Publication:
Effect of freezing rate on goat sperm morphology and DNA integrity

dc.contributor.buuauthorÜstüner, Burcu
dc.contributor.buuauthorNur, Zekariya
dc.contributor.buuauthorAlcay, Selim
dc.contributor.buuauthorToker, Mehmet Berk
dc.contributor.buuauthorSağırkaya, Hakan
dc.contributor.buuauthorSoylu, Mustafa Kemal
dc.contributor.departmentVeteriner Fakültesi
dc.contributor.departmentDölerme ve Suni Tohumlama Ana Bilim Dalı
dc.contributor.orcid0000-0002-1438-221X
dc.contributor.orcid0000-0003-4033-9749
dc.contributor.researcheridAAG-7238-2021
dc.contributor.researcheridAAH-2635-2021
dc.contributor.researcheridCBC-7350-2022
dc.contributor.researcheridA-2794-2014
dc.contributor.researcheridAAH-8821-2021
dc.contributor.researcheridHKG-7604-2023
dc.contributor.scopusid18937724600
dc.contributor.scopusid6508060684
dc.contributor.scopusid56099810300
dc.contributor.scopusid56480349200
dc.contributor.scopusid6602400461
dc.contributor.scopusid7003293300
dc.date.accessioned2024-02-20T11:39:36Z
dc.date.available2024-02-20T11:39:36Z
dc.date.issued2015
dc.description.abstractThis study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different freezing rates from +5 degrees C to -150 degrees C (G10: 10 degrees C/min, G12: 12 degrees C/min, G15: 15 degrees C/min, and G24: 24 degrees C/min) and stored in liquid nitrogen until use. Semen samples were examined for sperm motility, defective acrosomes (FITC-PSA), and DNA integrity using TUNEL after dilution with extender A at equilibration and postthaw stage. There was no significant difference among the freezing stages in terms of DNA fragmentation (P > 0.05). DNA integrity was partially affected by the freezing rate. The increase of freezing rate from 10 degrees C/min to 24 degrees C/min between +5 degrees C and -150 degrees C resulted in higher postthaw DNA damage. The study found that the freeze-thawing process is detrimental to postthawed goat semen motility (P < 0.05), acrosome integrity (P < 0.05), and DNA integrity (P > 0.05). Although the freezing rates used in the present study had no effect on postthaw sperm motility and acrosome integrity (P > 0.05), sperm DNA integrity was affected.
dc.identifier.citationÜstüner, B. vd. (2015). "Effect of freezing rate on goat sperm morphology and DNA integrity". Turkish Journal of Veterinary and Animal Sciences, 39(1), 110-114.
dc.identifier.endpage114
dc.identifier.issn1300-0128
dc.identifier.issue1
dc.identifier.scopus2-s2.0-84920876763
dc.identifier.startpage110
dc.identifier.urihttps://doi.org/10.3906/vet-1407-70
dc.identifier.urihttps://journals.tubitak.gov.tr/veterinary/vol39/iss1/17/
dc.identifier.urihttps://hdl.handle.net/11452/39869
dc.identifier.volume39
dc.identifier.wos000347678600017
dc.indexed.scopusScopus
dc.indexed.trdizinTrDizin
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherTÜBİTAK
dc.relation.bapUAPV2008/51
dc.relation.journalTurkish Journal of Veterinary and Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectVeterinary sciences
dc.subjectDNA integrity
dc.subjectFreezing rate
dc.subjectGoat semen
dc.subjectCooling rate
dc.subjectMotility parameters
dc.subjectBoar spermatozoa
dc.subjectSemen
dc.subjectCryopreservation
dc.subjectFragmentation
dc.subjectRam
dc.subjectFreezability
dc.subjectPlasma
dc.subjectFrozen
dc.subject.scopusExtender; Semen; Semen extenders
dc.subject.wosVeterinary sciences
dc.titleEffect of freezing rate on goat sperm morphology and DNA integrity
dc.typeArticle
dc.wos.quartileQ4
dspace.entity.typePublication
local.contributor.departmentVeteriner Fakültesi/Dölerme ve Suni Tohumlama Ana Bilim Dalı
local.indexed.atWOS
local.indexed.atScopus

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