Publication:
High prevalence of class i and class ii integrons in uropathogenic e. coli strains (upecs) and their relationship with antibiotic resistance, phylogeny and virulence

dc.contributor.authorGümüş, Defne
dc.contributor.authorKalaycı Yüksek, Fatma
dc.contributor.authorCamadan, Firdevs Deniz
dc.contributor.authorOral, Merve
dc.contributor.authorKüçüker, Mine Ang
dc.contributor.buuauthorMacunluoğlu, Aslı Ceren
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentBiyoistatistik Ana Bilim Dalı
dc.date.accessioned2024-07-01T07:53:17Z
dc.date.available2024-07-01T07:53:17Z
dc.date.issued2022-01-01
dc.description.abstractObjective: Integrons, which are highly effective in capturing, integrating and expressing gene cassettes, play an important role in the dissemination of multiple antibiotic resistances. This study investigated the correlations of integrons in uropathogenic E. coli (UPEC) with antibiotic resistance, virulence and phylogeny and also the relationships of phylogroups with virulence and antibiotic resistance.Material and Methods: Fifty UPECs isolated from uncomplicated cystitis and uncomplicated pyelonephritis were investigated to detect the presence of class I, II and III integrons and phylogenetic grouping by the PCR method. Their statistical relationship with antibiotic resistance and virulence genes were investigated using our previous findings.Results: Among 50 UPEC strains, 37 (74%), 22 (44%) and only one (2%) strain was shown to harbor class I, class II and class III integrons, respectively. Twenty one (42%) strains were found to carry both class I and class II integrons. The majority of the strains were grouped as phylogroup B2 (38%) and phylogroup E (38%). The presence of integrons was in association with only ampicillin resistance (p=0.014). Integrons was found to be related neither to virulence genes nor phylogroups; however, the presence of PAI (p<0.001), ompT (p=0.035), and usp (p<0.001) genes was found to be significantly related to phylogroup B2. Phylogroup E was found to be statistically significantly correlated with co-trimoxazole resistance (p=0.043).Conclusion: Consistent with previous studies, our results have proven that there is a strong association between antibiotic resistance and the presence of integrons (especially class I) in UPEC strains, and it has been shown that integrons became very prevalent globally.
dc.identifier.doi10.26650/IUITFD.984487
dc.identifier.endpage85
dc.identifier.issue1
dc.identifier.startpage77
dc.identifier.urihttps://doi.org/10.26650/IUITFD.984487
dc.identifier.urihttps://hdl.handle.net/11452/42633
dc.identifier.volume85
dc.identifier.wos000748481200011
dc.indexed.wosWOS.ESCI
dc.language.isoen
dc.publisherİstanbul Üniversitesi
dc.relation.journalJournal Of Istanbul Faculty Of Medicine-istanbul Tip Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitak2209-a/2019
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectUrinary-tract-infections
dc.subjectEscherichia-coli
dc.subjectGene cassettes
dc.subjectAntimicrobial resistance
dc.subjectUropathogenic e. coli
dc.subjectPhylogroups
dc.subjectIntegrons
dc.subjectVirulence factors
dc.subjectAntibiotic resistance
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectMedicine, general & internal
dc.subjectGeneral & internal medicine
dc.titleHigh prevalence of class i and class ii integrons in uropathogenic e. coli strains (upecs) and their relationship with antibiotic resistance, phylogeny and virulence
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Biyoistatistik Ana Bilim Dalı

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