Publication:
Glutamatergic activation of neuronostatin neurons in the periventricular nucleus of the hypothalamus

dc.contributor.authorKoçoğlu, Sema Serter
dc.contributor.authorYurtseven, Duygu Gök
dc.contributor.buuauthorÇakır, Cihan
dc.contributor.buuauthorMinbay, Zehra
dc.contributor.buuauthorEyigör, Özhan
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentHistoloji ve Embriyoloji Ana Bilim Dalı
dc.contributor.orcid0000-0003-3463-7483
dc.contributor.researcheridERI-3853-2022
dc.contributor.researcheridABC-1475-2020
dc.contributor.researcheridABE-5128-2020
dc.contributor.scopusid57205145865
dc.contributor.scopusid8220935200
dc.contributor.scopusid6603109907
dc.date.accessioned2023-10-20T08:39:13Z
dc.date.available2023-10-20T08:39:13Z
dc.date.issued2020-04
dc.description.abstractNeuronostatin, a newly identified anorexigenic peptide, is present in the central nervous system. We tested the hypothesis that neuronostatin neurons are activated by feeding as a peripheral factor and that the glutamatergic system has regulatory influences on neuronostatin neurons. The first set of experiments analyzed the activation of neuronostatin neurons by refeeding as a physiological stimulus and the effectiveness of the glutamatergic system on this physiological stimulation. The subjects were randomly divided into three groups: the fasting group, refeeding group, and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX)+refeeding group. We found that refeeding increased the phosphorylated signal transducers and transcription activator-5 (pSTAT5) expression in neuronostatin-positive neurons and that the CNQX injection significantly suppressed the number of pSTAT5-expressing neuronostatin neurons. The second set of experiments analyzed the activation pathways of neuronostatin neurons and the regulating effects of the glutamatergic system on neuronostatin neurons. The animals received intraperitoneal injections of glutamate receptor agonists (kainic acid, alpha-amino-3-hydroxy-5methyl-4-isoazepropionic acid (AMPA), and N-methyl-D-aspartate (NMDA)) or 0.9% NaCl. The number of c-Fos-expressing neuronostatin neurons significantly increased following the AMPA and NMDA injections. In conclusion, we found that the neuronostatin neurons were activated by peripheral or central signals, including food intake and/or glutamatergic innervation, and that the glutamate receptors played an important role in this activation.
dc.identifier.citationKoçoğlu, S. S. vd. (2020). "Glutamatergic activation of neuronostatin neurons in the periventricular nucleus of the hypothalamus". Brain Sciences, 10(4).
dc.identifier.issn2076-3425
dc.identifier.issue4
dc.identifier.pubmed32268550
dc.identifier.scopus2-s2.0-85083962630
dc.identifier.urihttps://doi.org/10.3390/brainsci10040217
dc.identifier.urihttps://www.mdpi.com/2076-3425/10/4/217
dc.identifier.urihttp://hdl.handle.net/11452/34495
dc.identifier.volume10
dc.identifier.wos000534271500002
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherMDPI
dc.relation.collaborationYurt içi
dc.relation.journalBrain Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectNeurosciences & neurology
dc.subjectNeuronostatin
dc.subjectGlutamate
dc.subjectC-fos
dc.subjectKainic acid
dc.subjectRat-brain
dc.subjectSomatostatin
dc.subjectExpression
dc.subjectLocalization
dc.subjectTransporters
dc.subjectPathway
dc.subject.emtree6 cyano 7 nitro 2,3 quinoxalinedione
dc.subject.emtreeLpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid
dc.subject.emtreeGlutamate receptor
dc.subject.emtreeGlutamate receptor antagonist
dc.subject.emtreeGlutamic acid
dc.subject.emtreeKainic acid
dc.subject.emtreeN methyl dextro aspartic acid
dc.subject.emtreeNeuronostatin
dc.subject.emtreeNeuropeptide
dc.subject.emtreeNeurotransmitter
dc.subject.emtreeProtein c fos
dc.subject.emtreeStat5 protein
dc.subject.emtreeUnclassified drug
dc.subject.emtreeAnimal experiment
dc.subject.emtreeAnimal model
dc.subject.emtreeAnimal tissue
dc.subject.emtreeArcuate nucleus
dc.subject.emtreeArticle
dc.subject.emtreeCentral nervous system
dc.subject.emtreeControlled study
dc.subject.emtreeFood intake
dc.subject.emtreeHypothalamus periventricular nucleus
dc.subject.emtreeHypothalamus ventromedial nucleus
dc.subject.emtreeImmunohistochemistry
dc.subject.emtreeImmunoreactivity
dc.subject.emtreeMale
dc.subject.emtreeMedian eminence
dc.subject.emtreeNerve cell
dc.subject.emtreeNerve ending
dc.subject.emtreeNerve stimulation
dc.subject.emtreeNeuronostatin neuron
dc.subject.emtreeNonhuman
dc.subject.emtreeProtein expression
dc.subject.emtreeRat
dc.subject.emtreeStatistical analysis
dc.subject.emtreeSuprachiasmatic nucleus
dc.subject.scopusOctreotide; Somatostatin; Amino Acids
dc.subject.wosNeurosciences
dc.titleGlutamatergic activation of neuronostatin neurons in the periventricular nucleus of the hypothalamus
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Histoloji ve Embriyoloji Ana Bilim Dalı
local.indexed.atPubMed
local.indexed.atWOS

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Koçoğlu_vd_2020.pdf
Size:
4.59 MB
Format:
Adobe Portable Document Format
Description:

License bundle

Now showing 1 - 1 of 1
Placeholder
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: