have been described to study the pathogenesis of rheumatoid arthritis, and to develop new therapies; but each of these models has their own limitations. Nowadays, the collagen-induced arthritis (CIA) mouse model is the most commonly used model for rheumatoid arthritis studies in certain mouse strain like DBA/1 mouse. This study aimed to describe a new protocol for local induction of arthritis in Balb/c mice, including the monitoring of clinical arthritis and the protocols for histological examination of paws of mice.Materials and Methods: For the induction of local arthritis in 40 Balb/c mice, they were immunized intra-articularly with type II collagen and subcutaneous complete Freund's adjuvant in 0 day. As the boost immunization, the animals had same injections on day 14. Mice were divided 4 groups, and they were clinically and histopathologically examined for arthritis on 0, 14, 21 and 30 days of arthritis induction.Results: The first signs ol local arthritis appeared in this model 1 week after boost immunization (day 21). The CIA induced paw clinically showed severe erythema and swelling all around the hindquarter on day 21 and 30. The paw reached almost 4 times thicker than the other paws and histopathological examination confirmed the clinical arthritis on day 21 and 30.Conclusion: Using the protocol described, the investigators may reproducibly and economically induce a high incidence of local CIA in Balb/c mice. The described local CLA model may be used to unravel pathophysiological or immunological mechanisms of arthritis, and can also be used to study the effect of new therapeutics.
dc.identifier.doi10.25002/tji.2018.771
dc.identifier.endpage36
dc.identifier.issn1301-109X
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85046121443
dc.identifier.startpage30
dc.identifier.urihttps://doi.org/10.25002/tji.2018.771
dc.identifier.urihttps://hdl.handle.net/11452/45954
dc.identifier.volume6
dc.identifier.wos000441388100005
dc.indexed.wosWOS.ESCI
dc.language.isoen
dc.publisherTurkish Soc Immunology
dc.relation.journalTurkish Journal Of Immunology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIi collagen
dc.subjectRheumatoid-arthritis
dc.subjectPhenotype development
dc.subjectAutoimmune arthritis
dc.subjectIdentification
dc.subjectDeterminant
dc.subjectInduction
dc.subjectSusceptibility
dc.subjectImmunization
dc.subjectAntibodies
dc.subjectExperimental model
dc.subjectRheumatoid arthritis
dc.subjectCollagen-induced arthritis
dc.subjectBalb/c mouse
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectImmunology
dc.titleA new protocol for collagen-induced local arthritis model in balb/c mice
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentVeteriner Fakültesi/Fizyoloji Ana Bilim Dalı
local.contributor.departmentVeteriner Fakültesi/Patoloji Ana Bilim Dalı
local.contributor.departmentTıp Fakültesi/İmmunoloji Ana Bilim Dalı
local.indexed.atWOS
local.indexed.atScopus
relation.isAuthorOfPublication77d6f8ff-e2a2-45b4-a991-48f59fee43a4
relation.isAuthorOfPublicationbc853dab-a811-42b9-b03b-6d5fe067efe8
relation.isAuthorOfPublication90abe2d4-19a5-42d8-b921-d41cf6e684c8
relation.isAuthorOfPublication.latestForDiscovery77d6f8ff-e2a2-45b4-a991-48f59fee43a4

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