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The MTT assay yields a relatively lower result of growth inhibition than the ATP assay depending on the chemotherapeutic drugs tested

dc.contributor.buuauthorUlukaya, Engin
dc.contributor.buuauthorÖzdikiçioglu, Ferda
dc.contributor.buuauthorYılmaztepe, Arzu
dc.contributor.buuauthorDemirci, Meral
dc.contributor.departmentFen Edebiyat Fakültesi
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentBiyoloji Bölümü
dc.contributor.departmentBiyokimya ve Klinik Kimya Ana Bilim Dalı
dc.contributor.orcid0000-0002-6729-7908
dc.contributor.orcid0000-0003-0463-6818
dc.contributor.researcheridAAG-7012-2021
dc.contributor.researcheridA-5841-2017
dc.contributor.researcheridK-5792-2018
dc.contributor.scopusid6602927353
dc.contributor.scopusid6504081459
dc.contributor.scopusid23091316500
dc.contributor.scopusid18534643800
dc.date.accessioned2021-10-22T09:01:13Z
dc.date.available2021-10-22T09:01:13Z
dc.date.issued2008-02
dc.description.abstractAccurate assessment of the anti-growth effects of chemotherapeutics is immensely importance in cancer research with regard to drug discovery and toxicological safety. A number of in vitro cytotoxicity assays are used for these purposes. However, there is the possibility for different results in the assessments because the way they measure the viability of cancer cells is specific to each assay. In the present study, the performance of two common assays (MTT and ATP) in the assessment of anti-growth effects of chemotherapeutics on a lung cancer cell line (A549) was compared. The cells were treated with paclitaxel, docetaxel, gemcitabine, 5-fluorouracil (5-FU), etoposide, doxorubicin, epirubicin, cisplatin, 4-hydroperoxycyclophosphamide (4-HC) and carboplatin in six different concentrations. When taking all the drugs and inhibitions into account, a moderate correlation (r = 0.671); p = 0.01) between the assays was found. However, IC 50 values by the MTT assay were higher in 90% of the drugs than those found by the ATP assay. In addition to this, there was a statistically significant difference between the dose response curves of the assays, which was dependent on the drugs of choice. We recommend caution in comparing these assays to evaluate the anti-growth effects of chemotherapeutics because the MTT assay seem to give rise to relatively lower inhibition (higher viability) levels than the ATP assay, depending on the drugs of choice.
dc.identifier.citationUlukaya, E. vd. (2008). ''The MTT assay yields a relatively lower result of growth inhibition than the ATP assay depending on the chemotherapeutic drugs tested''. Toxicology in Vitro, 22(1), 232-239.
dc.identifier.endpage239
dc.identifier.issn08872333
dc.identifier.issue1
dc.identifier.pubmed17904330
dc.identifier.scopus2-s2.0-37249010159
dc.identifier.startpage232
dc.identifier.urihttps://doi.org/10.1016/j.tiv.2007.08.006
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0887233307002287
dc.identifier.urihttp://hdl.handle.net/11452/22434
dc.identifier.volume22
dc.identifier.wos000252907100027
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherPergamon-Elsevier Science
dc.relation.bapBAP
dc.relation.collaborationYurt içi
dc.relation.journalToxicology in Vitro
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMTT assay
dc.subjectATP assay
dc.subjectChemotherapeutics
dc.subjectCyrotoxicity
dc.subjectIn vitro
dc.subjectA549
dc.subjectLuminescence assay
dc.subjectOvarian-cancer
dc.subjectChemosensitivity assay
dc.subjectToxiciy assays
dc.subjectCell-lines
dc.subjectIn-vitro
dc.subjectReduction
dc.subjectApoptosis
dc.subjectSurvival
dc.subjectCytotoxicity
dc.subject.emtree3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
dc.subject.emtree4 hydroperoxycyclophosphamide
dc.subject.emtreeAdenosine triphosphate
dc.subject.emtreeAntineoplastic agent
dc.subject.emtreeCarboplatin
dc.subject.emtreeCisplatin
dc.subject.emtreeDocetaxel
dc.subject.emtreeDoxorubicin
dc.subject.emtreeEpirubicin
dc.subject.emtreeEtoposide
dc.subject.emtreeFluorouracil
dc.subject.emtreeGemcitabine
dc.subject.emtreeArticle
dc.subject.emtreeCancer cell culture
dc.subject.emtreeCell viability
dc.subject.emtreeControlled study
dc.subject.emtreeCytotoxicity
dc.subject.emtreeGrowth inhibition
dc.subject.emtreeHuman
dc.subject.emtreeHuman cell
dc.subject.emtreeIC 50
dc.subject.emtreeIntermethod comparison
dc.subject.emtreeToxicity testing
dc.subject.meshAdenosine triphosphate
dc.subject.meshAntineoplastic agents
dc.subject.meshCell line, tumor
dc.subject.meshCell survival
dc.subject.meshDose-response relationship drug
dc.subject.meshDrug screening assays, antitumor
dc.subject.meshHumans
dc.subject.meshInhibitory concentration 50
dc.subject.meshLung neoplasms
dc.subject.meshReproducibility of results
dc.subject.meshTetrazolium salts
dc.subject.meshThiazoles
dc.subject.scopusOvary carcinoma; Carboplatin; Irinotecan
dc.subject.wosToxicology
dc.titleThe MTT assay yields a relatively lower result of growth inhibition than the ATP assay depending on the chemotherapeutic drugs tested
dc.typeArticle
dc.wos.quartileQ2
dspace.entity.typePublication
local.contributor.departmentFen Edebiyat Fakültesi/Biyoloji Bölümü
local.contributor.departmentTıp Fakültesi/Biyokimya ve Klinik Kimya Ana Bilim Dalı
local.indexed.atPubMed
local.indexed.atWOS

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