Investigation of cyclin D1 (G870A) gene polymorphisms in patients with gastric carcinoma

Abstract

Objectives: Gastric carcinoma is the second most common cancer in both man and woman in Turkey. Many environmental and individual etiological factors have been investigated including genetic factors. Cyclins which have an important role in cell cycle have been studied, particularly cyclin D1 gene polymorphism has been found to have a role in some cancers. In this study, association between cyclin D1 gene polymorphism and gastric carcinoma was searched in our community.Materials and Methods: Fifty-eight gastric carcinoma patients who had been admitted at Bursa Yuksek Ihtisas Hospital General Surgery Department between 2005 and 2010 and 59 healthy individuals have been included in the study. Samples have been subjected to genetic analysis by PCR-RFLP method in Medical Genetics Department laboratory at Uludag University.Results: GG genotype was found in 16 (28%), AG genotype in 28 (49%), AA genotype in 13 (% 23) in patient group. In control group, numbers was 11 (19%), 31 (54%) and 17 (29%) respectively. The difference of GG, AG and AA genotypes between patient and control groups was not statistically significant. G allele was found 60 (53%) and an allele 54 (47%) in patient group and 51 (45%), and 66 (55%) in control group.Conclusion: Our knowledge, this study is the first to evaluate the relation between gastric carcinoma and cyclin D1 polymorphism in Turkish population. Our results show that there is no any association between gastric carcinoma and cyclin D1 polymorphism in the community which is represented by our study and control groups.