Publication:
Enhancement of enzyme activity by laser-induced energy propulsion of upconverting nanoparticles under near-infrared light: A comprehensive methodology for in vitro and in vivo applications

dc.contributor.authorAteş, Burhan
dc.contributor.authorUlu, Ahmet
dc.contributor.authorAsiltürk, Meltem
dc.contributor.authorTopel, Seda Demirel
dc.contributor.authorDik, Gamze
dc.contributor.authorÖzhan, Onural
dc.contributor.authorBakar, Buesra
dc.contributor.authorYıldız, Azibe
dc.contributor.authorVardi, Nigar
dc.contributor.authorParlakpınar, Hakan
dc.contributor.buuauthorNOMA, Samir Abbas Ali
dc.contributor.departmentKimya Ana Bilim Dalı.
dc.contributor.departmentFen Edebiyat Fakültesi
dc.contributor.orcid0000-0001-6080-229X
dc.contributor.orcid0000-0002-4618-5712
dc.contributor.orcid0000-0001-9497-3468
dc.contributor.researcheridAAA-3730-2021
dc.contributor.researcheridABH-1773-2021
dc.contributor.researcheridC-9549-2018
dc.contributor.researcheridT-6517-2018
dc.contributor.researcheridABI-7998-2020
dc.contributor.researcheridV-6637-2019
dc.date.accessioned2025-02-18T06:02:33Z
dc.date.available2025-02-18T06:02:33Z
dc.date.issued2024-01-23
dc.description.abstractIf the appropriate immobilization method and carrier support are not selected, partial decreases in the activity of enzymes may occur after immobilization. Herein, to overcome this challenge, an excitation mechanism that enables energy transfer was proposed. Modified upconverting nanoparticles (UCNPs) were constructed and the important role of near-infrared (NIR) excitation in enhancing the catalytic activity of the enzyme was demonstrated. For this purpose, UCNPs were first synthesized via the hydrothermal method, functionalized with isocyanate groups, and then, PEG-L-ASNase was immobilized via covalent binding. UCNPs with and without PEG-LASNase were extensively characterized by different methods. These supports had immobilization yield and activity efficiency of >96 % and 78 %, respectively. Moreover, immobilized enzymes exhibited improved pH, thermal, and storage stability. In addition, they retained >65 % of their initial activity even after 20 catalytic cycles. Biochemical and histological findings did not indicate a trend of toxicity in rats due to UCNPs. Most importantly, PEG-L-ASNase activity was triggered approximately 5- and 2-fold under in vitro and in vivo conditions, respectively. Overall, it is anticipated that this pioneering work will shed new light on the realistic and promising usage of NIR-excited UCNPs for the immobilization of enzymes in expensive and extensive applications.
dc.description.sponsorshipThe "2515-COST (European Cooperation in Science and Technology" of the Scientific and Technological Research Council of Turkiye 119Z962 CA17126
dc.identifier.doi10.1016/j.ijbiomac.2024.129343
dc.identifier.issn0141-8130
dc.identifier.scopus2-s2.0-85183315524
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2024.129343
dc.identifier.urihttps://hdl.handle.net/11452/50495
dc.identifier.volume260
dc.identifier.wos001174291500001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherElsevier
dc.relation.journalInternational Journal Of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectUp-conversion nanoparticles
dc.subjectImmobilization strategies
dc.subjectCovalent immobilization
dc.subjectGraphene oxide
dc.subjectL-asparaginase
dc.subjectBiodistribution
dc.subjectNanomaterials
dc.subjectNanocarriers
dc.subjectNanoplatform
dc.subjectStability
dc.subjectEnzyme immobilization
dc.subjectPeg-l-asparaginase
dc.subjectUpconverting nanoparticles (ucnps)
dc.subjectNear-infrared (nir) excitation
dc.subjectTriggered activity
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectBiochemistry & molecular biology
dc.subjectChemistry, applied
dc.subjectPolymer science
dc.subjectChemistry
dc.titleEnhancement of enzyme activity by laser-induced energy propulsion of upconverting nanoparticles under near-infrared light: A comprehensive methodology for in vitro and in vivo applications
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentFen Edebiyat Fakültesi/Kimya Ana Bilim Dalı.
local.indexed.atWOS
local.indexed.atScopus
relation.isAuthorOfPublication08ae8d1b-5dad-4ab3-8186-7723e086d163
relation.isAuthorOfPublication.latestForDiscovery08ae8d1b-5dad-4ab3-8186-7723e086d163

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