Publication:
Isolation of major phenolic compounds from the extracts of prunella l. Species grown in Turkey and their antioxidant and cytotoxic activities

dc.contributor.buuauthorŞahin, Saliha
dc.contributor.buuauthorArı, Ferda
dc.contributor.buuauthorDemir, Cevdet
dc.contributor.buuauthorUlukaya, Engin
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentFen Edebiyat Fakültesi
dc.contributor.departmentFen Edebiyat Fakültesi
dc.contributor.departmentKimya Bölümü
dc.contributor.departmentBiyoloji Bölümü
dc.contributor.departmentTıbbi Biyokimya Ana Bilim Dalı
dc.contributor.orcid0000-0002-6729-7908
dc.contributor.orcid0000-0003-1508-0181
dc.contributor.orcid0000-0003-1508-0181
dc.contributor.orcid0000-0003-4875-5472
dc.contributor.researcheridAAH-2892-2021
dc.contributor.researcheridAAG-7012-2021
dc.contributor.researcheridABA-2005-2020
dc.contributor.researcheridK-5792-2018
dc.contributor.scopusid15027401600
dc.contributor.scopusid24376085300
dc.contributor.scopusid7003565902
dc.contributor.scopusid6602927353
dc.date.accessioned2024-02-22T07:27:47Z
dc.date.available2024-02-22T07:27:47Z
dc.date.issued2013-06-18
dc.description.abstractThe major phenolics of PrunellaL. species were isolated, and antioxidant activites were determined by 2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid). The isolated compounds were identified as rutin, rosmarinic acid and caffeic acid by high performance liquid chromatography-diode array detection. The results show that the phenolic compounds were isolated from PrunellaL. species with high content ranged from 74% (Prunella grandifloraL.) to 99% (Prunella orientalisBornm.), and the major compound of rosmarinic acid was isolated above 90% in all species. Antigrowth effects of PrunellaL. species were tested against human breast cancer cell lines, MCF-7 (estrogen receptor-positive) and MDAMB-231 (estrogen receptor-negative) by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) and ATP (adenosine triphosphate) viability assays. To determine the mode of cell death, M30-antigen assay was performed in order to measure apoptosis. It was found that Prunella laciniata (L.) L., P.orientalisBornm. and P.grandifloraL. species showed antigrowth effect on MCF-7 and MDA-MB-231 breast cancer cells, but apoptosis was not observed. Practical ApplicationsFour PrunellaL. species (Prunella vulgarisL., Prunella laciniataL., Prunella grandifloraL. and Prunella orientalisBornm.) have been used in Western and Chinese herbal medicine that possesses antioxidant properties. PrunellaL. species have a broad chemical composition, consisting of bioactive molecules as phenolic acids, flavonoids and anthocyanidins. It was found that PrunellaL. species showed a significant growth-inhibiting effect on human breast cancer cells, but apoptosis was not involved in cell death. The results suggest that the isolated rutin and rosmarinic acid, as the principal components of PrunellaL. extracts, could be utilized as both medicine and herbal tea.
dc.identifier.citationŞahin, S. vd. (2013). "Isolation of major phenolic compounds from the extracts of prunella l. Species grown in Turkey and their antioxidant and cytotoxic activities". Journal of Food Biochemistry, 38(2), 248-257.
dc.identifier.doihttps://doi.org/10.1111/jfbc.12043
dc.identifier.eissn1745-4514
dc.identifier.endpage257
dc.identifier.issn0145-8884
dc.identifier.issue2
dc.identifier.scopus2-s2.0-84898002851
dc.identifier.startpage248
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/10.1111/jfbc.12043
dc.identifier.urihttps://hdl.handle.net/11452/39907
dc.identifier.volume38
dc.identifier.wos000334116500013
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherWiley
dc.relation.bap2009/38
dc.relation.journalJournal of Food Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectRosmarinic acid
dc.subjectLamiaceae
dc.subjectGas-chromatography
dc.subjectPrevention
dc.subjectSalvia-officinalis
dc.subjectCapacity
dc.subjectLung-cancer
dc.subjectVulgaris l.
dc.subjectAssay
dc.subjectApoptosis
dc.subjectPrunella laciniata
dc.subjectAdenosinetriphosphate
dc.subjectAssays
dc.subjectHigh performance liquid chromatography
dc.subjectCell culture
dc.subjectCell death
dc.subjectBreast cancer cells
dc.subjectRosmarinic acid
dc.subjectCaffeic acids
dc.subjectCytotoxic activities
dc.subjectHuman breast cancer cells
dc.subjectPhenolic compounds
dc.subjectIsolated compounds
dc.subjectLiquid chromatography-diode array detection
dc.subjectBiochemistry & molecular biology
dc.subjectFood science & technology
dc.subject.scopusExtract; Machaeranthera; Derris
dc.subject.wosBiochemistry & molecular biology
dc.subject.wosFood science & technology
dc.titleIsolation of major phenolic compounds from the extracts of prunella l. Species grown in Turkey and their antioxidant and cytotoxic activities
dc.typeArticle
dc.wos.quartileQ4 (Biochemistry & Molecular Biology)
dc.wos.quartileQ3 (Food Science & Technology)
dspace.entity.typePublication
local.contributor.departmentFen Edebiyat Fakültesi/Kimya Bölümü
local.contributor.departmentFen Edebiyat Fakültesi/Biyoloji Bölümü
local.contributor.departmentTıp Fakültesi/Tıbbi Biyokimya Ana Bilim Dalı
local.indexed.atWOS
local.indexed.atScopus

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