Publication:
Investigation of cerbb2 at mrna level in patients with gastric adenocarcinoma

dc.contributor.authorTurhan, Ezgi Işıl
dc.contributor.buuauthorAksoy, Seçil Ak
dc.contributor.buuauthorUĞRAŞ, NESRİN
dc.contributor.buuauthorIŞIK, ÖZGEN
dc.contributor.buuauthorAKSOY, SEÇİL
dc.contributor.buuauthorTUNCA, BERRİN
dc.contributor.buuauthorTunca, Berrin
dc.contributor.buuauthorIşık, Özgen
dc.contributor.buuauthorYerci, Ömer
dc.contributor.buuauthorYERCİ, ÖMER
dc.contributor.buuauthorVuruşkan, Berna Aytaç
dc.contributor.buuauthorAYTAÇ VURUŞKAN, BERNA
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Klinik Biyoloji Anabilim Dalı.
dc.contributor.orcid0000-0002-1619-6680
dc.contributor.orcid0000-0002-9541-5035
dc.contributor.researcheridADM-8457-2022
dc.contributor.researcheridAAW-9602-2020
dc.date.accessioned2024-09-17T05:28:20Z
dc.date.available2024-09-17T05:28:20Z
dc.date.issued2022-06-01
dc.description.abstractAim: Gastric adenocarcinomas take place near the top regarding mortality due to cancer. This study aims to validate IHC results with the RT-PCR method and to evaluate their contribution to confirm the absolute score of CerbB2 situation in tissues. Material and Methods: We analyzed 80 gastric adenocarcinoma cases diagnosed in our clinic. The expression characteristics of the cases were evaluated using CerbB2 staining. Simultaneously, CerbB2 expression analyses were performed with the RT-PCR method. Results: Positive immunoreactivity was observed in 19 of 80 cases (23,75%) in the study conducted using CerbB2 antibody. While 14 of these cases (17.5%) demonstrated weak positive (2+), 5 of them (6.25%) demonstrated strong positive (3+) immunoreactivity. With the RT-PCR method, an increase in gene expression was observed in 12 of 14 weak positive cases (75%). In all 5 strong positive cases, on the other hand, high gene expression was determined. Between CerbB2 immunohistochemical findings and gene expression, 89% compatibility and a statistically significant relationship was determined. While an increase in CerbB2 gene expression, determined by Real Tirme PCR method, was not observed in 57 of 61 cases (96.6%) without CerbB2 immunoreactivity, 4 cases had an increase in CerbB2 expression in comparison to normal. In our study, a statistically significant relationship was determined between CerbB2 expression and Helicobacter pylori (H. pylori) that is a factor blamed for gastric cancer etiology. The relationship between CerbB2 expression and clinicopathologic prognostic factors has been statistically reviewed; no significant results have been found. Discussion: Our study has shown that RT-PCR is a method, which might be an alternative to IHC and in ISH methods. It is concluded that a statistically significant relationship might be determined between CerbB2 expression and clinicopathologic prognostic parameters by increasing the case number.
dc.identifier.doi10.4328/ACAM.21065
dc.identifier.endpage644
dc.identifier.issue6
dc.identifier.startpage640
dc.identifier.urihttps://doi.org/10.4328/ACAM.21065
dc.identifier.urihttps://hdl.handle.net/11452/44803
dc.identifier.volume13
dc.identifier.wos001027513600011
dc.indexed.wosWOS.ESCI
dc.language.isoen
dc.publisherBayrakol Medical Publisher
dc.relation.journalAnnals Of Clinical And Analytical Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGene amplification
dc.subjectRt-pcr
dc.subjectCancer
dc.subjectOverexpression
dc.subjectExpression
dc.subjectGastric adenocarcinoma
dc.subjectCerbb2
dc.subjectImmunohistochemistry
dc.subjectClinicopathologic
dc.subjectPcr
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectMedicine, general & internal
dc.subjectGeneral & internal medicine
dc.titleInvestigation of cerbb2 at mrna level in patients with gastric adenocarcinoma
dc.typeArticle
dspace.entity.typePublication
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relation.isAuthorOfPublication121a3732-be5d-4aff-9195-357c8347daca
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relation.isAuthorOfPublication.latestForDiscovery8bdd2606-375e-4245-8bdc-c609f605bfd4

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