Publication:
Brucella abortus L7/L12 recombinant protein induces strong Th1 response in acute brucellosis patients

dc.contributor.authorOliveira, Sergio
dc.contributor.buuauthorKazak, Esra
dc.contributor.buuauthorGöral, Güher
dc.contributor.buuauthorAkalın, Halis
dc.contributor.buuauthorYılmaz, Emel
dc.contributor.buuauthorHeper, Yasemin
dc.contributor.buuauthorOral, Haluk Barbaros
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentTıbbi Mikrobiyoloji Ana Bilim Dalı
dc.contributor.departmentEnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Ana Bilim Dalı
dc.contributor.orcid0000-0003-0463-6818
dc.contributor.researcheridK-7285-2012
dc.contributor.researcheridAAG-8459-2021
dc.contributor.researcheridAAH-6506-2021
dc.contributor.researcheridAAU-8952-2020
dc.contributor.scopusid24921238200
dc.contributor.scopusid6603453166
dc.contributor.scopusid57207553671
dc.contributor.scopusid22037135100
dc.contributor.scopusid56191003300
dc.contributor.scopusid7004498001
dc.date.accessioned2022-04-15T13:05:35Z
dc.date.available2022-04-15T13:05:35Z
dc.date.issued2010-09
dc.description.abstractBackground: Because of high morbidity of the brucellosis in humans and the potential use of the microorganism as an agent of biologic warfare, protection of effective vaccines and specific diagnostic reagents become necessary to eradicate brucellosis. Objective: In this study we aimed to investigate the cytokine responses and changes in peripheral blood lymphocyte subgroups of acute brucellosis patients in response to L7/L12 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) recombinant proteins derived from Brucella abortus. Methods: levels of IFN-gamma, IL-4 and IL-10 secreted from PBMCs of 25 acute brucellosis patients and 15 healthy controls, stimulated with Phytohemagglutinin (PHA), L7/L12 or GAPDH were measured by ELISA. Furthermore alterations in lymphocyte subgroups in response to these Brucella antigens were determined by flow cytometry. Results: Extracellular IFN-gamma levels were found to be elevated after stimulation with L7/L12 in patients with acute brucellosis, whereas no significant changes were found in IL-4 and IL-10 levels. Similar data was also obtained with GAPDH, but the stimulation of IFN-gamma production was not observed in all patients and was not as strong as that observed for L7/L12. Moreover, when the distribution of lymphocytes subgroups (CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), CD4(+)CD25(+), CD3(+)CD69(+) and CD3(+)CD152(+)) was evaluated, it was found that the stimulation with L7/L12 and GAPDH only led to an increase in the percentage of CD3(+)CD69(+) lymphocytes. Conclusion: These data indicate that Brucella abortus L7/L12 or GAPDH induce a Th1 type immune response in acute brucellosis patients. Additionally, these recombinant proteins, especially L7/L12, may be used in new vaccine preparations and diagnostic tests.
dc.identifier.citationKazak, E. vd. (2010). "Brucella abortus L7/L12 recombinant protein induces strong Th1 response in acute brucellosis patients". Iranian Journal of Immunology, 7(3), 132-141.
dc.identifier.endpage141
dc.identifier.issn1735-1383
dc.identifier.issn1735-367X
dc.identifier.issue3
dc.identifier.pubmed20876984
dc.identifier.scopus2-s2.0-79952666425
dc.identifier.startpage132
dc.identifier.urihttps://iji.sums.ac.ir/article_17050_0.html
dc.identifier.urihttp://hdl.handle.net/11452/25810
dc.identifier.volume7
dc.identifier.wos000208406400001
dc.indexed.scopusScopus
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherShiraz Institute of Cancer Research
dc.relation.collaborationYurt dışı
dc.relation.journalIranian Journal of Immunology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBrucellosis
dc.subjectCytokine
dc.subjectRecombinant proteins
dc.subjectT lymphocytes
dc.subjectImmunology
dc.subject.emtreeGamma interferon
dc.subject.emtreeGlyceraldehyde 3 phosphate dehydrogenase
dc.subject.emtreeInterleukin 10
dc.subject.emtreeInterleukin 4
dc.subject.emtreeRecombinant protein
dc.subject.emtreeRecombinant protein L7 L12
dc.subject.emtreeUnclassified drug
dc.subject.emtreeAdult
dc.subject.emtreeArticle
dc.subject.emtreeBrucella abortus
dc.subject.emtreeBrucellosis
dc.subject.emtreeCD3+ T lymphocyte
dc.subject.emtreeCD4+ CD25+ T lymphocyte
dc.subject.emtreeCD4+ T lymphocyte
dc.subject.emtreeCD8+ T lymphocyte
dc.subject.emtreeCellular distribution
dc.subject.emtreeCellular immunity
dc.subject.emtreeClinical article
dc.subject.emtreeControlled study
dc.subject.emtreeCytokine release
dc.subject.emtreeEnzyme linked immunosorbent assay
dc.subject.emtreeFemale
dc.subject.emtreeFlow cytometry
dc.subject.emtreeHuman
dc.subject.emtreeHuman cell
dc.subject.emtreeLymphocyte
dc.subject.emtreeMale
dc.subject.emtreePeripheral lymphocyte
dc.subject.emtreeProtein blood level
dc.subject.emtreeSerology
dc.subject.emtreeTh1 cell
dc.subject.meshAdult
dc.subject.meshBrucella abortus
dc.subject.meshBrucellosis
dc.subject.meshCase-control studies
dc.subject.meshCytokines
dc.subject.meshFemale
dc.subject.meshGlyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
dc.subject.meshHumans
dc.subject.meshLeukocytes, mononuclear
dc.subject.meshLymphocyte subsets
dc.subject.meshMale
dc.subject.meshMiddle aged
dc.subject.meshRecombinant proteins
dc.subject.meshRibosomal proteins
dc.subject.meshTh1 cells
dc.subject.scopusBrucella Abortus; Zoonosis; Bovine Brucellosis
dc.subject.wosImmunology
dc.titleBrucella abortus L7/L12 recombinant protein induces strong Th1 response in acute brucellosis patients
dc.typeArticle
dc.wos.quartileN/A
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Ana Bilim Dalı
local.contributor.departmentTıp Fakültesi/Tıbbi Mikrobiyoloji Ana Bilim Dalı
local.indexed.atPubMed
local.indexed.atWOS
local.indexed.atScopus

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