Autologous platelet rich plasma have positive effect on ram spermatozoa during cryopreservation in non-breeding season

Abstract

The aim of this study was to evaluate the effects of autologous platelet-rich plasma (PRP) supplemented egg yolk based extender on ram semen after thawing. Semen samples were collected from nine Kivircik rams and each semen was divided into four equal aliquots and mixed with different concentrations of PRP supported extenders [no PRP (control), 12.5x10(6)/mL PRP, 25x10(6)/mL PRP, or 50x10(6)/mL PRP)]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed after thawing. The results showed that 25x10(6)/mL PRP group had positive effect on motility (61.67 +/- 3.81), membrane functional integrity (71.00 +/- 2.96) and MDA levels (5.13 +/- 0.64) at post-thawed (P<0.05). It was determined that 25x10(6)/mL PRP and 50x10(6)/mL PRP groups were more effective than other groups in terms of mitochondrial membrane potential (69.50 +/- 1.93), acrosome integrity (78.04 +/- 2.65) and DNA integrity (5.33 +/- 0.92). The results of the study showed that autologous PRP has a protective effect in the cryopreservation of ram spermatozoa.