Publication: Effect of freezing rate on goat sperm morphology and DNA integrity
dc.contributor.buuauthor | Üstüner, Burcu | |
dc.contributor.buuauthor | Nur, Zekariya | |
dc.contributor.buuauthor | Alcay, Selim | |
dc.contributor.buuauthor | Toker, Mehmet Berk | |
dc.contributor.buuauthor | Sağırkaya, Hakan | |
dc.contributor.buuauthor | Soylu, Mustafa Kemal | |
dc.contributor.department | Veteriner Fakültesi | |
dc.contributor.department | Dölerme ve Suni Tohumlama Ana Bilim Dalı | |
dc.contributor.orcid | 0000-0002-1438-221X | |
dc.contributor.orcid | 0000-0003-4033-9749 | |
dc.contributor.researcherid | AAG-7238-2021 | |
dc.contributor.researcherid | AAH-2635-2021 | |
dc.contributor.researcherid | CBC-7350-2022 | |
dc.contributor.researcherid | A-2794-2014 | |
dc.contributor.researcherid | AAH-8821-2021 | |
dc.contributor.researcherid | HKG-7604-2023 | |
dc.contributor.scopusid | 18937724600 | |
dc.contributor.scopusid | 6508060684 | |
dc.contributor.scopusid | 56099810300 | |
dc.contributor.scopusid | 56480349200 | |
dc.contributor.scopusid | 6602400461 | |
dc.contributor.scopusid | 7003293300 | |
dc.date.accessioned | 2024-02-20T11:39:36Z | |
dc.date.available | 2024-02-20T11:39:36Z | |
dc.date.issued | 2015 | |
dc.description.abstract | This study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different freezing rates from +5 degrees C to -150 degrees C (G10: 10 degrees C/min, G12: 12 degrees C/min, G15: 15 degrees C/min, and G24: 24 degrees C/min) and stored in liquid nitrogen until use. Semen samples were examined for sperm motility, defective acrosomes (FITC-PSA), and DNA integrity using TUNEL after dilution with extender A at equilibration and postthaw stage. There was no significant difference among the freezing stages in terms of DNA fragmentation (P > 0.05). DNA integrity was partially affected by the freezing rate. The increase of freezing rate from 10 degrees C/min to 24 degrees C/min between +5 degrees C and -150 degrees C resulted in higher postthaw DNA damage. The study found that the freeze-thawing process is detrimental to postthawed goat semen motility (P < 0.05), acrosome integrity (P < 0.05), and DNA integrity (P > 0.05). Although the freezing rates used in the present study had no effect on postthaw sperm motility and acrosome integrity (P > 0.05), sperm DNA integrity was affected. | |
dc.identifier.citation | Üstüner, B. vd. (2015). "Effect of freezing rate on goat sperm morphology and DNA integrity". Turkish Journal of Veterinary and Animal Sciences, 39(1), 110-114. | |
dc.identifier.endpage | 114 | |
dc.identifier.issn | 1300-0128 | |
dc.identifier.issue | 1 | |
dc.identifier.scopus | 2-s2.0-84920876763 | |
dc.identifier.startpage | 110 | |
dc.identifier.uri | https://doi.org/10.3906/vet-1407-70 | |
dc.identifier.uri | https://journals.tubitak.gov.tr/veterinary/vol39/iss1/17/ | |
dc.identifier.uri | https://hdl.handle.net/11452/39869 | |
dc.identifier.volume | 39 | |
dc.identifier.wos | 000347678600017 | |
dc.indexed.scopus | Scopus | |
dc.indexed.trdizin | TrDizin | |
dc.indexed.wos | SCIE | |
dc.language.iso | en | |
dc.publisher | TÜBİTAK | |
dc.relation.bap | UAPV2008/51 | |
dc.relation.journal | Turkish Journal of Veterinary and Animal Sciences | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Veterinary sciences | |
dc.subject | DNA integrity | |
dc.subject | Freezing rate | |
dc.subject | Goat semen | |
dc.subject | Cooling rate | |
dc.subject | Motility parameters | |
dc.subject | Boar spermatozoa | |
dc.subject | Semen | |
dc.subject | Cryopreservation | |
dc.subject | Fragmentation | |
dc.subject | Ram | |
dc.subject | Freezability | |
dc.subject | Plasma | |
dc.subject | Frozen | |
dc.subject.scopus | Extender; Semen; Semen extenders | |
dc.subject.wos | Veterinary sciences | |
dc.title | Effect of freezing rate on goat sperm morphology and DNA integrity | |
dc.type | Article | |
dc.wos.quartile | Q4 | |
dspace.entity.type | Publication | |
local.contributor.department | Veteriner Fakültesi/Dölerme ve Suni Tohumlama Ana Bilim Dalı | |
local.indexed.at | WOS | |
local.indexed.at | Scopus |