Micropropogation of Sideritis pisidica Boiss. et Heldr. Apud Bentham

Abstract

Sideritis pisidica Boiss. et Heldr. Apud Bentham is known as a medicinal and aromatic plant. Sideritis pisidica Boiss. et Heldr. Apud Bentham is a plant which is endemic to Turkey and a threatened species in the Labiateae family. This species is used in folk medicine since ancient times. Micropropogation is an alternative method for rapid clonal propogation. In this study, Sideritis pisidica Boiss. et Heldr. Apud Bentham was micropropagated using MS media containing different combinations of plant growth regulators; BA (0, 0.5, 1 and 1.5 mg L-1) and GA3 (0, 0.5, 1 and 1.5 mg L-1) for propagation, IBA (indole-3-butyric acid) 0, 0.5, 1, 1.5 and 2 mg L-1) for rooting. The MS medium supplemented with 0.5 mg L-1 BA and 0.2 mg L-1 GA(3) (giberellic acid) showed highest percentage of shoot multiplication and rooted with 85.5 and 79.5% response from shoot tip explant, respectively.