2019 Cilt 38 Sayı 1

Permanent URI for this collectionhttps://hdl.handle.net/11452/14468

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The effect of saccharomyces cerevisiae and spirulina platensis on glutathione and leucocytes count in rabbits
(Bursa Uludağ Üniversitesi, 2018-02-22) Seyidoğlu, Nilay; Galip, Nurten; Serdar, Zehra; Savaş, Nilgün; Veteriner Fakültesi; Tıbbi Biyokimya Ana Bilim Dalı; 0000-0003-1714-410X; 0000-0002-0909-618X
Glutathione is the important antioxidant agent that is used for body detoxification system. Because of the fact that ıt is crucial for protecting health. A feeding trial was conducted to evaluate the effect of natural additives such as live yeast culture Saccharomyces cerevisiae (SC) and microalgae Spirulina platensis (SP) on the glutathione and leukocytes counts of rabbits. Forty male New Zealand white rabbits, aged 5-6 weeks, were studied in 4 groups. Treatments were control group, SC (added 3 g/kg diet), SP (added 5% of the diet) and, SC and SP (added 3 g/kg diet and added 5% of the diet) respectively. The experiment lasted for 90 days and the blood samples were obtained by ear venipuncture on the 90th day. In conclusion, according to the results of this study, although not statistically significant, supplementing rabbit with S. cerevisiae or S. platensis had increased on glutathione values. Glutathione tend to be positively correlated with the addition of SC or SP. No significant difference in white blood cell counts was evidenced, even if lymphocyte counts tended to increase and neutrophil counts to decrease in rabbits fed SC or SC+SP. The determination of biological consequences (antioxidant potential, resistance to diseases, and improvement of nutritional status) requires further investigations.
Comparison of serum IgG concentration, total protein, glutaraldehyde coagulation test and gamma glutamyl transferase in neonatal foals
(Bursa Uludağ Üniversitesi, 2018-04-15) Kasap, Sevim; Kennerman, Engin; Babaeski, Serdar; Dulger, Hüseyin; Veteriner Fakültesi; 0000-0001-9501-9983; 0000-0003-3877-2893
The purpose of the present study was to detect the passive transfer status in healthy neonatal foals by comparing serum immunoglobulin G (sIgG) concentration, serum total protein (STP), glutaraldehyde coagulation test (GCT) duration and gamma glutamyl transferase (GGT) activity . Fifteen neonatal foals (0-15 days old)’ blood samples were collected before suckling (day 0), 12th hour (hr), 24th hr (1st day), 7th and 15th days. Serum IgG and STP levels significantly increased after the 12th hr. Conversely, serum GCT duration significantly decreased (p< 0.05) in neonatal foals after the 12th hr. The result of the present study was shown that measurements of sIgG, STP concentration and GCT duration are useful parameters to detect Failure of Passive Transfer (FPT) in neonatal foals. While GCT and STP provide a simple and inexpensive field test, serum GGT measurement is not a beneficial test to determine colostrum intake in newborn foals.
