Yayın: Methods for lipase immobilization and their use for biodiesel production from vegetable oil
Tarih
Kurum Yazarları
Demir, Cevdet
Yazarlar
Yücel, Yasin
Dizge, Nadir
Keskinler, Bülent
Danışman
Dil
Türü
Yayıncı:
Taylor & Francis Inc
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Özet
In the present work, two different lipases (triacylglycerol hydrolase, EC 3.1.1.3), Lipozyme TL-100L and Novozyme 388, were immobilized onto three different low-cost supports using both adsorption and covalent method: celite 545, silica gel, and styrene-divinylbenzene copolymer. The maximum immobilization yield was obtained as 79.0% for Lipozyme TL-100L and the highest specific activity was 6.5 U/mg protein for Novozym 388. The properties of the support and immobilized derivatives were characterized by Fourier transform infrared spectroscopy. Maximum methyl esters yield was obtained as 98.3%. The lipases, which are immobilized by covalently, proved to be stable after even 10 repeated reuses.
Açıklama
Kaynak:
Anahtar Kelimeler:
Konusu
Alcoholysis, Environmental sciences & ecology, Biodiesel, Energy & fuels. Engineering, Lipase immobilization, Triacylglycerol hydrolase, Lipozyme tl-100l, Lipases, Novozyme 388, Styrene-divinylbenzene copolymers, Transesterification, Specific activity, Biodiesel, Lipozyme, Fourier transform infrared spectroscopy, Lipase immobilization, Silica gel, Biodiesel production, Styrene, Transesterification, Alcoholysis
Alıntı
Yücel, Y. vd. (2014). "Methods for lipase immobilization and their use for biodiesel production from vegetable oil". Energy Sources, Part A: Recovery, Utilization and Environmental Effects, 36(11), 1203-1211.