Remineralization potential of ginger-honey on initial namel lesions of different depth

Abstract

Background: The study aims to explore the remineralization efficacy of toothpaste with the active ingredients of ginger and honey on in-vitro produced early dental caries lesions by using ultrasound, FluoreCam, surface microhardness and light and scanning electron microscope methods. Methods: Total of 105 enamel samples were divided into three main groups which were exposed to a demineralizing solution for 18 (D1), 36 (D2), and 72 (D3) hours to take initial measurements by ultrasound, FluoreCam, and surface microhardness, SEM. All main groups were divided into three subgroups each then, topically applied a toothpaste with NaF content (R1N) and a dental toothpaste with ginger-honey content (R1G) (Gumgumix) by a toothbrush 3x1/day for five minutes and stored in remineralizing solution, measured at the end. Results: Toothpaste with ginger-honey content produced significant remineralization results (p=0.033) on the 52-micron initial enamel lesions (D1) by microhardness. Similarly, the FluoreCam size (p=0.001), microscope (p<0.001) and microhardness (p=0.001) results of the 80-micron lesion distinctly showed remineralization. In the 140-micron lesions (D3), the FluoreCam, microscope, and microhardness marked some changes on demineralization while the ultrasound detected the changes on both the demineralization and remineralization (p=0.035, p=0.025). When the remineralization and demineralization variations were compared, the ultrasound method, along with the microhardness, control, method, appeared to be the only method that differed in all groups in general. In addition, the dental toothpaste with ginger-honey content produced the highest remineralization values. Conclusion: As a result, the application of the dental toothpaste with gingerhoney content produced enhanced remineralization effect in all demineralized groups.