2015 Cilt 9 Sayı 25
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Item Open Access Biological denitrification using pure cultures isolated from wastewater treatment plant of Khenchela (Eastern Algeria)(Uludağ Üniversitesi, 2015-04-17) Naili, Oumaima; Benounis, Messaoud; Darbouche, Abdelhak; Kheddouma, AsmaThe purpose of this study was to investigate biological dentirification of wastewater by pure cultures isolated from activated sludge of wastewater treatment plant of Khenchela (Eastern Algeria). Experiments were performed in batch reactor under anaerobic conditions. Three pure strains were isolated and identified as Enterobacter cloacae, Pseudomonas luteola and Aeromonas hydrophila Three carbon sources were used in this work; glucose, methanol and lactose. High denitrification was obtained at 2000 ppm for different carbon sources. Kinetic of bacterial growth and denitrification were studied. We show that bacterial growth rate directly influences nitrate removal from the medium and the use of glucose as carbon source provide high denitrification rate than methanol and lactose. These strains isolated from activated sludge will be used to develop new biosensors applied to the detection and determination of nitrate in real water.Item Open Access Influence of various volatile oils as a dietary supplement on biochemical and performance parameters in broilers(Uludağ Üniversitesi, 2015-04-28) Belenli, Deniz; Udum, Duygu; Cengiz, S. Şule; Polat, Ümit; Uludağ Üniversitesi/Veteriner Fakültesi.Volatile oils like rosemary, thyme and fennel oils have positive effects on poultry health and yields. The aim of this study were to investigate the effects of volatile oils on some serum biochemical parameters, growth hormone activities and growth performance in broiler chickens. A total of 180 broilers were randomly divided into 6 groups and 3 replicates of 10 animals according to supplementation of thyme oil, rosemary oil, fennel oil, oil mixture 100 ppm dosage and oil mixture 200 ppm dosage. Serum growth hormone activity and glucose concentration were not affected by dietary addition of volatile oils according to groups. However, addition of rosemary oil, thyme oil and fennel oil showed an effect on decreased serum cholesterol and partially lipid concentrations in the present study. In conclusion, supplementation of volatile oils had impressive effects on serum total cholesterol and total lipids levels in broiler diets. Whereas, growth performance results were not affected by dietary addition of volatile oils in our study.Item Open Access Effect of sowing season and zinc sulfate application methods on quantity and quality characteristics of cicer arietinum(Uludağ Üniversitesi, 2015-04-28) Sharafi, SaeedGlobally, low zinc in soils are widespread. In order to investigation of sowing season and zinc sulfate application by different methods and times on quantity and quality characteristics of chickpea, an experiment was conducted with factorial arrangement based on randomized complete block design in 3 replications. Zinc sulphate (ZS) involved 8 levels (control; ZS0, soil application; ZSs , foliar spray in early flowering; ZSf, and foliar spray in early podding; ZSp and their components as ZSs ZSf , ZSs ZSp, ZSf ZSp, ZSs ZSf ZSp), with two planting seasons; F (fall), and S (spring). Soil application of Zinc sulfate was 25 kg ha-1 and foliar application of it was in concentration of 0.2: 1000. Results indicated that the effects of year, fertilizer treatments, planting dates and their interactions on yield were significant (a=1%). The maximum yield was obtained in treatment of F ZSS ZSf (soil application and one foliar spray of zinc sulfate in early flowering in fall sowing season) and treatment F ZSs (soil application of zinc sulfate in fall sowing season) with yield of 1524 and 1522 kg ha-1 , respectively. Results indicated that application of zinc sulfate increased Zn uptake, grain yield and protein content by soil application at fall planting time. It was also sowed that in fall sowing season, an application of zinc sulfate in preplanting (25 kg ha-1 ) and foliar spray in early flowering and in spring season, foliar spray in early flowering could be recommended.Item Open Access Ephemeral fever outbreak in a dairy herd in Adana region(Uludağ Üniversitesi, 2015-04-07) Çatık, Serkan; Cihan, Hüseyin; Özyiğit, M. Özgür; Şentürk, Sezgin; Uludağ Üniversitesi/Veteriner Fakültesi.The aim of the study was to report an unusual outbreak of bovine ephemeral fever with high mortality rate. The study was conducted in a dairy cattle herd in September 2012, around Çukurova region in southern Turkey. The herd was consisting of 550 dairy cattle (n=550). In herd, 95 cattle with different symptoms died within one week. Only 225 sick cows were examined by routine clinical procedure. Necropsy