Positive effect of autologous platelet rich plasma on saanen buck semen cryopreservation in non-breeding season

Abstract

The objective of the current study was to evaluate the effect of autologous platelet-rich plasma (PRP) addition into soybean lecithin based extender on buck semen at post-thaw. Semen samples were collected from eight Saanen buck, and each semen sample was split into four equal aliquots and diluted with different concentrations of PRP supplemented extenders [no PRP (control), 0.5 x 107/ml PRP, 1 x 107/ml PRP, or 2 x 107/ml PRP]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed at post-thaw. The results showed that 2 x 107/ml PRP group had a positive effect on motility (62.41 +/- 4.24), membrane functional integrity (71.11 +/- 2.90), mitochondrial membrane potential (69.70 +/- 1.99), DNA integrity (7.22 +/- 0.93) and MDA levels (2.56 +/- 0.73) at post-thaw (P < 0.05). The results of the study demonstrated that autologous PRP has a protective effect on cryopreservation of buck spermatozoa and the fertility effects are worthy of further study.