Publication:
Effect of butylated hydroxytoluene on post-thawed semen quality of beetal goat buck, capra hircus

dc.contributor.authorIqbal, Z.
dc.contributor.authorIjaz, A.
dc.contributor.authorAleem, M.
dc.contributor.authorShahzad, A. H.
dc.contributor.authorSohail, M. U.
dc.contributor.authorNak, Deniz
dc.contributor.authorNak, Yavuz
dc.contributor.authorAbbas, S.
dc.contributor.buuauthorNAK, DENİZ
dc.contributor.buuauthorNAK, YAVUZ
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentDoğum ve Jinekoloji Bölümü
dc.contributor.researcheridAAH-5494-2021
dc.contributor.researcheridFPJ-3706-2022
dc.date.accessioned2024-08-13T05:48:00Z
dc.date.available2024-08-13T05:48:00Z
dc.date.issued2015-02-01
dc.description.abstractPresent study was conducted to evaluate the effect butylated hydroxytoluene (BHT) on post-thawed semen quality of Beetal goat buck semen. Semen was obtained from six bucks using artificial vagina and cryopreserved in tris egg yolk extender and semen quality was assessed on the basis of post-thaw sperm motility, viability, plasma membrane integrity, and acrosomal membrane status. Ejaculates were pooled and extended to the concentration of 2 x 10(9) spermatozoa per mL in tris egg yolk extender (300 mOsmol/L). Tris egg yolk extender containing various concentrations of BHT (0.0, 2.0 and 5.0 mM) was prepared. French straws (0.5 mL) were manually filled with semen, gradually cooled from 39 degrees C to 4 degrees C and finally, cryopreserved in liquid nitrogen at -196 degrees C. Five straws from each treatment [BHT (0.0, 2.0 and 5.0 mM)] were thawed and evaluated under phase-contrast microscope (40 x) for sperm motility, whereas sperm viability, plasma membrane integrity and acrosomal integrity were assessed by the supravital staining, hypo-osmotic swelling test (HOST) and normal acrosomal reaction, respectively. The results showed that only acrosomal integrity was improved (P < 0.05) by the addition of BHT in semen extender. Motility was suppressed (P < 0.05) by increasing BHT concentration. The maximum motility of sperm was achieved with 0.0 mM BHT. The HOST response and viability of spermatozoa were increased by addition of 2 and 5 mM BHT, but this increment was not statistically significant. In conclusion, the addition of BHT to semen extender can partially improve semen quality of Beetal goat.
dc.identifier.endpage124
dc.identifier.issn0030-9923
dc.identifier.issue1
dc.identifier.startpage119
dc.identifier.urihttps://hdl.handle.net/11452/43945
dc.identifier.volume47
dc.identifier.wos000350843000016
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherZoological Soc Pakistan
dc.relation.journalPakistan Journal of Zoology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBull sperm
dc.subjectBoar spermatozoa
dc.subjectMale-infertility
dc.subjectFertility
dc.subjectExtender
dc.subjectCryopreservation
dc.subjectAntioxidants
dc.subjectFrozen
dc.subjectStorage
dc.subjectStress
dc.subjectSemen
dc.subjectButylated hydroxytoluene
dc.subjectGoat buck
dc.subjectCryopreservation
dc.subjectSemen quality
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectZoology
dc.titleEffect of butylated hydroxytoluene on post-thawed semen quality of beetal goat buck, capra hircus
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Doğum ve Jinekoloji Bölümü
relation.isAuthorOfPublicationafe88796-1ecb-450e-b311-5217b64113db
relation.isAuthorOfPublicationabc48dbd-d82b-49de-9209-3898b6506f16
relation.isAuthorOfPublication.latestForDiscoveryafe88796-1ecb-450e-b311-5217b64113db

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