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Hypovolemic hemorrhage induces Fos expression in the rat hypothalamus: Evidence for involvement of the lateral hypothalamus in the decompensatory phase of hemorrhage

dc.contributor.authorMillington, William R.
dc.contributor.buuauthorGöktalay, Gökhan
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentTıbbi Farmakoloji Ana Bilim Dalı
dc.contributor.orcid0000-0001-6261-4233
dc.contributor.researcheridAAH-1448-2021
dc.contributor.scopusid6508023759
dc.date.accessioned2023-04-14T07:22:53Z
dc.date.available2023-04-14T07:22:53Z
dc.date.issued2016-02-27
dc.description.abstractThis study tested the hypothesis that the hypothalamus participates in the decompensatory phase of hemorrhage by measuring Fos immunoreactivity and by inhibiting neuronal activity in selected hypothalamic nuclei with lidocaine or cobalt chloride. Previously, we reported that inactivation of the arcuate nucleus inhibited, but did not fully prevent, the fall in arterial pressure evoked by hypotensive hemorrhage. Here, we report that hemorrhage (2.2 ml/100 g body weight over 20 min) induced Fos expression in a high percentage of cells in the paraventricular, supraoptic and arcuate nuclei of the hypothalamus as shown previously. Lower densities of Fos immunoreactive cells were also found in the medial preoptic area (mPOA), anterior hypothalamus, lateral hypothalamus (LH), dorsomedial hypothalamus, ventromedial hypothalamus (VMH) and posterior hypothalamus. Bilateral injection of lidocaine (2%; 0.1 mu l or 0.3 mu l) or cobalt chloride (5 mM; 0.3 mu l) into the tuberal portion of the LH immediately before hemorrhage was initiated reduced the magnitude of hemorrhagic hypotension and bradycardia significantly. Lidocaine injection into the VMH also attenuated the fall in arterial pressure and heart rate evoked by hemorrhage although inactivation of the mPOA or rostral LH was ineffective. These findings indicate that hemorrhage activates neurons throughout much of the hypothalamus and that a relatively broad area of the hypothalamus, extending from the arcuate nucleus laterally through the caudal VMH and tuberal LH, plays an important role in the decompensatory phase of hemorrhage.
dc.description.sponsorshipOffice of Naval Research - N00014-00-1-0056
dc.identifier.citationGöktalay, G. ve Millington, W. R. (2016). "Hypovolemic hemorrhage induces Fos expression in the rat hypothalamus: Evidence for involvement of the lateral hypothalamus in the decompensatory phase of hemorrhage". Neuroscience, 322, 464-478.
dc.identifier.endpage478
dc.identifier.issn0306-4522
dc.identifier.issn1873-7544
dc.identifier.pubmed26947128
dc.identifier.scopus2-s2.0-84960153439
dc.identifier.startpage464
dc.identifier.urihttps://doi.org/10.1016/j.neuroscience.2016.02.068
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0306452216002177
dc.identifier.urihttp://hdl.handle.net/11452/32390
dc.identifier.volume322
dc.identifier.wos000372349800040
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherElsevier
dc.relation.collaborationYurt dışı
dc.relation.journalNeuroscience
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectNeurosciences & neurology
dc.subjectHemorrhagic shock
dc.subjectLateral hypothalamus
dc.subjectVentromedial hypothalamus
dc.subjectArcuate nucleus
dc.subjectFos immunoreactivity
dc.subjectVentrolateral periaqueductal gray
dc.subjectNucleus-tractus-solitarius
dc.subjectDelta-opioid receptors
dc.subjectVulgaris-leukoagglutinin
dc.subjectCardiovascular-responses
dc.subjectAnterior hypothalamus
dc.subjectEfferent connections
dc.subjectL-glutamate
dc.subjectBrain-stem
dc.subjectExcitatory projections
dc.subjectHemorrhage
dc.subject.emtreeCobalt chloride
dc.subject.emtreeLidocaine
dc.subject.emtreeProtein fos
dc.subject.emtreeCobalt
dc.subject.emtreeHemostatic agent
dc.subject.emtreeLidocaine
dc.subject.emtreeProtein c fos
dc.subject.emtreeAnimal cell
dc.subject.emtreeAnimal experiment
dc.subject.emtreeAnimal model
dc.subject.emtreeAnimal tissue
dc.subject.emtreeAnterior hypothalamus
dc.subject.emtreeArcuate nucleus
dc.subject.emtreeArterial pressure
dc.subject.emtreeArticle
dc.subject.emtreeBleeding
dc.subject.emtreeBody weight
dc.subject.emtreeBradycardia
dc.subject.emtreeBrain function
dc.subject.emtreeControlled study
dc.subject.emtreeDisease duration
dc.subject.emtreeDorso medial hypothalamus
dc.subject.emtreeEvoked response
dc.subject.emtreeHeart rate
dc.subject.emtreeHypotension
dc.subject.emtreeHypothalamic paraventricular nucleus
dc.subject.emtreeHypothalamus
dc.subject.emtreeHypovolemia
dc.subject.emtreeHypovolemic hemorrhage
dc.subject.emtreeImmunoreactivity
dc.subject.emtreeLateral hypothalamus
dc.subject.emtreeMale
dc.subject.emtreeMedial preoptic area
dc.subject.emtreeNonhuman
dc.subject.emtreePathogenesis
dc.subject.emtreePosterior hypothalamus
dc.subject.emtreePriority journal
dc.subject.emtreeProtein expression
dc.subject.emtreeRat
dc.subject.emtreeSynapse
dc.subject.emtreeZona incerta
dc.subject.emtreeAnimal
dc.subject.emtreeBleeding
dc.subject.emtreeDisease model
dc.subject.emtreeHypovolemia
dc.subject.emtreeMetabolism
dc.subject.emtreeNerve cell
dc.subject.emtreePathology
dc.subject.emtreePathophysiology
dc.subject.emtreeSprague dawley rat
dc.subject.meshAnimals
dc.subject.meshBradycardia
dc.subject.meshCobalt
dc.subject.meshDisease models, animal
dc.subject.meshHemorrhage
dc.subject.meshHemostatics
dc.subject.meshHypothalamus
dc.subject.meshHypovolemia
dc.subject.meshLidocaine
dc.subject.meshMale
dc.subject.meshNeurons
dc.subject.meshProto-oncogene proteins c-fos
dc.subject.meshRats, sprague-dawley
dc.subject.scopusPeriaqueductal Gray; Animals; Escape Reaction
dc.subject.wosNeurosciences
dc.titleHypovolemic hemorrhage induces Fos expression in the rat hypothalamus: Evidence for involvement of the lateral hypothalamus in the decompensatory phase of hemorrhage
dc.typeArticle
dc.wos.quartileQ2
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Tıbbi Farmakoloji Ana Bilim Dalı
local.indexed.atScopus
local.indexed.atWOS

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