2010 Cilt 29 Sayı 1

Permanent URI for this collectionhttps://hdl.handle.net/11452/13938

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Biochemical factors ınfluencing autolysis of leuconostocs in buffer
(Uludağ Üniversitesi, 2010-07-12) Çıbık, Recep; Uludağ Üniversitesi/Veteriner Fakültesi.
Factors influencing autolysis of two dairy leuconostocs strains chosen for their moderate and relatively higher autolytic extend were investigated in buffer system. Higher level of autolysis was observed in bacteria that were harvested at earlier exponential phase. Autolysis was greatly influenced in acidic and basic pH values and optimal level was monitored between 6 and 7 values. Optimal incubation temperature to induce lysis was detected at 30°C, which is at the same time optimal growth temperature for leuconostocs strains. Whereas autolysis was activated by the presence of monovalent cations, an important level of inactivation was noted in the presence of divalent cations.
Evaluation of red blood cell and platelet ındices in cattle naturally ınfected with bovine viral diarrhea virus (BVDV)
(Uludağ Üniversitesi, 2010-07-08) Yeşilbağ, Kadir; Kocatürk, Meriç; Yılmaz, Zeki; Uludağ Üniversitesi/Veteriner Fakültesi.
The aim of this study was to evaluate red blood cell (RBC) and platelet indices in addition to white blood cell counts in cattle naturally infected with bovine viral diarrhea virus (BVDV). A total of 32 cattle, unvaccinated against BVDV, aged from 4 months to 9 months, were evaluated. Twenty two of them had clinical disorders regarding respiratory and gastrointestinal symptoms in which BVDV infection was virologically confirmed. Remaining 10 (BVDV free-cattle) were clinically healthy and used as controls. RBC indices (mean corpuscular volume [MCV], mean corpuscular hemoglobin [MCH], MCH concentration [MCHC], and RBC distribution width [RDW]) and platelet indices (plateletcrit [PCT], mean platelet volume [MPV], and platelet size distribution width [PDW]) were determined on a Cell-Dyn hematology analyzer. Hematological findings included neutrophilic leukocytosis or neutropenic leukopenia, lymphopenia, and monocytosis in BVDV-infected cattle. RBC, haemoglobin, and RDW were higher (P<.001) in infected cattle, but MCV was lower (P<.001), than those of controls. Platelet count, MPV, and PDW were higher in infected cattle, compared with controls (P<.05). In conclusion, changes in RBC and platelet indices may reflect changes in RBC and platelet production and reactivity. These indices should be used routinely during the diagnostic workup for BVDV infection in cattle practice.
Reducing the prevalence and severity of ınjurious pecking in laying hens without beak trimming
(Uludağ Üniversitesi, 2010-05-17) McKinstry, Justin Lee; Petek, Metin; Uludağ Üniversitesi/Veteriner Fakültesi.
Injurious pecking is a general term used to describe feather pecking, vent pecking, cannibalism and toe-pecking in laying hens. The severity of injurious pecking varies enormously, ranging from limited feather removal to cannibalism and death. Alternative housing systems for laying hens such as barn, free-range and aviary systems show much higher incidences of injurious pecking than with those birds housed in conventional caged system. From a welfare perspective injurious pecking can cause pain, stress, injuries, increased susceptibility to diseases, fear and death. Any major outbreak of injurious feather pecking can result in serious economic loss for the industry through decrease in egg production and feed efficiency. At present the egg industry uses both beak trimming of the birds and dim lighting methods to help reduce injurious pecking. However, both of these are being cricitised from a welfare point of view. In fact a general ban on beak trimming already exists in some European Union countries, with other countries considering a ban by 2011. Moreover, The EU ban on conventional laying cages (2012), when combined with a ban on beak-trimming, will increase the risk of injurious feather pecking and cannibalism. It is therefore important for future to consider other ways of controlling injurious pecking. Injurious pecking is a multi-factorial problem, which can be caused by environmental, genetic or nutritional factors and can be largely prevented by the use of a combination of environmental and husbandry management programs. This paper is intended to give a general overview of the potential risk factors and possible control measures associated with injurious pecking in laying hens, and in particular those flocks housed in non-cage systems.
Role of tissue lipoprotein lipase activity localization in the pelvic urethra in male cats
(Uludağ Üniversitesi, 2010-06-24) Dimitrov, Rosen Stefanov; Yonkova, Penka Yonkova; Stamatova, Kamelia Dimcheva
The pelvic urethra has been investigated in seven sexually mature, clinically healthy European shorthair male cats, aged 12-18 months, weighing 2.8-4 kg, obtained from a licensed animal breeder. All experiments were carried out under strict observance of the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes and law on Animal Protection in the Republic of Bulgaria. Cryostat cross sections of 5-7 µm were used for detection of lipoprotein lipase (LPL) by Tween method of Gomori. The reaction was positive when clusters of dark-brown lead sulfide precipitates were present. The localization of tissue LPL expression was determined by light microscopy. The intensity of the reaction was assessed by a semi-quantitative analysis using the score system. The enzyme histochemical investigation of feline pelvic urethra showed that the highest intenstiy of LPL expression, occurred in the muscular layer that was mainly of skeletal muscle. A medium intensity was observed in epithelial cells of disseminated part of the prostate and in the lumen of its glandular tubules. The urethral propria exhibited weak LPL expression, whereas no LPL activity was detected in the urethral lumen epithelium

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