Publication: The effect of exosomes released from apheresis plateletconcentrates under the impact of gamma irradiation and storage time upon platelet aggregation and hemostasis
dc.contributor.author | Bal, Salih Haldun | |
dc.contributor.author | Sağdilek, Engin | |
dc.contributor.author | Karacay, Mehmet | |
dc.contributor.author | Kızmaz, Muhammed A. | |
dc.contributor.author | Kumaş, Levent T. | |
dc.contributor.author | Can, Fatma E. | |
dc.contributor.author | Yıldırım, Muzaffer | |
dc.contributor.author | Canavar-Yıldırım, Tuğçe | |
dc.contributor.author | Kosay-Gülkaya, Deniz | |
dc.contributor.author | Yöyen-Ermiş, Diğdem | |
dc.contributor.author | Budak, Ferah | |
dc.contributor.author | Heper, Yasemin | |
dc.contributor.author | Gürsel, İhsan | |
dc.contributor.author | Oral, Haluk B. | |
dc.contributor.buuauthor | BAL, SALİH HALDUN | |
dc.contributor.buuauthor | SAĞDİLEK, ENGİN | |
dc.contributor.buuauthor | Karaçay, Mehmet | |
dc.contributor.buuauthor | Kizmaz, Muhammed A. | |
dc.contributor.buuauthor | Kumaş, Levent T. | |
dc.contributor.buuauthor | Can, Fatma E. | |
dc.contributor.buuauthor | Kosay-Gülkaya, Deniz | |
dc.contributor.buuauthor | YÖYEN ERMİŞ, DİĞDEM | |
dc.contributor.buuauthor | BUDAK, FERAH | |
dc.contributor.buuauthor | HEPER, YASEMİN | |
dc.contributor.buuauthor | ORAL, HALUK BARBAROS | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Dr. Raşit Durusoy Kan Bankası. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyofizik Anabilim Dalı. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Tıp-İmmünoloji Anabilim Dalı. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Mikrobiyoloji-İmmünoloji Anabilim Dalı. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Biyoistatistik Anabilim Dalı. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı. | |
dc.contributor.orcid | 0000-0001-7625-9148 | |
dc.contributor.orcid | 0000-0001-5871-8769 | |
dc.contributor.orcid | 0000-0001-5334-7911 | |
dc.contributor.orcid | 0000-0002-1953-7735 | |
dc.contributor.orcid | 0000-0002-1953-7735 | |
dc.contributor.researcherid | HJY-9001-2023 | |
dc.contributor.researcherid | AAH-4397-2021 | |
dc.contributor.researcherid | JHB-7829-2023 | |
dc.contributor.researcherid | HKN-2347-2023 | |
dc.contributor.researcherid | FHB-1791-2022 | |
dc.contributor.researcherid | JSL-7718-2023 | |
dc.contributor.researcherid | HJH-5312-2023 | |
dc.contributor.researcherid | HJH-5217-2023 | |
dc.contributor.researcherid | IYU-6844-2023 | |
dc.contributor.researcherid | AAH-3888-2021 | |
dc.contributor.researcherid | IZP-9398-2023 | |
dc.contributor.researcherid | HOK-0035-2023 | |
dc.contributor.researcherid | K-7285-2012 | |
dc.date.accessioned | 2024-11-08T11:12:21Z | |
dc.date.available | 2024-11-08T11:12:21Z | |
dc.date.issued | 2023-05-01 | |
dc.description.abstract | Background -Blood components should be gamma-irradiated (& gamma;-IR) in order to prevent transfusion-associated graft-versus-host disease. The aim of this study is to determine the effect of & Gamma;-IR and storage time on the exosomes released from apheresis platelet concentrates (aPC) and to investigate their impact on the maximum platelet aggregation (MPA) and hemostasis. Materials and methods -Eight units of aPC were included in this study. These were divided into four equal portions. Two portions were irradiated before storage while the other two were not. Thus, irradiated and non-irradiated aPC samples for storage Days 0 (D0) and 5 (D5) were obtained. Exosomes were isolated from these samples using a commercial kit and were evaluated to ascertain their parent cells by flow cytometry. For the following steps, exosomes were pooled according to their features. Pooled exosomes were then used for aggregometry and thromboelastography. Results -Platelet-derived exosome (PD-EX) levels decreased in D5 compared to D0 in NI-aPC, whereas granulocyte-derived exosome (GD-EX) levels increased. Exosome pools had no effect on MPA compared to saline groups. Exosome pools decreased the time to initial fibrin formation (R), whereas they increased the rate of clot formation (& alpha;-angle) and coagulation index (CI) compared to saline groups. Discussion -Storage time and & Gamma;-IR each have almost the opposite effects on PD-EX and GD-EX. Exosomes have no impact on MPA, but enhance the clot strength. The impact of exosomes on aPC quality and effectiveness can be ignored or considered as a positive effect. | |
dc.description.sponsorship | European Cooperation in Science and Technology (COST) BM1404 | |
dc.description.sponsorship | EU Framework Program Horizon 2020 | |
dc.description.sponsorship | Türkiye Ulusal Kan Bankacılığı ve Transfüzyon Tıbbı Kongresi | |
dc.identifier.doi | 10.2450/2022.006-22 | |
dc.identifier.endpage | 267 | |
dc.identifier.issn | 1723-2007 | |
dc.identifier.issue | 3 | |
dc.identifier.startpage | 257 | |
dc.identifier.uri | https://doi.org/10.2450/2022.006-22 | |
dc.identifier.uri | https://www.bloodtransfusion.it/bt/article/view/377 | |
dc.identifier.uri | https://hdl.handle.net/11452/47629 | |
dc.identifier.volume | 21 | |
dc.identifier.wos | 001026246000011 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Simtipro Srl | |
dc.relation.bap | THIZ-2021-739 | |
dc.relation.journal | Blood Transfusion | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Extracellular vesicles | |
dc.subject | Quality-control | |
dc.subject | Human plasma | |
dc.subject | Microparticles | |
dc.subject | Inactivation | |
dc.subject | Activation | |
dc.subject | Radiation | |
dc.subject | Aggregation | |
dc.subject | Exosome | |
dc.subject | Gamma irradiation | |
dc.subject | Hemostasis | |
dc.subject | Platelet concentrates | |
dc.subject | Hematology | |
dc.title | The effect of exosomes released from apheresis plateletconcentrates under the impact of gamma irradiation and storage time upon platelet aggregation and hemostasis | |
dc.type | Article | |
dspace.entity.type | Publication | |
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