Rift valley fever virus infectivity in milk stored at a wide range of temperatures

Abstract

Background Rift Valley fever virus (RVFV) is a zoonotic arbovirus widely distributed across Africa and the Arabian Peninsula, which causes devastating epidemics affecting humans as well as ruminant livestock. The epidemiology of RVFV is difficult to disentangle, as it is transmitted to humans via mosquitos or direct contact with infected animals. Among these direct exposures are milking ruminants and drinking fresh milk, which have been firmly established as risk factors for RVFV exposure. However, no studies to date have addressed the stability of infectious RVFV in milk.Methods We assessed the stability of RVFV in milk by spiking cow's and goat's milk with infectious RVFV-MP12 vaccine strain and measuring RNA via quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and infectious viral titers via plaque assay over time in a variety of temperature conditions.Results We demonstrate that RVFV remains stable for up to 96 hours in refrigerated milk and up to 2 days in milk stored in warm ambient conditions. We also show that RVFV RNA remained detectable via qRT-PCR in milk at all assessed temperatures for up to 96 hours. Finally, we confirmed that commonly performed pasteurization techniques and boiling of milk fully inactivates RVFV in milk.Conclusions These results demonstrate that RVFV contaminated milk in the milk supply chain of endemic regions could represent a widespread public health risk, and much of this risk is mitigatable via pasteurization. We also propose use of RVFV RNA detection in milk as a noninvasive source of RVFV surveillance.Rift Valley fever virus (RVFV) is a highly pathogenic zoonotic arbovirus transmitted via mosquitos and contact with animals. Milk exposure is a known risk factor. Here we show that RVFV remains stable in cow's and goat's milk for several days.