Farklı dozlardaki gonadotropinlerin ovaryum follikül sayıları üzerine etkisi
(Bursa Uludağ Üniversitesi, 2018-07-26) Pancaroğlu, Güzin; İlhan, Tuncay; Erdost, Hatice; Veteriner Fakültesi; 0000-0003-3515-3552; 0000-0002-7327-9319; 0000-0003-1547-7293
Çalışmamızın amacı farelere, farklı dozlarda Pregnant Mare Serum Gonadotropin (PMSG) ve takiben Human Chorionic Gonadotropin (hCG) uygulamalarının, ovaryumda gelişmekte olan folikül ve korpus luteum sayılarına olan etkilerinin istatistiksel yöntemler ile belirlenmesidir. Çalışma materyali olarak 7 haftalık 40 adet BALC/c soyu dişi fare kullanıldı. Fareler, rastgele dört gruba ayrılarak, kontrol grubuna, 0,2 ml serum fizyolojik enjekte edildi. Diğer üç gruba subkutan yolla sırasıyla 2,5; 5 ve 7,5 I. U. PMSG hormonu verildi. PMSG enjeksiyonunun izleyen 48’inci saatte, deney gruplarına, PMSG’nin artan dozuna paralel olarak, gruplara sırasıyla 2,5; 5 ve 7,5 I. U. hCG, kontrol grubuna ise 0,2 ml serum fizyolojik subkutan yolla enjekte edildi. Anestezi altında alınan ve tespit edilen ovaryumlara rutin histolojik metodlar uygulanarak; ovaryum foliküllerinin ve korpus luteumların sayılması için Crossmon’ın üçlü boyama tekniği uygulandı. Çalışmamızda 3 farklı dozda yapmış olduğumuz uygulamalarda tüm deney gruplarında foliküler gelişimin kontrole oranla daha fazla olduğu görüldü. Gelişen folikül sayısının I. deney grubunda en çok olduğu sırasıyla III. ve II. deney grubu ile en az kontrol grubunda olduğu saptandı. Ovulasyon açısından korpus luteumlar değerlendirildiğinde en çok III. deney grubu sırasıyla II. ve I. deney grupları bulunurken en az kontrol grubunda olduğu görüldü. Uygulanan protokollerin hepsinde kullanılan gonadotropin dozuna bağlı olarak foliküler gelişimin ve ovulasyonun arttığı saptandı. Sonuç olarak; çalışmada en çok korpus luteum oluşumunun saptandığı III. deney grubunun (7,5 I.U. PMSG ve ardından 7,5 I.U. hCG’nin s.c.enjeksiyonunun) histolojik değerlendirmeler sonucunda, BALC/c soyu dişi fareler için en uygun süperovulasyon protokolü olduğuna karar verildi.
Genotip ve zemin tipinin etlik piliç büyüme performansı ve ekonomik verimlilik üzerine etkisi
(Bursa Uludağ Üniversitesi, 2018-09-10) Çavuşoğlu, Enver; Petek, Metin; Veteriner Fakültesi; 0000-0002-9018-3511; 0000-0003-4560-2438
The aim of this study was to evaluate the effects of genotype and floor type on bro iler growth performance and production eco nomics. In the study, slow-growing (Hubbard JA57) and fast-growing (Ross 308) broilers with slatted floor and deep litter were used, so there were four main groups (2x2), and each main group consisted of 5 replicates. Each replicate consisted of 10 male chicks, so 200 birds were used in total. The experiment lasted for 8 weeks. Live weight gain, feed consumption, and dead birds were recorded throughout the experiment. Variable costs of each group and its percentages within total costs were calculated, gross profit and profitability were calculated to analyze economic performance. As expected, the growth performance of fast-growing broiler was found to be better than slower-growings, and the effect of floor type on growth performance was found to be insignificant. In general, slow-growing genotypes were found to be better in terms of economic benefit. As a result of this study, even though fast-growing broilers had a better production performance, slowergrowing broilers had a better eco-nomic performance at the end of the experiment. However, considering that the slaughter ages of slowand fast-growing birds are different in commercial conditions, it would be more beneficial to make an economic comparison with the total production in the same unit in one year. Key words: Broiler, genotype, floor, growth performance
The influence of calving year on milk yield and milk components in dairy cattle
(Bursa Uludağ Üniversitesi, 2018-09-18) Kul, Ertuğrul; Çobanoğlu, Özden; Veteriner Fakültesi; 0000-0001-9633-634X
The aim of this study was to identify the effect of calving year on milk yield and milk composition traits in Holstein and Jersey cattle raised in the Marmara and Black Sea Regions of Turkey, respectively. The data set consisted of 582 dairy cows, which were 306 Holstein raised and 276 Jersey cows calving from 2011 to 2013. Except for fat yield, test day milk yield (TDMY), 305-day milk yield (305 DMY), fat percentage, protein percentage and protein yield of Jersey cows were affected by calving season. The effect of calving year on TDMY, 305 DMY, fat percentage, fat yield, protein percentage and protein yield of Holstein cows were found to be statically significant. In conclusion, in 2011 to 2013, milk yield and its composition in both Holstein and Jersey cows were significantly related to calving year.
Evaluation of some synthetic acaricides against varroa destructor (acari: varroidae) in Turkey: an ındication of resistance or misuse of fumigant amitraz?
