Publication: Detection of israel variant 2 (is/1494/06) genotype of infectious bronchitis virus in a layer chicken flock
dc.contributor.author | Ongor, Hasan | |
dc.contributor.author | Timurkaan, Necati | |
dc.contributor.author | Coven, Fethiye | |
dc.contributor.author | Karabulut, Burak | |
dc.contributor.author | Eroksuz, Hatice | |
dc.contributor.author | Cetinkaya, Burhan | |
dc.contributor.buuauthor | Çarlı, Kamil Tayfun | |
dc.contributor.buuauthor | CARLI, KAMİL TAYFUN | |
dc.contributor.department | Veteriner Fakültesi | |
dc.contributor.department | Mikrobiyoloji Ana Bilim Dalı | |
dc.contributor.orcid | 0000-0001-6045-8644 | |
dc.date.accessioned | 2024-06-04T11:21:06Z | |
dc.date.available | 2024-06-04T11:21:06Z | |
dc.date.issued | 2021-01-01 | |
dc.description.abstract | The aim of this study was to determine an infectious bronchitis (IB) infection, caused by an Israel variant 2 (IS/1494/06)-like IBV, in a layer chicken flock regularly vaccinated with vaccines containing IBV H120 and 4/91 strains. Mild respiratory symptoms, drop in egg production and soft-shelled eggs and eventually death were observed in a layer chicken flock. Clinical samples from four diseased chickens were examined for the detection and genotyping of IBV by virus isolation, a commercial real time reverse transcription polymerase chain reaction (rRT-PCR) and nucleotide sequencing. Both Israel variant 2 (IS-Var2) and 793/B serotypes were detected from samples by rRT-PCR, but sequencing results of a 345 bp part of S1 gene revealed that our IBV isolate, HFT-IBV, was IS/1494/06 (IS-Var2)-like with the 97.7% genetic similarity. These results suggested that immunity against vaccination with a combination of different genotypes (H120 and 4/91) could not be protective for IS-Var2 IBV field infection. In addition, identification of genotypes from the clinical samples, such as swabs and organ samples by commercial rRT-PCR assays failed to find correct IBV genotype responsible for the IB infection, whereas analysis of nucleotide sequencing of S1 gene of IBV as a gold standard test undoubtedly detected causative genotype of the infection. Also, the findings indicated that there is an urgent need for consider genotype- or protectotype-match vaccination strategies in the field to prevent vaccine- and IB-dependent economic losses of the poultry sector and logically protect chickens from IBV infection. | |
dc.identifier.doi | 10.33988/auvfd.756970 | |
dc.identifier.endpage | 172 | |
dc.identifier.issn | 1300-0861 | |
dc.identifier.issue | 2 | |
dc.identifier.startpage | 167 | |
dc.identifier.uri | https://doi.org/10.33988/auvfd.756970 | |
dc.identifier.uri | https://hdl.handle.net/11452/41725 | |
dc.identifier.volume | 68 | |
dc.identifier.wos | 000636225100008 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Ankara Universitesi | |
dc.relation.journal | Ankara Universitesi Veteriner Fakultesi Dergisi | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Phylogeny | |
dc.subject | Genotype | |
dc.subject | Infectious bronchitis virus | |
dc.subject | Israel variant 2 | |
dc.subject | Layer chicken | |
dc.subject | Vaccination | |
dc.subject | Science & technology | |
dc.subject | Life sciences & biomedicine | |
dc.subject | Veterinary sciences | |
dc.subject | Veterinary sciences | |
dc.title | Detection of israel variant 2 (is/1494/06) genotype of infectious bronchitis virus in a layer chicken flock | |
dc.type | Article | |
dspace.entity.type | Publication | |
local.contributor.department | Veteriner Fakültesi/Mikrobiyoloji Ana Bilim Dalı | |
relation.isAuthorOfPublication | cd431f1b-601a-401d-b8e4-f4b7c734ec85 | |
relation.isAuthorOfPublication.latestForDiscovery | cd431f1b-601a-401d-b8e4-f4b7c734ec85 |
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