Effect of ovariohysterectomy on lipid peroxidation and levels of some antioxidants and biochemical parameters in bitches

Abstract

The aim of the presented study was to investigate the effect of ovariohysterectomy on lipid peroxidation and levels of some antioxidants and blood parameters in bitches. Thirty non-pregnant healthy bitches (1-3 years of age) were used in the study. All bitches were anaesthetised with xylazine-ketamine. Levels of glucose, total protein, calcium, inorganic phosphorus (IP), alanine amino transferase (ALT), aspartate amino transferase (AST), glutathione (GSH), and malondialdehyde (MDA) were determined in pre-operative and post-operative bitches. The statistical differences were found in the GSH and MDA levels between the pre- and post-operation animals. There were no significant differences of other biochemical parameters. The results of this study showed that the determination of alterations in the GSH and MDA levels could be useful for evaluation of the antioxidant defence mechanism after ovariohysterectomy in bitches.