(Bursa Uludağ Üniversitesi, 2018-10-15) Girişgin, Ahmet Onur; Özüiçli, Mehmet; Bah, Saidal Ali; Girişgin, Oya; Aydın, Levent; Karacabey Meslek Yüksekokulu; 0000-0002-0020-2708; 0000-0001-9896-1093
The parasitic mite, Varroa destructor is one of the most important agents for substantial losses in honeybee colonies throughout the world. Several acaricides consisting of synthetic and organic compounds are being used to combat mite. This study was conducted to determine the efficacies of three synthetic acaricides in naturally infested honeybee colonies at consecutive two autumn seasons. Acaricides were commercial preparations of coumaphos (liquid and plastic strips), amitraz (fumigation and plastic strip) and flumethrin (plastic and wooden strips) as a treatment group consisting of eight hives per drug. A control group was kept in both seasons. All drugs were applied as prescribed to the homogenised Varroa-infested honeybee colonies at consecutive two years. The evaluation of efficacies was based on the collected mite percentage obtained with powdered sugar method and it was calculated through Henderson-Tilton’s formula. Dropped mites onto the pollen drawers were also evaluated statistically and drugs were compared to each other. Results showed us the effective drugs (up to 90%) were amitraz plastic strip, flumethrin wooden strip and coumaphos plastic strip with 98.5%, 96.5%, and 93.2% averages, respectively according to formula if evaluated within two autumn seasons. Fumigation of amitraz is not sufficient if compared to others and the control group. This data is discussed for suspicion of the possible resistance of mites or misuse of the product with this kind of application.
Screening of veterinary growth-promoting agent and antibacterial residues in beef cattle and broiler meats
(Bursa Uludağ Üniversitesi, 2019-02-18) Çaycı, Meltem; Kılıç, Ayşe Sena; Oruç, Hasan Hüseyin; Sarıyev, Rashad; Sağlık Bilimleri Enstitüsü; 0000-0002-3401-9186; 0000-0001-6430-5629; 0000-0002-5399-2395; 0000-0002-1129-4118
This study was aimed to determine residues of growth-promoting agents and some antibacterials in beef cattle and broiler meats consumed in Bursa, as well as to evaluate their hazards on public health. A total of 45 meat samples consisting of 36 beef cattle meat and 9 broiler meat samples were collected from supermarkets and butchers between November and December in 2016. The analysis was carried out by biochip array-based immunoassay technique. This system is also currently used for simultaneous detection and quantitation of different anabolics consisting of β-agonists, boldenone, corticosteroids, nandrolone, ractopamine, stanozolol, stilbenes, trenbolone and zeranol, and six group of antimicrobials consisting of quinolones, cephalosporins, amphenicols, aminoglycosides, macrolides and tetracyclines. Although residues of growth-promoting agents could not be detected in any of the samples, antimicrobial residues from all groups were detected in 10 beef cattle meat samples and tetracycline residues were detected in two broiler meat samples at various levels. In conclusion, there is no risk to consumers for growth-promoter residues according to the results. The detected antibacterial levels were generally lower than hazardous concentrations of residue. However, some detected levels for quinolone, amphenicol, macrolide and tetracycline groups in beef meat samples, and detected concentrations for tetracycline group in two broiler meat samples exceeded the maximum residue limits, and could pose a risk for public health.
Survey of ochratoxin a in coffee, dried grapes and grape pekmez samples in Burdur, Turkey
(Bursa Uludağ Üniversitesi, 2019-03-10) Yurdakul, Özen Kursun; Kocasarı, Fatma Şahindokuyucu; Yalçın, Halil; Keyvan, Erhan
The aim of this study was to investigate the occurrence and levels of ochratoxin A (OTA) in coffee (roasted and instant), dried grapes and grape pekmez samples consumed in Burdur city markets. During 2015, a total of 86 samples including 43 coffee (30 instant coffee and 13 roasted coffee), 17 dried grapes and 26 grape pekmez were randomly collected from different markets of Burdur. The occurrence and contamination levels of OTA in the samples were investigated by the competitive enzyme-linked immunoabsorbent assay (ELISA) method. OTA was detected in 24 (55 %) coffee samples (13 roasted coffee and 11 instant coffee samples) and in 1 (3 %) grape pekmez samples. The range OTA levels were 8.34 and 22.54 µg/kg in coffee samples and 20.48 µg/kg in one grape pekmez sample, respectively. The highest recorded OTA concentration was 22.54 µg/kg in instant coffee. Furthermore, 13 roasted coffee, 11 instant coffee and 1 grape pekmez samples were contaminated at levels above the Turkish legal limits of 5 µg/kg,10 µg/kg and 2 µg/kg, respectively. In contrast, OTA was not detected in all dried grape samples. It is concluded that the occurrence of OTA, coffee samples, in particular may be considered as a possible hazard for public health.
