Publication:
Vascular functional effect mechanisms of elabela in rat thoracic aorta

dc.contributor.buuauthorŞahintürk, Serdar
dc.contributor.buuauthorŞAHİNTÜRK, SERDAR
dc.contributor.buuauthorÖZYENER, FADIL
dc.contributor.buuauthorDemirel, Sadettin
dc.contributor.buuauthorİŞBİL, NACİYE
dc.contributor.buuauthorDEMİREL, SADETTİN
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentFizyoloji Ana Bilim Dalı
dc.contributor.researcheridAAH-3460-2021
dc.contributor.researcheridAAH-1641-2021
dc.contributor.researcheridACQ-9887-2022
dc.date.accessioned2024-11-06T11:29:11Z
dc.date.available2024-11-06T11:29:11Z
dc.date.issued2022-08-01
dc.description.abstractBackground: Elabela is a recently discovered peptide hormone. The present study aims to investigate the vasorelaxant effect mechanisms of elabela in the rat thoracic aorta.Methods: The vascular rings obtained from the thoracic aortas of the male Wistar albino rats were placed in the isolated tissue bath system. Resting tension was set to 1 gram. After the equilibration period, the vessel rings were contracted with phenylephrine or potassium chloride. Once a stable contraction was achieved, elabela-32 was applied cumulatively (10(-9)-10(-6) molar) to the vascular rings. The experimental protocol was repeated in the presence of specific signaling pathway inhibitors or potassium channel blockers to determine the effect mechanisms of elabela.Results: Elabela showed a significant vasorelaxant effect in a concentration-dependent manner (P < 0.001). The vasorelaxant effect level of elabela was significantly reduced by the apelin receptor antagonist F13A, cyclooxygenase inhibitor indomethacin, adenosine monophosphate-activated protein kinase inhibitor dorsomorphin, protein kinase C inhibitor bisindolmaleimide, large-conductance calcium-activated potassium channel blocker iberiotoxin, and intermediate-conductance calcium-activated potassium channel blocker TRAM-34 (P < 0.001). However, the vasorelaxant effect level of elabela was not significantly affected by the endothelial nitric oxide synthase inhibitor nitro-L-arginine methyl ester and mitogen-activated protein kinase inhibitor U0126.Conclusions: Elabela exhibits a prominent vasodilator effect in rat thoracic aorta. Apelin receptor, prostanoids, adenosine monophosphate-activated protein kinase, protein kinase C, and calcium-activated potassium channels are involved in the vasorelaxant effect mechanisms of elabela.
dc.identifier.doi10.1016/j.avsg.2022.04.033
dc.identifier.endpage397
dc.identifier.issn0890-5096
dc.identifier.startpage381
dc.identifier.urihttps://doi.org/10.1016/j.avsg.2022.04.033
dc.identifier.urihttps://hdl.handle.net/11452/47486
dc.identifier.volume84
dc.identifier.wos000855847700041
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.journalAnnals Of Vascular Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitak119S971
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectActivated protein-kinase
dc.subjectResistance arteries
dc.subjectBlood-pressure
dc.subjectAngiotensin-ii
dc.subjectHeart-failure
dc.subjectApelin
dc.subjectPreeclampsia
dc.subjectChannels
dc.subjectDisease
dc.subjectVasoconstriction
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectSurgery
dc.subjectPeripheral vascular disease
dc.subjectCardiovascular system & cardiology
dc.titleVascular functional effect mechanisms of elabela in rat thoracic aorta
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Fizyoloji Ana Bilim Dalı
relation.isAuthorOfPublication25bede72-9942-49c8-b45d-1e94eaf9062d
relation.isAuthorOfPublication4c0e0603-772f-4429-b7ca-9d8e68702800
relation.isAuthorOfPublication6459c031-8ea7-4356-91ed-9d11cffa5a69
relation.isAuthorOfPublicationbf421fa5-e949-4453-b2b2-c4a9df1be392
relation.isAuthorOfPublication.latestForDiscovery25bede72-9942-49c8-b45d-1e94eaf9062d

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