Publication:
Mouse model recapitulates the phenotypic heterogeneity of human adult T-cell leukemia/lymphoma in bone

dc.contributor.authorKohart, Nicole A.
dc.contributor.authorElshafae, Said M.
dc.contributor.authorSupsahvad, Wachirapan
dc.contributor.authorAlasonyalılar-Demirer, Aylin
dc.contributor.authorPanfil, Amanda R.
dc.contributor.authorXiang Jingyu
dc.contributor.authorDirksen, Wessel P.
dc.contributor.authorVeis, Deborah J.
dc.contributor.authorGreen, Patrick L.
dc.contributor.authorWeilbaecher, Katherine N.
dc.contributor.authorRosol, Thomas J.
dc.contributor.buuauthorALASONYALILAR DEMİRER, AYLİN
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı
dc.contributor.researcheridEKS-2415-2022
dc.date.accessioned2024-07-24T10:34:15Z
dc.date.available2024-07-24T10:34:15Z
dc.date.issued2019-12-01
dc.description.abstractAdult T-cell leukemia/lymphoma has a unique relationship to bone including latency in the marrow, and development of bone invasion, osteolytic tumors and humoral hypercalcemia of malignancy. To study these conditions, we established and characterized a novel mouse model of ATL bone metastasis. Patient-derived ATL cell lines including three that do not express HTLV-1 oncoprotein Tax (ATL-ED, RV-ATL, TL-0m1), an in vitro transformed human T-cell line with high Tax expression (HT-1RV), and an HTLV-1 negative T-cell lymphoma (Jurkat) were injected intratibially into NSG mice, and were capable of proliferating and modifying the bone microenvironment. Radiography, mu CT, histopathology, immunohistochemistry, plasma calcium concentrations, and qRT-PCR for several tumor-bone signaling mRNAs were performed. Luciferase-positive ATL-ED bone tumors allowed for in vivo imaging and visualization of bone tumor growth and metastasis over time. ATL-ED and HT-1RV cells caused mixed osteolytic/osteoblastic bone tumors, TL-Oml cells exhibited minimal bone involvement and aggressive local invasion into the adjacent soft tissues, Jurkat cells proliferated within bone marrow and induced minimal bone cell response, and RV-ATL cells caused marked osteolysis. This mouse model revealed important mechanisms of human ATL bone neoplasms and will be useful to investigate biological interactions, potential therapeutic targets, and new bone-targeted agents for the prevention of ATL metastases to bone.
dc.description.sponsorshipUnited States Department of Health & Human Services National Institutes of Health (NIH) - USA NIH National Cancer Institute (NCI) - P01 CA100730
dc.description.sponsorshipAppeared in source as:National Cancer Institute T32 OD010429 Appeared in source as:National Cancer Institute
dc.description.sponsorshipOhio State University Comprehensive Cancer Center
dc.description.sponsorshipUnited States Department of Health & Human Services National Institutes of Health (NIH) - USA - P30 CA016058
dc.description.sponsorshipAppeared in source as:National Institutes of Health
dc.identifier.doi10.1016/j.jbo.2019.100257
dc.identifier.issn2212-1374
dc.identifier.urihttps://doi.org/10.1016/j.jbo.2019.100257
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S2212137419300983
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911918/
dc.identifier.urihttps://hdl.handle.net/11452/43411
dc.identifier.volume19
dc.identifier.wos000506872600001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherElsevier
dc.relation.journalJournal of Bone Oncology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHormone-related protein
dc.subjectLeukemia-lymphoma
dc.subjectHumoral hypercalcemia
dc.subjectGene-expression
dc.subjectSerum-levels
dc.subjectKappa-b
dc.subjectMice
dc.subjectEngraftment
dc.subjectPatient
dc.subjectCancer
dc.subjectHtlv-1
dc.subjectLymphoma
dc.subjectMouse model
dc.subjectMetastasis
dc.subjectBone resorption
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectOncology
dc.titleMouse model recapitulates the phenotypic heterogeneity of human adult T-cell leukemia/lymphoma in bone
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication0181d6a3-f0f7-40e3-9dc8-9dc86a78ac8e
relation.isAuthorOfPublication.latestForDiscovery0181d6a3-f0f7-40e3-9dc8-9dc86a78ac8e

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