Publication: A rational approach for 3d recognition and removal of l-asparagine via molecularly imprinted membranes
dc.contributor.author | Acet, Ömür | |
dc.contributor.author | Acet, Burcu Onal | |
dc.contributor.author | Dikici, Emrah | |
dc.contributor.author | Odabası, Mehmet | |
dc.contributor.buuauthor | NOMA, Samir Abbas Ali | |
dc.contributor.buuauthor | Noma, Samir Abbas Ali | |
dc.contributor.buuauthor | OSMAN, BİLGEN | |
dc.contributor.buuauthor | Osman, Bilgen | |
dc.contributor.department | Fen Edebiyat Fakültesi | |
dc.contributor.department | Kimya Ana Bilim Dalı | |
dc.contributor.orcid | 0000-0001-8406-149X | |
dc.contributor.researcherid | ABF-4791-2020 | |
dc.contributor.researcherid | ABH-1773-2021 | |
dc.date.accessioned | 2024-11-27T05:30:15Z | |
dc.date.available | 2024-11-27T05:30:15Z | |
dc.date.issued | 2023-01-17 | |
dc.description.abstract | In this study, a L-asparagine (L-Asn) imprinted membranes (L-Asn-MIPs) were synthesized via molecular imprinting for selective and efficient removal of L-Asn. The L-Asn-MIP membrane was prepared by using acrylamide (AAm) and hydroxyethyl methacrylate (HEMA) as a functional monomer and a comonomer, respectively. The membrane was characterized by scanning electron microscopy (SEM) and Fourier Transform infrared spectroscopy (FTIR). The L-Asn adsorption capacity of the membrane was investigated in detail. The maximum L-Asn adsorption capacity was determined as 408.2 mg/g at pH: 7.2, 24 degrees C. Determination of L-Asn binding behaviors of L-Asn-MIPs also shown with Scatchard analyses. The effect of pH on L-Asn adsorption onto the membrane and also the selectivity and reusability of the L-Asn-MIPs for L-Asn adsorption were determined through L-asparaginase (L-ASNase) enzyme activity measurements. The selectivity of the membrane was investigated by using two different ternary mixtures; L-glycine (L-Gly)/L-histidine (L-His)/L-Asn and L-tyrosin (L-Tyr)/L-cystein(L-Cys)/L-Asn. The obtained results showed that the L-Asn-MIP membranes have a high selectivity towards L-Asn. | |
dc.identifier.doi | 10.1016/j.jpba.2023.115250 | |
dc.identifier.issn | 0731-7085 | |
dc.identifier.uri | https://doi.org/10.1016/j.jpba.2023.115250 | |
dc.identifier.uri | https://hdl.handle.net/11452/48535 | |
dc.identifier.volume | 226 | |
dc.identifier.wos | 000971451800001 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Elsevier | |
dc.relation.journal | Journal Of Pharmaceutical And Biomedical Analysis | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Acrylamide | |
dc.subject | Agent | |
dc.subject | Asparagine | |
dc.subject | L-asparaginase activity | |
dc.subject | Molecular imprinting | |
dc.subject | Removal | |
dc.subject | Science & technology | |
dc.subject | Physical sciences | |
dc.subject | Life sciences & biomedicine | |
dc.subject | Chemistry, analytical | |
dc.subject | Pharmacology & pharmacy | |
dc.subject | Chemistry | |
dc.title | A rational approach for 3d recognition and removal of l-asparagine via molecularly imprinted membranes | |
dc.type | Article | |
dspace.entity.type | Publication | |
local.contributor.department | Fen Edebiyat Fakültesi/Kimya Ana Bilim Dalı | |
relation.isAuthorOfPublication | 08ae8d1b-5dad-4ab3-8186-7723e086d163 | |
relation.isAuthorOfPublication | b696c48b-ee92-4822-be1f-c84f4fe0e3a6 | |
relation.isAuthorOfPublication.latestForDiscovery | b696c48b-ee92-4822-be1f-c84f4fe0e3a6 |