Publication: Investigation of cerbb2 at mrna level in patients with gastric adenocarcinoma
dc.contributor.author | Turhan, Ezgi Işıl | |
dc.contributor.buuauthor | Aksoy, Seçil Ak | |
dc.contributor.buuauthor | UĞRAŞ, NESRİN | |
dc.contributor.buuauthor | IŞIK, ÖZGEN | |
dc.contributor.buuauthor | AKSOY, SEÇİL | |
dc.contributor.buuauthor | TUNCA, BERRİN | |
dc.contributor.buuauthor | Tunca, Berrin | |
dc.contributor.buuauthor | Işık, Özgen | |
dc.contributor.buuauthor | Yerci, Ömer | |
dc.contributor.buuauthor | YERCİ, ÖMER | |
dc.contributor.buuauthor | Vuruşkan, Berna Aytaç | |
dc.contributor.buuauthor | AYTAÇ VURUŞKAN, BERNA | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Klinik Biyoloji Anabilim Dalı. | |
dc.contributor.orcid | 0000-0002-1619-6680 | |
dc.contributor.orcid | 0000-0002-9541-5035 | |
dc.contributor.researcherid | ADM-8457-2022 | |
dc.contributor.researcherid | AAW-9602-2020 | |
dc.date.accessioned | 2024-09-17T05:28:20Z | |
dc.date.available | 2024-09-17T05:28:20Z | |
dc.date.issued | 2022-06-01 | |
dc.description.abstract | Aim: Gastric adenocarcinomas take place near the top regarding mortality due to cancer. This study aims to validate IHC results with the RT-PCR method and to evaluate their contribution to confirm the absolute score of CerbB2 situation in tissues. Material and Methods: We analyzed 80 gastric adenocarcinoma cases diagnosed in our clinic. The expression characteristics of the cases were evaluated using CerbB2 staining. Simultaneously, CerbB2 expression analyses were performed with the RT-PCR method. Results: Positive immunoreactivity was observed in 19 of 80 cases (23,75%) in the study conducted using CerbB2 antibody. While 14 of these cases (17.5%) demonstrated weak positive (2+), 5 of them (6.25%) demonstrated strong positive (3+) immunoreactivity. With the RT-PCR method, an increase in gene expression was observed in 12 of 14 weak positive cases (75%). In all 5 strong positive cases, on the other hand, high gene expression was determined. Between CerbB2 immunohistochemical findings and gene expression, 89% compatibility and a statistically significant relationship was determined. While an increase in CerbB2 gene expression, determined by Real Tirme PCR method, was not observed in 57 of 61 cases (96.6%) without CerbB2 immunoreactivity, 4 cases had an increase in CerbB2 expression in comparison to normal. In our study, a statistically significant relationship was determined between CerbB2 expression and Helicobacter pylori (H. pylori) that is a factor blamed for gastric cancer etiology. The relationship between CerbB2 expression and clinicopathologic prognostic factors has been statistically reviewed; no significant results have been found. Discussion: Our study has shown that RT-PCR is a method, which might be an alternative to IHC and in ISH methods. It is concluded that a statistically significant relationship might be determined between CerbB2 expression and clinicopathologic prognostic parameters by increasing the case number. | |
dc.identifier.doi | 10.4328/ACAM.21065 | |
dc.identifier.endpage | 644 | |
dc.identifier.issue | 6 | |
dc.identifier.startpage | 640 | |
dc.identifier.uri | https://doi.org/10.4328/ACAM.21065 | |
dc.identifier.uri | https://hdl.handle.net/11452/44803 | |
dc.identifier.volume | 13 | |
dc.identifier.wos | 001027513600011 | |
dc.indexed.wos | WOS.ESCI | |
dc.language.iso | en | |
dc.publisher | Bayrakol Medical Publisher | |
dc.relation.journal | Annals Of Clinical And Analytical Medicine | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Gene amplification | |
dc.subject | Rt-pcr | |
dc.subject | Cancer | |
dc.subject | Overexpression | |
dc.subject | Expression | |
dc.subject | Gastric adenocarcinoma | |
dc.subject | Cerbb2 | |
dc.subject | Immunohistochemistry | |
dc.subject | Clinicopathologic | |
dc.subject | Pcr | |
dc.subject | Science & technology | |
dc.subject | Life sciences & biomedicine | |
dc.subject | Medicine, general & internal | |
dc.subject | General & internal medicine | |
dc.title | Investigation of cerbb2 at mrna level in patients with gastric adenocarcinoma | |
dc.type | Article | |
dspace.entity.type | Publication | |
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