The utilization areas of cabergoline in veterinary gynecology
(Bursa Uludağ Üniversitesi, 2019-04-14) Yenilmez, Kudret; Eren, Nihal
Cabergoline is a potent dopamine agonist and a synthetic ergot derivative, and it acts by inhibiting prolactin secretion from the pituitary gland. It reduces the amount of plasma prolactin and inhibits progesterone secretion at the same time. Because of these effects, it has found a widespread area of use in the treatment of hyperprolactinemic disorders both in the human medicine and veterinary medicine.
Effect of central and peripheral ınjected nesfatin-1 on electrocardiography in rats
(Bursa Uludağ Üniversitesi, 2019-04-14) Çiftçe, Kübra; Güvenç, Gökçen; Bas, Ayşenur; Yalçın, Murat; Veteriner Fakültesi; 0000-0002-2647-0946; 0000-0002-1413-3651; 0000-0002-7825-4823; 0000-0002-6605-3514
Nesfatin-1 is an anorexic nucleobindin-2 -derived peptide and it has directly and centrally effect on the heart. The current study was designed to determine the effect of centrally and peripherally administered nesfatin-1 on electrocardiography (ECG) of healthy both fasted rats for 12 h and satiated rats fed ad libitum. In order to record ECG, the electrodes were placed limbs of at lead II under ketamine (50 mg/kg; im) and xylazine (20 mg/kg; im) anesthesia mix. Centrally administered different doses of nesfatin-1 (100 and 200 pmol; icv) resulted in dose- and time-dependently a statistically significant increase (p <0.05) in T wave, Q-T interval, and R-R interval duration without changing in ECG waves’ amplitude in both satiated and fasted rats. In a similar way, peripheral administration of nesfatin-1 (80 μg/kg; iv) in satiated rats prolonged statistically significant (p <0.05) T wave, Q-T interval, and R-R interval without producing a change in ECG waves’ amplitude. Moreover, icv administered nesfatin-1 in fasted and satiated rats, and iv injected nesfatin-1 in satiated rats induced a statistically significant decrease in heart rate (p <0.05). In conclusion, our findings suggest that centrally and peripherally administrated nesfatin-1 caused a delay in T wave, Q-T interval and two R-waves interval duration in ECG so that leading to a bradycardic effect in heart rate.
The study of histopathologic changes of experimental ınfection with listonella (vibrio) anguillarum in rainbow trout
(Bursa Uludağ Üniversitesi, 2019-04-14) Ceylan, Mustafa; İnan, Sevda; Satıcıoğlu, İzzet B.; Duman, Muhammed; Özyiğit, Özgür; Altun, Soner; Veteriner Fakültesi; 0000-0003-0682-8127
The rainbow trout production increased more than 100% in the last decade and total rainbow trout production was shown as 107.013 tons according to 2016 statics. According to Federation of European Aquaculture Producer, Turkey was determined as the biggest rainbow trout producer into the European Countries in 2016. Together with high production capacity, a number of outbreaks were reported causing L. anguillarum (Vibriosis). The pathogenesis of L. anguillarum which were experimentally infected in rainbow trout were examined during 15 days in our study. Spreading of agent that was injected with intraperitoneally into tissue and organs were studied by using histopathological methods. The mortality rate of agent was determined above 70% and deaths were seen in 2-3th days of experiment. In addition of these, liver, spleen, kidney and gills were determined as the most affected organs and tissues. In the present study was obtain for original pathological findings of L. anguillarum.

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