Obtaining dihaploid lines by using anther culture in the different eggplant cultivars

Abstract

This research was carried out in the application greenhouses of the Vegetable Growing Section and Tissue Culture Laboratory of Yalova-Ataturk Horticultural Research Institute. The success rate of embryo formation was found to be significantly higher in the anther culture established with buds obtained from eggplants grown in the summer period. The cultivar "25" gave the best response among the cultivars subjected to anther culture, with embryo and plant formation rates of 14.2% and 5.3%, respectively. After obtaining haploid plants via anther culture, colchicine was applied at concentrations of 0.5% and 1%, for 1 or 2 hours, for obtaining dihaploids under in vitro conditions. The best result was obtained from treatment with 0.5% colchicine for 2 hours and 1% colchicine for 1 hour. Chromosome counts were made in di-haploid plants and seeds were obtained from these plants.