was performed on 3 dead animals with dyspnea score of 3. Following clinical examinations, blood samples were taken from the most severely affected 17 cows. A haemocell counter analyzed hematologic parameters. Clinically, fever, nasal discharge and dyspnea were the common symptoms. The clinical, hematological and pathological findings were comparatively evaluated. Unlike previous reported outbreaks, affected animals had extremely severe respiratory distress caused high mortality rate. At necropsy of 3 dead animals, emphysematous lung tissue, pneumonia and edema as well as petechial hemorrhages on serosal and subendocardial surfaces of the heart were noticed. Emphysema was also identified in the subcutaneous connective tissues. Taking the results of the study into consideration, it has been determined that ephemeral fever in dairy cattle has high mortality rate (over 20%) in Adana region.Item Open Access Asymptomatic prostatomegaly in a young anatolian shepherd dog(Uludağ Üniversitesi, 2015-04-07) Çeçen, Göksen; Goncagül, Gülşen; Kurt, Hasan; Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı.; Uludağ Üniversitesi/Mennan Pasinler Meslek Yüksekokulu.This case was aimed to report clinical, radiological, ultrasonographical and bacterial culture analysis results of a young dog with prostatomegaly. An 18-month-old age Anatolian shepherd dog undergoing treatment of chronic non-healing pressure wounds in hind leg was presented with a small amount of liquid stool and prolonged straining to defecate. Prostatomegaly was diagnosed radiographically and the other examinations confirmed the pathology. Chronic prostatitis caused by prostatomegaly had developed insidiously without prior bouts of acut prostatitis in this dog. The case was treated succesfully.Item Open Access Monitoring of sediment quality and nutrients dynamics of Lake Uluabat, Turkey(Uludağ Üniversitesi, 2015-04-07) Kurtoğlu, Sudan; Özengin, Nihan; Elmacı, Ayşe; Başkaya, Hüseyin Savaş; Uludağ Üniversitesi/Mühendislik Fakültesi/Çevre Mühendisliği Bölümü.Lake Uluabat, as being an important RAMSAR site, is located in Marmara Region, Turkey. Lake is one of Turkey's rich shallow lakes in terms of aquatic plants, fish and birds. The wetlands of Lake Uluabat and its catchments face a number of problems and threats which could make these valuable resources change or degrade. Human activities, both direct and indirect, are great contributors to degradation and loss of Lake Uluabat wetlands and its water quality. There is no law or regulations about sediment quality assessment and protection works in Lake Uluabat. The objective of this research is to evaluate the sediment quality and nutrients dynamics of Lake Uluabat. For this purpose pH, electrical conductivity, total nitrogen, hydrolysable total nitrogen, total phosphorus, available phosphorus, organic carbon parameters, anions (Cl- , CO3 = , CaCO3) and cations (Ca++, Mg++, Na+ , K+ ) of the sediment were analysed at five stations which are close to urban/industrial locations, inlet/outflow points of lake and drain point of Mustafakemalpasa Creek. The obtained results have shown that alkaline conditions are dominant in Lake Uluabat. The results of this study are to guide for sediment quality monitoring and assessment studies for Lake Uluabat and for further studies.Item Open Access In silico analysis of cis-regulatory elements on co-expressed genes(Uludağ Üniversitesi, 2015-04-07) Heidari, Parviz; Ahmadizadeh, Mostafa; Zarrini, Hamid NajafiCis-regulatory elements (CREs) are regions of non-coding DNA that regulate the transcription of nearby genes. CREs typically regulate gene transcription by functioning as binding sites for transcription factors. Publicly available database of co-expressed gene sets would be valuable tools for a wide variety of experimental designs, including targeting of genes for functional identification or for regulatory investigation. The study of CREs effect on expression can improve our understanding of co-expression genes and gene networks. In present study we compared the correlation between expression and CREs in co-expression genes with LTP5 by using the Genevestigator database that provides co-expressed genes deduced from microarray data, and SCOPE that uses to find CRDs in 800bp of upstream of the DNA sequences of co-expression genes. The result revealed that three motifs (TGSCAB, ATWTGYMG and CBTATC) of PRISM algorithm, GCCAC motif of BEAM and ATTGNVANNYGG motif of SPACER algorithm distribute in promoter of co-expressed genes and LUX transcription factor was identified by UniPROB database. We present here a new comparison method for detecting key cis-regulatory elements that effect on co-expressed genes to find a relation to clarify the function and regulation of particular genes and gene networks under stress conditions.