Publication:
The effect of exosomes released from apheresis plateletconcentrates under the impact of gamma irradiation and storage time upon platelet aggregation and hemostasis

dc.contributor.authorBal, Salih Haldun
dc.contributor.authorSağdilek, Engin
dc.contributor.authorKaracay, Mehmet
dc.contributor.authorKızmaz, Muhammed A.
dc.contributor.authorKumaş, Levent T.
dc.contributor.authorCan, Fatma E.
dc.contributor.authorYıldırım, Muzaffer
dc.contributor.authorCanavar-Yıldırım, Tuğçe
dc.contributor.authorKosay-Gülkaya, Deniz
dc.contributor.authorYöyen-Ermiş, Diğdem
dc.contributor.authorBudak, Ferah
dc.contributor.authorHeper, Yasemin
dc.contributor.authorGürsel, İhsan
dc.contributor.authorOral, Haluk B.
dc.contributor.buuauthorBAL, SALİH HALDUN
dc.contributor.buuauthorSAĞDİLEK, ENGİN
dc.contributor.buuauthorKaraçay, Mehmet
dc.contributor.buuauthorKizmaz, Muhammed A.
dc.contributor.buuauthorKumaş, Levent T.
dc.contributor.buuauthorCan, Fatma E.
dc.contributor.buuauthorKosay-Gülkaya, Deniz
dc.contributor.buuauthorYÖYEN ERMİŞ, DİĞDEM
dc.contributor.buuauthorBUDAK, FERAH
dc.contributor.buuauthorHEPER, YASEMİN
dc.contributor.buuauthorORAL, HALUK BARBAROS
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Dr. Raşit Durusoy Kan Bankası.
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Biyofizik Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Tıp-İmmünoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Mikrobiyoloji-İmmünoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Biyoistatistik Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı.
dc.contributor.orcid0000-0001-7625-9148
dc.contributor.orcid0000-0001-5871-8769
dc.contributor.orcid0000-0001-5334-7911
dc.contributor.orcid0000-0002-1953-7735
dc.contributor.orcid0000-0002-1953-7735
dc.contributor.researcheridHJY-9001-2023
dc.contributor.researcheridAAH-4397-2021
dc.contributor.researcheridJHB-7829-2023
dc.contributor.researcheridHKN-2347-2023
dc.contributor.researcheridFHB-1791-2022
dc.contributor.researcheridJSL-7718-2023
dc.contributor.researcheridHJH-5312-2023
dc.contributor.researcheridHJH-5217-2023
dc.contributor.researcheridIYU-6844-2023
dc.contributor.researcheridAAH-3888-2021
dc.contributor.researcheridIZP-9398-2023
dc.contributor.researcheridHOK-0035-2023
dc.contributor.researcheridK-7285-2012
dc.date.accessioned2024-11-08T11:12:21Z
dc.date.available2024-11-08T11:12:21Z
dc.date.issued2023-05-01
dc.description.abstractBackground -Blood components should be gamma-irradiated (& gamma;-IR) in order to prevent transfusion-associated graft-versus-host disease. The aim of this study is to determine the effect of & Gamma;-IR and storage time on the exosomes released from apheresis platelet concentrates (aPC) and to investigate their impact on the maximum platelet aggregation (MPA) and hemostasis. Materials and methods -Eight units of aPC were included in this study. These were divided into four equal portions. Two portions were irradiated before storage while the other two were not. Thus, irradiated and non-irradiated aPC samples for storage Days 0 (D0) and 5 (D5) were obtained. Exosomes were isolated from these samples using a commercial kit and were evaluated to ascertain their parent cells by flow cytometry. For the following steps, exosomes were pooled according to their features. Pooled exosomes were then used for aggregometry and thromboelastography. Results -Platelet-derived exosome (PD-EX) levels decreased in D5 compared to D0 in NI-aPC, whereas granulocyte-derived exosome (GD-EX) levels increased. Exosome pools had no effect on MPA compared to saline groups. Exosome pools decreased the time to initial fibrin formation (R), whereas they increased the rate of clot formation (& alpha;-angle) and coagulation index (CI) compared to saline groups. Discussion -Storage time and & Gamma;-IR each have almost the opposite effects on PD-EX and GD-EX. Exosomes have no impact on MPA, but enhance the clot strength. The impact of exosomes on aPC quality and effectiveness can be ignored or considered as a positive effect.
dc.description.sponsorshipEuropean Cooperation in Science and Technology (COST) BM1404
dc.description.sponsorshipEU Framework Program Horizon 2020
dc.description.sponsorshipTürkiye Ulusal Kan Bankacılığı ve Transfüzyon Tıbbı Kongresi
dc.identifier.doi10.2450/2022.006-22
dc.identifier.endpage267
dc.identifier.issn1723-2007
dc.identifier.issue3
dc.identifier.startpage257
dc.identifier.urihttps://doi.org/10.2450/2022.006-22
dc.identifier.urihttps://www.bloodtransfusion.it/bt/article/view/377
dc.identifier.urihttps://hdl.handle.net/11452/47629
dc.identifier.volume21
dc.identifier.wos001026246000011
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherSimtipro Srl
dc.relation.bapTHIZ-2021-739
dc.relation.journalBlood Transfusion
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectExtracellular vesicles
dc.subjectQuality-control
dc.subjectHuman plasma
dc.subjectMicroparticles
dc.subjectInactivation
dc.subjectActivation
dc.subjectRadiation
dc.subjectAggregation
dc.subjectExosome
dc.subjectGamma irradiation
dc.subjectHemostasis
dc.subjectPlatelet concentrates
dc.subjectHematology
dc.titleThe effect of exosomes released from apheresis plateletconcentrates under the impact of gamma irradiation and storage time upon platelet aggregation and hemostasis
dc.typeArticle
dspace.entity.typePublication
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