2013 Cilt 7 Sayı 21

Permanent URI for this collectionhttps://hdl.handle.net/11452/16997

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    MecA gene prevalence in staphylococcus aureus ısolates from dairy cows in Turkey
    (Uludağ Üniversitesi, 2013-01-09) Buyukcangaz, Esra; Kahya, Serpil; Şen, Aysin; Carli, K. Tayfun; Intas, K. Seyrek; Eyigor, Aysegul; Temelli, Seran; Uludağ Üniversitesi/Veteriner Fakültesi
    This cross-sectional study was performed between October 2010 and July 2012 from six different municipalities in Turkey to determine the prevelance of mecA gene in Staphylococcus aureus isolates from milk samples of mastitic cattle by PCR. Milk samples (n:1600) from 50 different Holstein Fresian herds were evaluated by California Mastitis Test and 480 clinical mastitis cases were diagnosed and taken for further investigation. Out of 480 samples, 151 have been identified to harbor Staphylococcus aureus by APIStaph® (Biomereux) identification panel and the results have been evaluated by API-web system. Kirby Bauer Disc Diffusion Test was used for determination of Cephoxitin® (Oxoid) susceptibility. By PCR, 24 isolates (15.89 %) were found to carry mecA gene and yielded an amplification product of 154 bp. This report represents that a significant number of MRSA was found among Staphylococcus aureus isolates in mastitis cases.