Publication: In vitro antigenotoxicity of ulva rigida c. agardh (chlorophyceae) extract against induction of chromosome aberration, sister chromatid exchange and micronuclei by mutagenic agent MMC
Date
Authors
Çelikler, Serap
Yıldız, Gamze
Vatan, Özgür
Bilaloǧlu, Rahmi
Authors
Advisor
Language
Type
Publisher:
Chinese Center Disaese Control & Prevention
Journal Title
Journal ISSN
Volume Title
Abstract
Objective To determine the in vitro possible clastogenic and cytotoxic activities of Ova rigida crude extracts (URE), and identify their antigenotoxic and protective effects on chemotherapeutic agent mitomycine-C (MMC). Methods Anti-clastogenic and anti-genotoxic activities of Ulva rigida crude extracts (URE) were studied using chromosome aberration (CA), sister chromatid exchange (SCE), and micronuclei (MN) tests in human lymphocytes cultured in vitro. Results The chromosome aberration, sister chromatid exchange or micronuclei tests showed that URE at concentrations of 10, 20, and 40 mu g/mL had no clastogenic activity in human lymphocyte cell Culture. Three doses of URE significantly decreased the number of chromosomal aberrations and the frequencies of SCE and MN when compared with the culture treated with MMC (P<0.0001). Conclusion Although URE itself is not a clastogenic or cytotoxic substance, it possesses strong antigenotoxic, anti-clastogenic, and protective effects on MMC in vitro.
Description
Source:
Keywords:
Keywords
Chlorophyceae, Ulva, Ulva rigida, Chromosomal aberration, Antigenotoxicity, Anticlastogenicity, Micronuclei, Mitomycin-C, Sister chromatid exchange, Ulva rigida, Wakame undaria-pinnatifida, Antibacterial activity, Marine-algae, Natural-products, Seaweed, Cells, Purification, Antioxidants, Carotenoids, Carcinoma, Environmental sciences & ecology, Public, environmental & occupational health
Citation
Çelikler, S. vd. (2008). ''In vitro antigenotoxicity of ulva rigida c. agardh (chlorophyceae) extract against induction of chromosome aberration, sister chromatid exchange and micronuclei by mutagenic agent MMC''. Biomedical and Environmental Sciences, 21(6), 492-498.