The effects of lactated ringer's resuscitation on tissue blood flows and oxidant injury in experimental crush injury in rats

Abstract

BACKGROUND: In an experimental model of crush injury, tissue blood flow, the extend of oxidant injury and the effect of lactated ringer's resuscitation were investigated. MATERIAL AND METHOD: Rats were divided into sham (n: 8), crush injury (n: 8), and crush injury + lactated ringer's resuscitation (n: 8) groups. Arterial and venous catheterization were performed in all groups. Crush injury was done with intravenous infusion of allogenic muscle extract. In the crush injury + lactated ringer's resuscitation group 30 ml/kg lactated ringer's solution was infused in 30 minutes. Kidney and liver blood flow were measured by using a laser flowmeter. To assess the oxidant injury blood, liver, and kidney tissue samples were collected for malondialdehyde and glutathione measurements. RESULTS: In the crush injury, diminished liver and kidney blood flow rates were improved with lactated ringer's resuscitation. In addition, glutathione levels decreased whereas malondialdehyde levels and base deficit increased. Lactated ringer's resuscitation brought base deficit to the control levels. When compared with the crush injury, lactated ringer's infusion increased the glutathione levels but could not decrease the malondialdehyde ones. CONCLUSION: Lactated ringer's resuscitation improved the blood flow rates but could not prevent oxidant injury totally.