Publication:
Molecular identification and determination of some virulence genes of Aeromonas spp. in fish and water from Turkish coastal regions

dc.contributor.authorOnuk, Ertan Emek
dc.contributor.authorFındık, Arzu
dc.contributor.authorTürk, Necla
dc.contributor.authorKorun, Jale
dc.contributor.authorÖzer, Selmin
dc.contributor.authorAvsever, Meriç Lütfi
dc.contributor.authorÇiftçi, Alper
dc.contributor.buuauthorAltun, Soner
dc.contributor.departmentVeterinerlik Fakültesi
dc.contributor.departmentSu Ürünleri Hastalıkları Ana Bilim Dalı
dc.contributor.orcid0000-0001-9096-875X
dc.contributor.researcheridAAG-8518-2021
dc.contributor.scopusid56269221600
dc.date.accessioned2023-01-06T07:29:20Z
dc.date.available2023-01-06T07:29:20Z
dc.date.issued2013-04
dc.description.abstractIdentification of Aeromonas species is known to be troublesome due to their phenotypic and genotypic heterogeneity. A total of 60 bacterial isolates from water and rainbow trout samples from Turkish coastal regions (Black Sea, Aegean Sea and Mediterranean Sea) were used for 16S rDNA-Restriction Fragment Length Polymorphisms (RFLP) analysis with AluI, MboI, NarI and PstI as restriction enzymes and molecular identification was eventually completed with PCR amplification of the fstA, A. salmonicida specific gene. In addition, the presence of 6 virulence genes (aer, ser, lip, gcat, nuc and laf) in the isolates was investigated using rapid hexaplex-PCR. The frequencies of the identified Aeromonas species were 38.33% for A. sobria, 23.33% for A. hydrophila, 10.0% for A. veronii and 26.67% for A. salmonicida and only one strain was not clearly identified. The more frequent virulence genes were aer, ser, lip and gcat found in 66.66%, 61.67%, 50% and 33.33% of isolates, respectively and 14 different virulence profiles were determined. This study shows that the 16S rDNA RFLP analysis combined with fstA gene specific PCR was useful for rapid and reliable identification of Aeromonas strains and the coupled determination of the virulence genes may help to both control fish disease arising from Aeromonas spp. and alert human health risk.
dc.description.sponsorshipOndokuz Mayis Üniversitesi (VET 051)
dc.identifier.citationOnuk, E. E. vd. (2013). "Molecular identification and determination of some virulence genes of Aeromonas spp. in fish and water from Turkish coastal regions". Revue de Medecine Veterinaire, 164(4), 200-206.
dc.identifier.endpage206
dc.identifier.issn0035-1555
dc.identifier.issn2258-0646
dc.identifier.issue4
dc.identifier.scopus2-s2.0-84875724274
dc.identifier.startpage200
dc.identifier.urihttp://hdl.handle.net/11452/30299
dc.identifier.volume164
dc.identifier.wos000334093200006
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherEcole Nationale Veterinaire Toulouse
dc.relation.collaborationYurt içi
dc.relation.collaborationSanayi
dc.relation.journalRevue de Medecine Veterinaire
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectVeterinary sciences
dc.subjectAeromonas
dc.subjectFish
dc.subjectRFLP
dc.subjectRestriction enzymes
dc.subjectVirulence genes
dc.subjectTurkish coastal regions
dc.subjectFragment-length-polymorphism
dc.subjectHydrophila
dc.subjectMembers
dc.subjectAdherence
dc.subjectCaviae
dc.subjectAeromonas
dc.subjectAeromonas hydrophila
dc.subjectAeromonas salmonicida
dc.subjectAeromonas sobria
dc.subjectAeromonas veronii
dc.subjectBacteria (microorganisms)
dc.subjectHexaplex
dc.subjectOncorhynchus mykiss
dc.subject.scopusAeromonas; Aeromonas Hydrophila; Virulence
dc.subject.wosVeterinary sciences
dc.titleMolecular identification and determination of some virulence genes of Aeromonas spp. in fish and water from Turkish coastal regions
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentVeterinerlik Fakültesi/Su Ürünleri Hastalıkları Ana Bilim Dalı
local.indexed.atScopus
local.indexed.atWOS

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