Publication:
The effects of hypoxia on the expression of vascular endothelial growth factor in broiler lung fibroblasts

dc.contributor.authorÖzyiğit, Musa Özgür
dc.contributor.authorKahraman, Müjdat Müfit
dc.contributor.authorAkkoç, Ahmet
dc.contributor.buuauthorÖZYİĞİT, MUSA ÖZGÜR
dc.contributor.buuauthorKAHRAMAN, MÜJDAT MÜFİT
dc.contributor.buuauthorAKKOÇ, AHMET
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.
dc.contributor.orcid0000-0002-5090-7917
dc.contributor.researcheridAAH-2873-2021
dc.contributor.researcheridCYB-9080-2022
dc.contributor.researcheridDTZ-3578-2022
dc.date.accessioned2024-10-25T12:56:25Z
dc.date.available2024-10-25T12:56:25Z
dc.date.issued2015-01-01
dc.descriptionBu çalışma, Aydın’da düzenlenen 6. Veterinary Pathology Congress Kongresi‘nde bildiri olarak sunulmuştur.
dc.descriptionBu çalışma, İstanbul’da düzenlenen 36. European Congress of Cytology Kongresi‘nde bildiri olarak sunulmuştur.
dc.description.abstractThe aim of this study was to investigate the effects of hypoxic stresses on vascular endothelial growth factor (VEGF) in cultured lung fibroblasts of meat-type chickens. Isolated and subcultured primary lung fibroblasts were exposed to hypoxia. The presence of VEGF and the effects of hypoxia on cultured lung fibroblasts and in the culture media were evaluated. Immunocytochemistry using streptavidin-biotin-peroxidase method and ELISA using commercially available primary monoclonal antibodies were used for evaluation in both 6-h and 12-h hypoxic fibroblasts. The level of VEGF was slightly higher in the 6-h hypoxic group (1.22 ng/mL) than in the 6-h control group (1.27 ng/mL) in cell culture media by ELISA. In the 6-h hypoxic group, a correlation between the decrease in immunocytochemical score and increase in medium with VEGF was observed. The presence of VEGF was shown in cultured chicken lung fibroblasts from meat-type chickens. In conclusion, a significant decrease in immunocytochemical staining score was observed along with a slight increase in the amount of VEGF in culture media under hypoxic conditions. The results suggest that VEGF might have a role in edema formation via permeability increase in the lungs of ascitic meat-type chickens.
dc.identifier.doi10.3906/vet-1410-15
dc.identifier.endpage180
dc.identifier.issn1300-0128
dc.identifier.issue2
dc.identifier.startpage174
dc.identifier.urihttps://doi.org/10.3906/vet-1410-15
dc.identifier.urihttps://journals.tubitak.gov.tr/veterinary/vol39/iss2/9/
dc.identifier.urihttps://hdl.handle.net/11452/47128
dc.identifier.volume39
dc.identifier.wos000353338100009
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherTÜBİTAK
dc.relation.journalTurkish Journal of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitak108O458
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMatrix metalloproteinases
dc.subjectPulmonary-edema
dc.subjectAscites syndrome
dc.subjectFactor vegf
dc.subjectAltitude
dc.subjectCells
dc.subjectAngiogenesis
dc.subjectPathogenesis
dc.subjectRelease
dc.subjectVascular endothelial growth factor
dc.subjectAscites
dc.subjectMeat-type chicken
dc.subjectHypoxia
dc.subjectVeterinary sciences
dc.titleThe effects of hypoxia on the expression of vascular endothelial growth factor in broiler lung fibroblasts
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication3a2cbbbb-163d-428a-9caa-27708d631560
relation.isAuthorOfPublication4513aa1c-d5ed-4d2e-bbb6-cfbc77f399a6
relation.isAuthorOfPublication77d6f8ff-e2a2-45b4-a991-48f59fee43a4
relation.isAuthorOfPublication.latestForDiscovery3a2cbbbb-163d-428a-9caa-27708d631560

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